Amino Acid Activation in the Liver of Growing Rats Maintained with Normal and with Protein-deficient Diets

Mariani, A.; Migliaccio, P. A.; Spadoni, M. A.; Ticca, M.

doi:10.1093/jn/90.1.25

Cited by 23 publications

(10 citation statements)

References 14 publications

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“…Group C has the highest growth rate, followed by groups ADP, AS, AD and G, in that order. Throughout the experimental period, the weights of the four groups under continuous or alternate supplementation fell within the range of values usually encountered in rats in our colony of the same age and stock and maintained on balanced and complete diets [7], Time in days The weight gain of non-supplemented group G appears significantly inferior (p < 0.05-0.001). However, contrary to what was observed at lower levels of protein content [4], this group was capable of a rather satisfactory growth rate.…”

Section: Resultssupporting

confidence: 51%

Studies on Intermittent Supplementation

Ferro-Luzzi¹,

Migliaccio²,

Sorrentino³

et al. 1972

Ann Nutr Metab

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A discontinuous casein supplementationat 24-hour intervals of an only-gluten diet was reported previously to result in an incomplete growth recovery, as well as a delayed liver-cell maturation when the protein level in the diet was 10%. When such a discontinuous supplementation was carried out at 14% protein level, the growth recovery was sufficient to ensure a practically normal terminal body weight; but a delay in liver-cell multiplication was found. Moreover, at both protein levels, the recovery rate on the day of supplementation appeared to depend on the level of nitrogen intake rather than on the balance existing between non-supplemented and supplemented diets. The effects of 24- and 48-hour intervals between successive casein supplements of a 30-percent only-gluten protein diet on growing rats have now been investigated. The results indicate that, at this artificially high protein level, the utilization of the dietary protein, as indicated by several criteria (PER, NGI, NPU), does not appear to be impaired by discontinuity in the supplementation, even when the intervals are as long as 48 h. The growth recovery is complete, and the carcass and liver composition values fall within the normal range. In the liver, at the cellular level, the results indicate a lack of interference with both the cell maturation and multiplication. In addition, the liver cell ability to ‘store’ nitrogen in high-protein diets appears unimpaired by discontinuity in supplementation under the conditions investigated in the present study.

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Section: Resultssupporting

confidence: 51%

Studies on Intermittent Supplementation

Ferro-Luzzi¹,

Migliaccio²,

Sorrentino³

et al. 1972

Ann Nutr Metab

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“…The remaining homogenate was used for the count of the nuclei in a Ilurker camera by a modified method of Price el al. [8][9][10][11][12]. At the same time, protein nitrogen was determined in wet liver by nessler method.…”

Section: Methodsmentioning

confidence: 99%

Studies on Intermittent Supplementation

Ferro-Luzzi¹,

Mariani²,

Migliaccio³

1970

Ann Nutr Metab

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Two lots of weanling Sprague-Dawley rats were fed, for 30 days, diets at 10 and 14% protein content. Each lot consisted of 4 groups fed on: (a) a gluten diet; (b) a gluten diet continuously supplemented with casein; (c) a gluten diet on odd days, and a gluten diet supplemented with casein, ad libitum, on even days; (d) a gluten diet on odd days and a pair-fed gluten diet supplemented with casein on even days. Growth-curves, PER, NPU, NGI, and carcass and liver composition have been studied. The results show that the recovery rate in the days of supplementation is dependent on the level of nitrogen intake rather than on the balance occurring between non-supplemented and supplemented diets, according to whether the latter is offered ad libitum or pair-fed. At the cellular level, the count of nuclei and the DNA-P/nucleus content in liver cells show that in groups at lower protein intake, the different ways of supplementation affect cellular maturation, while in groups with higher protein intake it is cellular multiplication that is affected.

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“…In contrast, Mariani et al (1966) reported data con sistent with ploidy increases at 10, 20, and 30 days in weanling control animals fed 25% casein. The increase was blocked when a low protein diet (5% casein) was fed.…”

Section: Patterns In Protein Deficiencymentioning

confidence: 82%

“…Variations in animal age do not reconcile the differences because in weanling animals, ploidy was reported to increase (Lecojnte and de Smul 1952), to be unaffected (Umana 1965), or to decrease relative to controls (Mariani et al 1966;Fukuda and Sibatani 1953b). Similarly, in older animals, the index of nuclear ploidy was reported to be unaffected (Thomson et al 1953) or to increase due to protein deficiency (Ely and Ross 1951;Umana 1965Umana , 1967.…”

Section: Patterns In Protein Deficiencymentioning

confidence: 99%

“…The present data do not address the situation in older animals in which average ploidy has been purported to increase (Umana 1965(Umana , 1967Ely and Ross 1951) or to be unchanged (Thomson et al 1953) due to the protein content of the diet. However, suppression of tetraploid cell formation has previously been postulated to occur when weanling animals were fed 5% casein (Mariani et al 1966) or diets otherwise limited in protein content ( Nadal and Zajdela 1965b).…”

Section: A Suppression Of Polyploidizationmentioning

confidence: 99%

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Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

Moore

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Amino Acid Activation in the Liver of Growing Rats Maintained with Normal and with Protein-deficient Diets

Cited by 23 publications

References 14 publications

Studies on Intermittent Supplementation

Studies on Intermittent Supplementation

Studies on Intermittent Supplementation

Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

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