Amino acid activation in hog pancreas

Schweet, Richard S.; Holley, Robert W.; Allen, Esther H.

doi:10.1016/0003-9861(57)90040-1

Cited by 47 publications

(10 citation statements)

References 11 publications

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“…animal tissues (Schweet et al 1957;Hoagland et al 1956;Davie et al 1956;Lipmann, 1958) and plant material (Davis & Novelli, 1958;Marcus, 1959;Clark, 1958;Webster, 1957). EXPERIMENTAL Material8 D-Alanine, D-oc-amino-n-butyric acid and D-serine were purchased from the California Corp. for Biochemical Research.…”

Section: Mg2+ Ionmentioning

confidence: 99%

“…In every case activity towards L-alarine was either absent or very low. Schweet et al (1957) noted that histidine, methionine, serine and valine are activated in extracts of Lactobacillu8 plantarum. In addition to D-and L-alanine, these amino acids were also activated in the hydroxamate assay by unpurified enzyme preparations from L. arabino8us.…”

Section: Distribution Of Activating Enzyme In Various Bacteriamentioning

confidence: 99%

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The enzymic activation of d-alanine

Baddiley

Neuhaus

1960

Biochemical Journal

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Section: Mg2+ Ionmentioning

confidence: 99%

Section: Distribution Of Activating Enzyme In Various Bacteriamentioning

confidence: 99%

The enzymic activation of d-alanine

Baddiley

Neuhaus

1960

Biochemical Journal

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“…Fluoride has been used to inhibit the interfering enzymes (Hoagland, 1955), but this may have only partial success because fluoride, above a certain concentration, also inhibits the activating enzymes (Davis & Novelli, 1958). Other workers found little inhibition of endogenous pyrophosphate-ATP exchange after the addition of fluoride (Schweet et al 1957), and they removed the interfering enzymes by adsorption on charcoal. The use of the hydroxamate technique is therefore recommended when the activation of amino acids is to be studied in tissues where phosphatases are known to be present.…”

Section: Discussionmentioning

confidence: 98%

“…Many workers have observed a high endogenous pyrophosphate-ATP exchange in the absence of added amino acids (Schweet, Holley & Allen, 1957;Novelli, 1958), and measurement of amino acidactivation rates by the exchange assay usually gave higher results than does the hydroxamate method (de Moss & Novelli, 1955;Novelli, 1958;Schweet & Allen, 1958). This is probably caused by the presence of pyrophosphatases, phosphokinases or other phosphatases in the enzyme extracts, and some care is required in the interpretation of observations if the exchange assay is used to measure amino acid activation in these preparations.…”

Section: Discussionmentioning

confidence: 99%

The Effect of X-Irradiation on the Activation of Amino Acids in Nuclei From Rat Thymus Gland

Ja¹,

La²

1963

Biochemical Journal

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“…As is known, these enzymes are located in the microsomal supernatant and are assumed to be involved in the initial steps of the process of protein synthesis (22)(23)(24)(25)(26)(27)(28).…”

mentioning

confidence: 99%

A Cytochemical Study on the Pancreas of the Guinea Pig

Siekevitz

Palade

1959

The Journal of Cell Biology

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Five ribonucteoprotein (RNP) fractions were isolated from the postmitochondrial supernatant of the pancreas of the guinea pig. Two were obtained from the microsomes which, by deoxycholate (DOC) treatment, were subdivided into a DOC-soluble and a DOC-insoluble fraction. The latter was taken to represent attached RNP particles. Two other fractions obtained from the microsomal supernatant supposedly represent free RNP particles existing as such in the cytoplasm, while a third fraction resisted sedimentation for 20 hours at 105,000 g and is considered to be a soluble nucleoprotein. These fractions exhibited different RNA/ protein ratios and also different RNA turnover patterns, as determined after in vivo labelling with adenine-8-C 14. However, little discernible differences could be detected in the nucleotide composition of the RNA moieties of these RNP fractions.Amino acid-"activating" enzymes were found to occur in the fraction containing the soluble nucleoproteins.The discussion focuses on the relationships between these fractions and protein synthesis in the pancreas, using data given in this and a previous paper, and data contained in the literature.In a detailed cytochemical investigation of the pancreas of the guinea pig (1-5), we have found that it is possible to separate several cytologically distinct types of nucleoprotein particles (2, 5) derived mostly from the cytoplasm of the exocrine cells of this organ. Since we have already studied (5) the in vivo turnover of the proteins of these particles, we have decided to determine, also in vivo, the turnover of their nucleic acid moiety.In this paper we present our findings on this point together with additional data pertaining to the chemical composition of the particles.The metabolic heterogeneity of the ribonudeic acid (RNA) at cellular and subcellular levels has

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Amino acid activation in hog pancreas

Cited by 47 publications

References 11 publications

The enzymic activation of d-alanine

The enzymic activation of d-alanine

The Effect of X-Irradiation on the Activation of Amino Acids in Nuclei From Rat Thymus Gland

A Cytochemical Study on the Pancreas of the Guinea Pig

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