Amelogenin as a regenerative endodontic molecule for immature teeth with apical periodontitis. An experimental study

Mounir, Maha M. F.; Rashed, Fatma; Bukhary, Sahar

doi:10.1016/j.jobcr.2022.08.008

Cited by 2 publications

(3 citation statements)

References 23 publications

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“…According to previous work, amelogenin, the main component of Emdogain, regulates cell signaling pathways by activating Wnt/β-catenin signaling [40]. Pluripotent Oct4 and Sox2 factors show higher expression levels in the apical papilla and can enhance pulp regeneration in the presence of this protein [40]. Other findings indicate that amelogenin exon 5 peptide promotes the expression of MAPK signaling pathway proteins, with enhanced cell proliferation and osteogenic differentiation [41].…”

Section: Discussionmentioning

confidence: 96%

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The Combined Effects of Hydraulic Calcium Silicate Cement and Enamel Matrix Derivative Regarding Osteogenic and Dentinogenic Differentiation on Human Dental Pulp Stem Cells

2023

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The ideal treatment option for immature necrotic permanent teeth is regeneration of the pulp–dentin complex. Mineral trioxide aggregate (MTA), the conventional cement used for regenerative endodontic procedures, induces hard tissue repair. Various hydraulic calcium silicate cements (HCSCs) and enamel matrix derivative (EMD) also promote osteoblast proliferation. The purpose of the present study was to determine the osteogenic and dentinogenic potential of commercially distributed MTA and HCSCs when applied in combination with Emdogain gel on human dental pulp stem cells (hDPSCs). The presence of Emdogain resulted in greater cell viability, and higher alkaline phosphatase activity was detected in the Emdogain-supplemented groups in the early days of cell culture. On qRT–PCR, the groups treated, respectively, with Biodentine and Endocem MTA Premixed in the presence of Emdogain showed an increased expression of the dentin formation marker DSPP, and the group treated with Endocem MTA Premixed in the presence of Emdogain showed an upregulated expression of the bone formation markers OSX and RUNX2. In an Alizarin Red-S staining assay, all of the experimental groups exhibited a greater formation of calcium nodules when treated in combination with Emdogain. Overall, the cytotoxicity and osteogenic/odontogenic potential of HCSCs were similar to that of ProRoot MTA. The addition of the EMD increased the osteogenic and dentinogenic differentiation markers.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

The Combined Effects of Hydraulic Calcium Silicate Cement and Enamel Matrix Derivative Regarding Osteogenic and Dentinogenic Differentiation on Human Dental Pulp Stem Cells

2023

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“…Indeed, the complex pathophysiology of endodontic and periodontal conditions provides several potential molecular targets to modulate inflammation and tissue destruction. For example, a recent study highlights the positive effects of amelogenins on apical healing and pulpal regeneration when used as intracanal medication [ 73 ]. Further studies should be carried out using a more elaborate combined ex vivo/in vitro model to gain a better understanding of the pathophysiology of EPLs and the response of periodontal tissues in the presence of ICMs under conditions closer to reality.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study

Sy,

Chevalier,

Maton

et al. 2023

Antibiotics

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Endo-periodontal lesions are challenging clinical situations where both the supporting tissues and the root canal of the same tooth are infected. In the present study, chlorhexidine (CHX)-loaded calcium hydroxide (CH) pastes were used as intracanal medications (ICMs). They were prepared and tested on pathogens found in both the root canal and the periodontal pocket. Exposure to 0.5% and 1% CHX-loaded ICMs decreased the growth of Porphyromonas gingivalis and was effective in eradicating or inhibiting an Enterococcus faecalis biofilm. CH was injected into the root canal of extracted human teeth immersed in deionized water. CHX-loaded ICMs resulted in the transradicular diffusion of active components outside the tooth through the apex and the lateral dentinal tubules, as shown by the release of CHX (from 3.99 µg/mL to 51.28 µg/mL) and changes in pH (from 6.63 to 8.18) and calcium concentrations (from 2.42 ppm to 14.67 ppm) after 7 days. The 0.5% CHX-loaded ICM was non-toxic and reduced the release of IL-6 by periodontal cells stimulated by P. gingivalis lipopolysaccharides. Results indicate that the root canal may serve as a reservoir for periodontal drug delivery and that CHX-based ICMs can be an adjuvant for the control of infections and inflammation in endo-periodontal lesions.

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Amelogenin as a regenerative endodontic molecule for immature teeth with apical periodontitis. An experimental study

Cited by 2 publications

References 23 publications

The Combined Effects of Hydraulic Calcium Silicate Cement and Enamel Matrix Derivative Regarding Osteogenic and Dentinogenic Differentiation on Human Dental Pulp Stem Cells

The Combined Effects of Hydraulic Calcium Silicate Cement and Enamel Matrix Derivative Regarding Osteogenic and Dentinogenic Differentiation on Human Dental Pulp Stem Cells

Therapeutic Potential of Chlorhexidine-Loaded Calcium Hydroxide-Based Intracanal Medications in Endo-Periodontal Lesions: An Ex Vivo and In Vitro Study

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