Aminiotic Fluid and Intrauterine Growth Restriction in a Gastroschisis Fetal Rat Model

Franchi-Teixeira, Antonio Roberto; Barreto, Maria Weber Guimarães; Nogueira, Barbara; Bittencourt, Daniel Guimarães; Violin, Luis; Sbragia, Lourenço

doi:10.1159/000088037

Cited by 12 publications

(10 citation statements)

References 38 publications

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“…0.001). BW was not as altered in the subgroups (p = 0.793) as in the E18.5 and E19.5 groups (p = 0.271); these results are different from those of Albert et al [2] and FranchiTeixeira et al [27] who found a smaller BW in gastroschisis fetuses.…”

Section: Discussioncontrasting

confidence: 70%

“…0.001), but not between E18.5 and E19.5; mucosal thickening was also verified in lamb gastroschisis, with corresponding enlargement of the submucosa [4] . The same occurred in rabbit gastroschisis [27] and it seems that this thickening of the intestinal wall is related with a decrease in disaccharidase activity, contributing to neonatal intestinal malabsorption [17] .…”

Section: Discussionmentioning

confidence: 76%

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Maturity of the Myenteric Plexus Is Decreased in the Gastroschisis Rat Model

França¹,

Langone²,

Hoz³

et al. 2007

Fetal Diagn Ther

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Background: Amniotic fluid (AF) and its components, such as fetal urine and meconium, may lead to intestinal alterations in gastroschisis, which cause immaturity of the myenteric plexus and consequent intestinal hypomotility and malabsorption. In this study we identified morphological and histological alterations of the intestine and the myenteric plexus with two different times of exposure to AF. Methods: The experimental gastroschisis was achieved at two different gestational ages, on day 18.5 (E18.5) and day 19.5 (E19.5) of gestation, in fetal rats which were divided into 3 subgroups: control, sham and gastroschisis. We measured fetal body weight (BW), intestinal weight (IW) and intestinal length (IL). The layers of intestinal wall and myenteric plexus were evaluated by hematoxylin and eosin staining (HE staining) and immunofluorescence (α-internexin), respectively. Results: BW was not significantly different among the control, sham and gastroschisis groups at both ages. IW and IL were larger and shorter, respectively, in the gastroschisis fetuses (p < 0.001) at both ages. Intestinal diameters and wall layers presented significant differences among control, sham and gastroschisis fetuses at both ages (p < 0.001), but the time of exposure to AF compromised the serous membrane, D-II (diameter II, p < 0.001) and IL (p = 0.001). α-Internexin presented more intensive immunoreactivity in gastroschisis fetuses at E18.5. Conclusions: In gastroschisis, the longer the time of exposure to AF, the more severe bowel impairment will be, especially with regard to IL and the serous layer, and the more immature the myenteric plexus will be.

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Section: Discussioncontrasting

confidence: 70%

Section: Discussionmentioning

confidence: 76%

Maturity of the Myenteric Plexus Is Decreased in the Gastroschisis Rat Model

França¹,

Langone²,

Hoz³

et al. 2007

Fetal Diagn Ther

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“…These studies support the hypothesis that in utero protein and fluid loss from the bowel to the amniotic fluid may account for the raised incidence of growth restriction, oligohydramnios, and intrauterine death that occur in these at-risk pregnancies. A rat model has shown that gastroschisis had a direct correlation with IUGR, but that the time of exposure to the amniotic fluid had no influence on body weight, but did interfere with intestinal length [33] .…”

Section: Discussionmentioning

confidence: 99%

Gastroschisis: Growth Patterns and a Proposed Prenatal Surveillance Protocol

et al. 2007

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Objective: To assess intrauterine growth for fetuses with gastroschisis using retrospective serial ultrasound assessment from fetuses diagnosed prenatally with gastroschisis. The growth assessment could be available as a prospective tool to direct an antepartum fetal surveillance protocol. Methods: This is a retrospective review of all cases of gastroschisis evaluated prenatally at a single institution between February 1996 and March 2002. Charts were reviewed for serial ultrasound assessment, gestational age at delivery, mode of delivery, and birth weight. Growth assessment was determined for abdominal circumference, biparietal diameter, head circumference, femur length, and estimated fetal weight (IRB No. 2002-1-2648). Results: Forty patients had delivered by March 2002. One hundred and two ultrasound reports were reviewed. Gastroschisis growth curves showed that the 50th percentile was shifted to the right when compared to normal growth curves for abdominal cicumference, biparietal diameter, head circumference, and femur length. The average birth weight was 2,359 g. Compared with a standard population, 44% (16/36) were below the 5th percentile, 61% (22/36) were below the 10th percentile, and 95% (34/36) were below the 50th percentile for gestational age. The average gestational age at delivery was 36.3 weeks. Mothers were nulliparous in 78%, with a mean age of 21.3 years. Conclusions: (1) Fetuses with gastroschisis show a symmetric intrauterine growth restriction pattern consistent with early development of growth delay; (2) the 50th percentile biometry measurements for the gastroschisis population are shifted to the right on normal fetal growth curves; (3) the birth weight is at or below the 10th percentile in 61% of the newborns with gastroschisis, and (4) an antepartum surveillance protocol is proposed based on growth patterns of fetuses with gastroschisis.

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“…The reasons for this discrepancy include concerns about fetal growth and liquor volume as well as progression of bowel dilatation evident on ultrasound which prompted earlier delivery. There is certainly both human and animal evidence that fetal growth restriction occurs in gastroschisis [10,11] .…”

Section: Discussionmentioning

confidence: 99%

Outcome of Antenatally Diagnosed Fetal Anterior Abdominal Wall Defects from a Single Tertiary Centre

et al. 2008

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Objective: The aim of this study was to review the outcome of all cases of antenatally diagnosed anterior abdominal wall defects at a single tertiary centre. Method: 41 cases from the database of the Centre of Fetal Care at Queen Charlotte’s and Chelsea Hospital in London from 2000 to 2005 were reviewed and both obstetric and neonatal data were collected. Results: 25 cases were exomphalos (61%), 9 were gastroschisis (22%), 6 were body stalk anomaly (15%) and 1 case was cloacal exstrophy (2%). 17 cases (41%) were associated with other major malformations and 4 (10%) were aneuploid. There was 1 case of intrauterine death (2%). Termination of pregnancy was performed in 24 cases (63%). Of the cases that continued (exomphalos and gastroschisis), all babies survived surgery and were discharged home. Conclusions: This study demonstrates a high termination rate for fetuses diagnosed with anterior abdominal wall defects. However, the surgical outcome for euploid neonates with isolated exomphalos or gastroschisis appears to be good. Babies with gastroschisis required a longer period of parenteral feeding compared with babies with exomphalos. These infants however had a longer duration of hospitalization.

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Aminiotic Fluid and Intrauterine Growth Restriction in a Gastroschisis Fetal Rat Model

Cited by 12 publications

References 38 publications

Maturity of the Myenteric Plexus Is Decreased in the Gastroschisis Rat Model

Maturity of the Myenteric Plexus Is Decreased in the Gastroschisis Rat Model

Gastroschisis: Growth Patterns and a Proposed Prenatal Surveillance Protocol

Outcome of Antenatally Diagnosed Fetal Anterior Abdominal Wall Defects from a Single Tertiary Centre

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