Amenability of castor to an Agrobacterium-mediated in planta transformation strategy using a cry1AcF gene for insect tolerance

Arthikala, Manoj‐Kumar; Sreevathsa, Rohini; Reddy, Kalpana N.; Ganesh, Prasa Trichy; Udayakumar, M.

doi:10.1007/s12892-010-0013-z

Cited by 24 publications

(5 citation statements)

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“…Non-tissue culture-based transformation strategies using apical meristem facilitated in the development of stable transgenics in several recalcitrant crop species ( Kumar et al, 2011 ; Keshavareddy et al, 2013 ; Singh et al, 2018 ). One such apical meristem-targeted in planta transformation approach was exploited in the present study for the development of transformants in cotton ( Rohini and Rao, 2002 ; Keshamma et al, 2008 ; Karthik and Rohini, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

et al. 2020

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Weeds burden plant growth as they compete for space, sunlight, and soil nutrients leading to 25-80% yield losses. Glyphosate [N-(phosphonomethyl)glycine] is a widely used broad spectrum non-selective herbicide that controls weeds by inhibiting 5enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme and interfering with the shikimate biosynthesis pathway. Cotton (Gossypium hirsutum L.) is one of the most important commercial crops grown worldwide for its fiber. We have developed herbicide tolerant transgenic cotton (cv. P8-6) by introgression of a codon-optimized and modified EPSPS gene (CP4-EPSPS) possessing an N-terminal chloroplast targeting peptide from Petunia hybrida. Because of the recalcitrant nature of cotton, a genotype-independent non-tissue culture-based apical meristem-targeted in planta transformation approach was used to develop transformants. Although in planta transformation methodologies are advantageous in developing a large number of transgenic plants, effective screening strategies are essential for initial identification of transformants. In the present study, the use of a two-level rigorous screening strategy identified 2.27% of T1 generation plants as tolerant to 800 and 1,500 mg/L of commercially available glyphosate (Roundup). Precise molecular characterization revealed stable integration, expression, and inheritance of CP4-EPSPS in advanced generations of the promising transgenic events. Further, superiority of selected transgenic plants in tolerating increasing levels of glyphosate (500-4,000 mg/L) was ascertained through reduced accumulation of shikimate. This report is the first of its kind where cotton transformants tolerating high levels of glyphosate (up to 4,000 mg/L) and accumulating low levels of shikimate have been identified. This study not only reiterated the genotype-independent nature of the transformation strategy but also reiterated the translational utility of the CP4-EPSPS gene in management of weeds.

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Section: Introductionmentioning

confidence: 99%

Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

et al. 2020

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“…Earlier, transgenic castors depicted higher resistance to neonate larvae castor semilooper on transformed plant with integration of the cry1Ab and bar genes through Agrobacterium genetic transformation (B. MALATHI & al [13]). Similarly, bioassay of castor semilooper and S. litura larvae showed varying levels of larval mortality and slow growth in larvae feed on transgenic plants leaf tissues then control plants which were transformed with Bt chimeric gene cry1EC, cry1AcF, and cry1Aa by Agrobacterium-mediated genetic transformation methods in the laboratory (M. SUJATHA & al [16]; A.M. KUMAR & al [14]; T. MUDDANURU & al [39]).…”

Section: Insect Bioassay Of Transformed Castor Plants With S Litura U...mentioning

confidence: 99%

“…Different genetic transformation methods were used to confer insect pest resistance to transgenic castors, including Agrobacterium-mediated (M. SUJATHA, M. SAILAJA [7]; B. MALATHI & al [13]; A.M. KUMAR & al [14]); Direct gene (M. SAILAJA al [15]); containing the Bt genes cry1Ab (B. MALATHI & al [13]), cry1EC (M. SUJATHA & al [16]), chimeric cry1AcF Agrobacterium-mediated genetic manipulation protocols have also been successfully optimised and applied to industrially relevant crops such as Cajanus cajan (M.J. PAREKH & al [17]), Ocimum gratissimumi (S. KHAN & al [18]), Gossypium hirsutum (V.H. SOLANKI & al [19]; V. KHANDELWAL & al [20]), Trachyspermum ammi (M. NIAZIAN & al [21]), Oryza sativa (V. SRIVASHTAV & al [22]) and Camellia sinensis (H.R.…”

Section: Introductionmentioning

confidence: 99%

An Efficient Agrobacterium-mediated Genetic Transformation Using cry1F gene in Castor (Ricinus communis L.) for protection Against lepidopteran Insects

KANSARA,

JHA,

SOLANKI

et al. 2022

RBL

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to foliage feeders like Spodoptera litura which resulted in a loss of production. This report focuses on the development of an optimized protocol for the transformation of castor shoot apices by Agrobacterium tumefaciens strain LBA4404 containing plasmid construct pBIN1F harboring neomycin phosphotransferase (nptII), as selectable marker gene and Bacillus thuringiensis var. aizawai (Bt) cry1F gene controlled by cauliflower mosaic virus 35S promoter. Several parameters like O.D., concentration of kanamycin and acetosyringone were optimized and produced a significant difference in the transformation efficiency. Co-cultivation time and seedling age were factors, with overall transformation efficiency of 2.0% in 15 days-old seedlings and co-cultivated for 3 days. The surviving and actively developing shoot apices were validated for gene integration by molecular analysis after being preliminarily screened on kanamycin. Furthermore, PCR, qRT-PCR and insect bioassay were used to confirm the putative primary transformants. When bioassayed against newly hatched Spodoptera litura hatchlings, these putative transgenics with cry1F gene caused significant (≤93%) insect mortality. Cry1F gene expressing transgenic plants had adequate defence against Spodoptera litura when exposed to castor.

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“…Trying to face Spodoptera litura, [77] recently reported on the development of an A. tumefaciens mediated in planta transformation protocol for castor bean. These authors developed transgenic lines from 2-day-old seedlings infected with Agrobacterium, EHA105/pBinBt8 harboring cry1AcF gene and then, molecular and expression analysis confirmed the transgenic nature and identified high-expressing plants.…”

Section: Castor Bean (Ricinus Communis L)mentioning

confidence: 99%

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

Villanueva-Mejía¹,

Álvarez²

2017

Advances in Seed Biology

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In 2009, big challenges facing the agricultural sector in the twenty-first century were presented to the world. Human population growth, increased life expectancy, loss of biodiversity, climate change and accelerated land degradation are the main factors contributing to rethink agriculture system production. In that scenery, modern biotechnology has set a stage for the advancement of agricultural practices and it is clearly an important ally to apply a broad array of technologies and innovative systems where they are most needed, such as enhancing crop productivity, increasing yields, and ultimately ensuring food security. One of the biggest challenges is related to technify production systems, but with no doubt, developing genetic improvement toward getting an efficient and sustainable agriculture, generating new seed qualities (new traits), such as, among others, to upset fatty acids content in oilseed crops have been growing up significantly due to industry interest. In this study, a review about the main advances in genetic improvement of some oilseed crops, starting with omics to understand metabolic routes and to find out key genes in seed oil production, and also, getting in use of modern biotechnology to alter the production of fatty acids, and to face biotic challenges in oilseed crops is presented.

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Amenability of castor to an Agrobacterium-mediated in planta transformation strategy using a cry1AcF gene for insect tolerance

Cited by 24 publications

References 24 publications

Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

An Efficient Agrobacterium-mediated Genetic Transformation Using cry1F gene in Castor (Ricinus communis L.) for protection Against lepidopteran Insects

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

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