AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT
            <i>SACCHAROMYCES CEREVISIAE</i>

Sorsoli, W. A.; Spence, Kemet D.; Parks, Leo W.

doi:10.1128/jb.88.1.20-24.1964

Cited by 39 publications

(19 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Demain (1964) suggested that a site of permeation may be the point of inhibition by normal purines of a guanineless mutant of Bacillus subtilis. Permeability has also been suggested for the basis of resistance of mutants of Saccharomyces cerevisiae to ethionine (Sorsoli, Spence, and Parks, 1964). There are other mechanisms that can also explain inhibition of growth by normal purines and resistance to amino acid analogues (Fangman and Neidhardt, 1964a, b;Lewis, 1963;Martin and Moo-Penn, 1963;Moyed, 1964;Rowbury and Woods, 1961).…”

Section: Discussionmentioning

confidence: 99%

Enhancement by Adenine of the Inhibition of Salmonella typhimurium by Ethionine

Smith

Salmon

1965

J Bacteriol

View full text Add to dashboard Cite

The inhibition by ethionine of the growth of Salmonella typhimurium was increased by adenine and several other purines. This effect was counteracted by the addition of methionine to the cultures. There was no preferential inhibition of nucleic acid or protein synthesis by adenine plus ethionine. Adenine stimulated the uptake of radioactive ethionine by the cells. Adenine may produce the effects observed by increasing the rate at which ethionine enters the cell and reaches its primary site of antibacterial action.

show abstract

Section: Discussionmentioning

confidence: 99%

Enhancement by Adenine of the Inhibition of Salmonella typhimurium by Ethionine

Smith

Salmon

1965

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Methionine assay. The uptake and intracellular concentration of methionine was determined by microbiological assay with Streptococcus faecalis strain 9790 as described earlier (13). The medium was assayed following removal of the cells by membrane filtration (Millipore).…”

Section: Methodsmentioning

confidence: 99%

sai-1 Mutation in Saccharomyces cerevisiae : Characteristics of Inhibition by S -Adenosylmethionine and S -Adenosylhomocysteine and Protection by Methionine

1972

View full text Add to dashboard Cite

The relationship of methionine to the inhibition caused by S-adenosylmethionine and S-adenosylhomocysteine in strains containing the sai-1 mutation has been investigated and shown to affect indirectly the survival of the mutants. The ability of the mutants to take up both inhibitors is similar to that of the wild-type cells. The mutant also retains the ability to hydrolyze S-adenosylhomocysteine and incorporate the hydrolytic products into the various cellular fractions. Maximal inhibition of the sai-I mutants occurs at an extracellular concentration of 0.005 mm S-adenosylmethionine and 0.025 to 0.05 mm Sadenosylhomocysteine when the cellular concentration is 0.05 mg (dry weight) per ml. The results suggest that the sai-1 mutation affects reaction(s) either not associated with methionine biosynthesis, or methionine synthesis and at least one other critical cellular function. 1050

show abstract

“…Genetic techniques. Methods leading to tetrad analysis, including mating, diploid isolation, sporulation, and genetic manipulation, were the same as those described by Sorsoli et al (5). For random analysis, the techniques above were employed except that, immediately after treatment of the sporulated diploid with snail enzyme, the ascospores were spread on the surface of TGY (1% tryptone, 2% glucose, 1% yeast extract) agar plates.…”

Section: Methodsmentioning

confidence: 99%

Mutation of Saccharomyces cerevisiae Preventing Uptake of S -Adenosylmethionine

Spence

1971

J Bacteriol

View full text Add to dashboard Cite

A mutant has been isolated whose aberration severely restricts the ability of cells of Saccharomyces cerevisiae to take up S-adenosylmethionine. The mutation apparently also affects adenosylhomocysteine uptake, but not that of the S-adenosylmethionine moieties adenine, homocysteine, homoserine, or methionine, nor the sulfonium compound, S-methylmethionine. It is a single, chromosomal mutation whose expression is not dependent on the presence of ammonium ions. 328 SPENCE on August 2, 2020 by guest

show abstract

AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE

Cited by 39 publications

References 15 publications

Enhancement by Adenine of the Inhibition of Salmonella typhimurium by Ethionine

Enhancement by Adenine of the Inhibition of Salmonella typhimurium by Ethionine

sai-1 Mutation in Saccharomyces cerevisiae : Characteristics of Inhibition by S -Adenosylmethionine and S -Adenosylhomocysteine and Protection by Methionine

Mutation of Saccharomyces cerevisiae Preventing Uptake of S -Adenosylmethionine

Contact Info

Product

Resources

About