Amine oxidases as important agents of pathological processes of rhabdomyolysis in rats

Gudkova, O A; Latyshko, N. V.; Shandrenko, S. G.

doi:10.15407/ubj88.01.079

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…AO is regarded as a biological regulator, especially for cell growth and differentiation ( 44 ). AO is also an important agent of pathological processes of rhabdomyolysis in rats ( 45 ). In mammals, the SPLA2 families are involved in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome responses of RNAi-mediated ETH knockdown in Scylla paramamosain at different premolt substages

Chan

Wen

et al. 2022

Front. Endocrinol.

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Ecdysis triggering hormone (ETH) plays an important role in molting, reproduction, and courtship behavior in insects. To investigate the potential downstream pathways and genes of ETH in Scylla paramamosain, RNA interference (RNAi) was conducted on crabs at early (D0) and late (D2) premolt substages, and the transcriptome profiles of each group were compared by RNA sequencing. Real-time quantitative polymerase chain reaction (RT-qPCR) and semiquantitative polymerase chain reaction (RT-PCR) results showed a significant knockdown of ETH at D0 stage, whereas a significant increase was shown conversely in crabs at D2 substage after the injection of dsETH. A total of 242,979 transcripts were assembled, and 44,012 unigenes were identified. Transcriptomic comparison between crabs at D2 and D0 substages showed 2,683 differentially expressed genes (DEGs); these genes were enriched in ribosome and pathways related to transcription factor complex and cell part. Twenty DEGs were identified between dsETH-injected and dsGFP-injected crabs at D0 substage; these DEGs were involved in carbohydrate metabolism, one carbon pool by folate, and chitin binding. Twenty-six DEGs were identified between dsETH-injected and dsGFP-injected crabs at D2 substage; these DEGs were involved in calcium channel inhibitor activity, fat digestion and absorption, and cardiac muscle contraction. RT-qPCR verified the differential expression of the selected genes. In conclusion, crabs at D0 substage are more active in preparing the macromolecular complex that is needed for molting. Moreover, ETH has potential roles in carbohydrate metabolism, one carbon pool by folate, and chitin binding for crabs at D0 substage, while the role of ETH turns to be involved in calcium channel inhibitor activity, fat digestion and absorption, and cardiac muscle contraction at D2 substage to facilitate the occurrence of molting. The selected DEGs provide valuable insight into the role of ETH in the regulation of crustacean molting.

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Section: Discussionmentioning

confidence: 99%

Transcriptome responses of RNAi-mediated ETH knockdown in Scylla paramamosain at different premolt substages

Chan

Wen

et al. 2022

Front. Endocrinol.

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“…Earlier, we used animal model of rhabdomyolysis, which is accompanied by powerful oxidative stress due to the release of labile ferric iron (Fe 3+ ) from myoglobin/hemoglobin of ruptured myocytes that led to acute kidney failure. It was demonstrated that all tested amine oxidases, and SSAO to the great extent, play an essential role in the kidney destruction under these conditions [15]. The data formed the basis for the hypothesis on the involvement of the enzyme in the development of other kidney pathologies.…”

mentioning

confidence: 83%

“…Assay for SSAO activity. Fluorometric assay designed earlier [15] was used to estimate activity of the amine oxidase in tissue microsomes and blood. In brief, after preincubation of the enzyme preparations with monoamine oxidase inhibitor pargiline (1×10 -4 M) at room temperature for 20 min, followed by incubation for 30 min at 25°C with substrate (0.1 mM methylamine), the reaction was terminated by addition of 30% ZnSO 4 .…”

Section: Methodsmentioning

confidence: 99%

Rhabdomyolysis attenuates activity of semicarbazide sensitive amine oxidase as the marker of nephropathy in diabetic rats

Hudkova¹,

Krysiuk²,

Drobot³

et al. 2022

Ukr. Biochem. J.

