Amikacin, Ceftazidime, and Flucloxacillin against Suspended and Adherent Pseudomonas aeruginosa and Staphylococcus epidermidis in an In Vitro Model of Infection

Vergères, Patrice; Blaser, Jürg

doi:10.1093/infdis/165.2.281

Cited by 37 publications

(31 citation statements)

References 25 publications

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“…(i) System. A previously described pharmacokinetic in vitro model was used to expose bacterial cultures to oscillating concentrations of antimicrobial agents (22). In brief, 17-ml culture compartments were continuously perfused with tryptic soy broth (TSB) supplemented with 50 g of Ca 2ϩ per ml and 25 g of Mg 2ϩ per ml (TSB-S).…”

Section: Methodsmentioning

confidence: 99%

“…Inocula of adherent bacteria inocula were set as biofilm on 12 sinter glass beads (22). These beads consist of pieces of glass which have been pressed together to form a porous ball.…”

Section: Methodsmentioning

confidence: 99%

“…One bead was removed from the culture compartment with a sterile surgical forceps at time zero and at 2, 6, 24, 26, 30, and 48 h and placed in tubes containing 2 ml of sterile broth. At least 90% of the adherent bacteria were detached from the glass surface by vigorously vortexing the tubes (22). The number of CFU of these suspended bacteria was determined.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a pharmacodynamic model that quantitatively considers the activity of antimicrobial agents against both adherent and suspended bacteria, mimicking prolonged bacterial exposure to oscillating drug levels according to human serum kinetics, has been presented. Differences in response between suspended and adherent inocula have been found for some regimens and strains (22).…”

mentioning

confidence: 99%

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In vivo verification of in vitro model of antibiotic treatment of device-related infection

Blaser

Vergères

Widmer

et al. 1995

Antimicrob Agents Chemother

112

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Device-related infections are difficult to treat with antibiotics alone. Standard susceptibility tests do not correlate with treatment success. Therefore, the utility of a pharmacokinetic in vitro model has been evaluated in comparison with the tissue-cage infection model in guinea pigs. The bactericidal activity of 28 treatment regimens has been studied by using three different test strains. In vitro efficacy was defined as reduction in the number of suspended or adherent bacteria, and in vivo efficacy was defined as reduction in the number of bacteria in tissue-cage fluid. Test results between the two models (in vivo and in vitro) correlated well, with correlation coefficients of 0.85 for in vivo efficacy versus in vitro efficacy against suspended bacteria and 0.72 for in vivo efficacy versus in vitro efficacy against adherent bacteria (P < 0.05) for Staphylococcus aureus, 0.96 and 0.82 (P < 0.05) for Staphylococcus epidermidis, and 0.89 and 0.97 for Escherichia coli, respectively. In contrast, standard susceptibility tests, ratios of MICs to trough or peak levels, ratios of the area under the curve to the MIC, or time above the MIC were not predictive for therapeutic outcome in either the in vitro or in vivo model. In both models, the bactericidal activity levels with combination regimens were significantly higher than those with single-drug regimens (P < 0.001). Furthermore, rifampin combinations with either vancomycin, teicoplanin, fleroxacin, or ciprofloxacin were significantly more bactericidal against adherent bacteria than netilmicin combinations with vancomycin or daptomycin (P < 0.01). Thus, in vivo verification of the pharmacokinetic in vitro model correlated well with the animal model. The in vitro model offers an alternative to the animal model in experiments that screen and assess antibiotic regimens against devicerelated infections.

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Section: Methodsmentioning

confidence: 99%

“…Inocula of adherent bacteria inocula were set as biofilm on 12 sinter glass beads (22). These beads consist of pieces of glass which have been pressed together to form a porous ball.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

In vivo verification of in vitro model of antibiotic treatment of device-related infection

Blaser

Vergères

Widmer

et al. 1995

Antimicrob Agents Chemother

112

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“…In this case it has been reported that S. aureus and relatives (e.g. Staphylococcus epidermidis) cause 60% of the nosocomial infections and show the highest resistance to currently used antibiotics when growing as biofilms [50,59]. Therefore, it is of the utmost importance to find new potent antibiotics to challenge these "super bugs".…”

Section: Antimicrobial Activity Of H Illucens Larval Extracts On Bacmentioning

confidence: 99%

The black soldier fly, Hermetia illucens – a promising source for sustainable production of proteins, lipids and bioactive substances

Müller

Wolf

Gutzeit

2017

Zeitschrift Für Naturforschung C

183

134

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Abstract:The growing demand worldwide for proteins and lipids cannot be met by the intensive use of agricultural land currently available. Insect mass cultures as a source for proteins and lipids have been in focus for various reasons. An insect with many positive properties is the black soldier fly, Hermetia illucens, whose larvae could be used for the sustainable production of proteins and lipids. Furthermore, the larvae produce bioactive substances which could potentially be used for human and animal welfare.

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Pharmacodynamic In Vitro Models to Determine the Effect of Antibiotics

Michael

Barth

Kloft

et al. 2013

Fundamentals of Antimicrobial Pharmacokinetics and Pharmacodynamics

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Pharmacodynamic in vitro models are widely used to evaluate the antibiotic effect against microorganisms. With them it is possible to have full control of the drug profi le (static or dynamic) within the system and to obtain the correspondent effect at a certain time period. Based on the experimental conditions and results a mathematical model describing the relationships between the effective concentration of the drug, the pharmacological effect, and time can be obtained. By means of simulations from the model it is possible to simulate and predict effects that optimize the treatment for defi ning the optimal dosage regimens. Once this information is available it can be used to support dose selection in a more rational manner.As a prerequisite, it is important to evaluate the different pharmacodynamic models, considering the advantages and disadvantages, as well as the objective of the proposed study. Therefore this review focuses on the description of the general requirements for pharmacodynamic models providing an overview on up to now developed in vitro models.

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Amikacin, Ceftazidime, and Flucloxacillin against Suspended and Adherent Pseudomonas aeruginosa and Staphylococcus epidermidis in an In Vitro Model of Infection

Cited by 37 publications

References 25 publications

In vivo verification of in vitro model of antibiotic treatment of device-related infection

In vivo verification of in vitro model of antibiotic treatment of device-related infection

The black soldier fly, Hermetia illucens – a promising source for sustainable production of proteins, lipids and bioactive substances

Pharmacodynamic In Vitro Models to Determine the Effect of Antibiotics

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