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amine oxidases are involved in the progression of many diseases and their complications, including renal failure, due to the generation of the three toxic metabolites (H 2 O 2 , aldehydes, and ammonia) in the course of biogenic amines oxidative deamination. The participation of the first two products in kidney pathogenesis was confirmed, whereas the role of ammonia as a potential inducer of the nitrozative stress is not yet understood. The aim of the present study was to test how further intensification of oxidative stress would affect diabetes-mediated metabolic changes. For this purpose, a rat model of glycerol-induced rhabdomyolysis, as a source of powerful oxidative stress due to the release of labile Fe 3+ from ruptured myocytes, on the background of streptozotocin-induced diabetes was used. The experimental animal groups were as follows: group 1 -'Control', group 2 -'diabetes', group 3 -'diabetes + rhabdomyolysis'. a multifold increase in semicarbazide sensitive amine oxidase (SSAO) activity in the kidney and blood, free radicals (FR), MetHb and 3-nitrotyrosine (3-NT) levels in the blood, as well as the emergence of HbNO in plasma and dinitrosyl iron complexes (DNICs) in the liver of animals in group 2 as compared to control were revealed. An additional increase in FR, HbNO levels in the blood, and DNICs in the liver of animals in the diabetes + rhabdomyolysis group as compared to the diabetes group, which correlated with the appearance of a large amount of Fe 3+ in the blood of group 3 animals, was detected. Unexpectedly, we observed the positive regulatory effects in animals of the diabetes + rhabdomyolysis group, in particular, a decreased SSaO activity in the kidney and 3-NT level in plasma, as well as the normalization of activity of pro-and antioxidant enzymes in the blood and liver compared to animals of diabetes group. these consequences mediated by rhabdomyolysis may be the result of NO exclusion from the circulation due to the excessive formation of NO stable complexes in the blood and liver. The data obtained allow us to consider SSAO activity as a marker of renal failure in diabetes mellitus. In addition, we suggest a significant role of nitrosative stress in the development of pathology, and, therefore, recommend NO-traps in the complex treatment of diabetic complications.

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“…Fluorometric assay was used to estimate activity of all AOs under the investigation (SSAO, DAO, PAO and LOX). The method is based on the formation of an intense fluorophore between folic acid and hydrogen peroxide released during the oxidation of the corresponding amine as a substrate [18,19].…”

Section: Mg/kg)mentioning

confidence: 99%

Semicarbazide diminishes the signs of bleomycin-induced pulmonary fibrosis in rats

Hudkova¹,

Krysiuk²,

Kishko³

et al. 2021

Ukr.Biochem.J.

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The pathogenesis of pulmonary fibrosis (PF) is accompanied by extracellular matrix (ECM) deposition, oxidative stress, and inflammation progression, as well as hyperactivation of amine oxidases (AOs), which contribute to disease manifestation. The present study aims to elucidate the effect of semicarbazide (SC), an inhibitor of Cu-containing AOs: lysyl oxidase (LOX), semicarbazide sensitive amine oxidase (SSAO), diamine oxidase (DAO), on PF induced in rats by bleomycin (BLM). Eighteen male Wistar rats were randomly divided into four groups: Control, rats of BLM group received BLM (5 mg/kg, intratracheally once), BLM+SC group obtained 0.005% solution of SC (about 50 µg per capita per day) for three weeks starting immediately after BLM injection, and the Control+SC group drank the same solution as BLM+SC group. The content of cross-linked collagen in total bronchi and free radicals in lung, activities of LOX, SSAO, DAO, polyamine oxidase (PAO), Cu, Zn-superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPx) in lung and blood were measured. BLM injection induced PF that was confirmed histologically and morphometrically as well as by the elevation of the content of cross-linked collagen and free radicals. The activities of LOX and SSAO involved in post-translational modification of ECM and inflammation were significantly increased (P < 0.05). The activities of DAO, and PAO that control polyamine metabolism were also essentially raised. Among antioxidant enzymes, only GPx was activated in the BLM group as compared to control. These changes were absent in the BLM+SC group. SC intake promoted the fact that the histology and morphometric parameters of lung tissue, the content of cross-linked collagen in the bronchi and free radicals in the lung, as well as the activity of the studied enzymes remained at the control level. Our data suggest that SC suppresses the development of BLM-induced PF by inhibiting AOs activities. K e y w o r d s: pulmonary fibrosis, bleomycin, amine oxidases, redox state, antioxidant enzymes, semicarbazide.

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Amine oxidases as important agents of pathological processes of rhabdomyolysis in rats

Abstract: In this study we

Cited by 3 publications

References 24 publications

Transcriptome responses of RNAi-mediated ETH knockdown in Scylla paramamosain at different premolt substages

Transcriptome responses of RNAi-mediated ETH knockdown in Scylla paramamosain at different premolt substages

Rhabdomyolysis attenuates activity of semicarbazide sensitive amine oxidase as the marker of nephropathy in diabetic rats

Semicarbazide diminishes the signs of bleomycin-induced pulmonary fibrosis in rats

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