Amino acid and N mineralization dynamics in heathland soil after long-term warming and repetitive drought

Andresen, Louise C.; Bodé, Samuel; Tietema, Albert; Boeckx, Pascal; Rütting, Tobias

doi:10.5194/soild-1-803-2014

Cited by 13 publications

(15 citation statements)

References 55 publications

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“…In contrast to our hypothesis, we did not find any significant effect of climatic treatments or vegetation type on gross N transformations (Supplementary Table 2). This is in line with the generally small changes in plant growth in the TasFACE experiment (Hovenden et al 2014), which did consequently not Similar to our results, gross N mineralization was unaffected by warming in a Dutch heathland (Andresen et al 2015). On the other hand, both increased (Larsen et al 2011;Björsne et al 2014) and decreased (Jamieson et al 1998;Niboyet et al 2011) gross N mineralization under warming has been reported.…”

Section: Resultssupporting

confidence: 90%

Soil nitrogen cycle unresponsive to decadal long climate change in a Tasmanian grassland

Rütting

Hovenden

2019

Biogeochemistry

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Increases in atmospheric carbon dioxide (CO 2) and global air temperature affect all terrestrial ecosystems and often lead to enhanced ecosystem productivity, which in turn dampens the rise in atmospheric CO 2 by removing CO 2 from the atmosphere. As most terrestrial ecosystems are limited in their productivity by the availability of nitrogen (N), there is concern about the persistence of this terrestrial carbon sink, as these ecosystems might develop a progressive N limitation (PNL). An increase in the gross soil N turnover may alleviate PNL, as more mineral N is made available for plant uptake. So far, climate change experiments have mainly manipulated one climatic factor only, but there is evidence that single-factor experiments usually overestimate the effects of climate change on terrestrial ecosystems. In this study, we investigated how simultaneous, decadal-long increases in CO 2 and temperature affect the soil gross N dynamics in a native Tasmanian grassland under C3 and C4 vegetation. Our laboratory 15 N labeling experiment showed that average gross N mineralization ranged from 4.9 to 11.3 lg N g-1 day-1 across the treatment combinations, while gross nitrification was about ten-times lower. Considering all treatment combinations, no significant effect of climatic treatments or vegetation type (C3 versus C4 grasses) on soil N cycling was observed.

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Section: Resultssupporting

confidence: 90%

Soil nitrogen cycle unresponsive to decadal long climate change in a Tasmanian grassland

Rütting

Hovenden

2019

Biogeochemistry

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“…Therefore, 1 mL deionized water was slowly added dropwise to each soil one day before labelling. The two 15 N labels were: 1. a 20 amino acids-mixture (AA; 'cell free powder' from Cambridge isotope laboratories; see (Andresen et al 2015)) with 99% 15 N, dissolved in 0.1 MolÁL -1 HCl, containing 0.36 g AA L -1 corresponding to 3 lg AA-N g -1 soil. ; and 2. ammonium sulphate with 99.8% 15 N, amended at a rate of 2 lg N g -1 soil.…”

Section: Nitrogen Turnover Rates By Pool Dilution Techniquementioning

confidence: 99%

“…The method to assess the individual free amino acid (FAA) content and isotopic enrichment is described in detail elsewhere Andresen et al 2015Andresen et al , 2016a. In short, at arrival at ISOFYS lab, the SPE columns were washed with 10 mL of ultrapure water after which the FAAs were eluted with 30 mL 3 MolÁL -1 NH 4 OH.…”

Section: Abundance and Isotopic Composition Of Individual Amino Acid mentioning

confidence: 99%

Nitrogen dynamics after two years of elevated CO2 in phosphorus limited Eucalyptus woodland

et al. 2020

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It is uncertain how the predicted further rise of atmospheric carbon dioxide (CO2) concentration will affect plant nutrient availability in the future through indirect effects on the gross rates of nitrogen (N) mineralization (production of ammonium) and depolymerization (production of free amino acids) in soil. The response of soil nutrient availability to increasing atmospheric CO2 is particularly important for nutrient poor ecosystems. Within a FACE (Free-Air Carbon dioxide Enrichment) experiment in a native, nutrient poor Eucalyptus woodland (EucFACE) with low soil organic matter (≤ 3%), our results suggested there was no shortage of N. Despite this, microbial N use efficiency was high (c. 90%). The free amino acid (FAA) pool had a fast turnover time (4 h) compared to that of ammonium (NH4+) which was 11 h. Both NH4-N and FAA-N were important N pools; however, protein depolymerization rate was three times faster than gross N mineralization rates, indicating that organic N is directly important in the internal ecosystem N cycle. Hence, the depolymerization was the major provider of plant available N, while the gross N mineralization rate was the constraining factor for inorganic N. After two years of elevated CO2, no major effects on the pools and rates of the soil N cycle were found in spring (November) or at the end of summer (March). The limited response of N pools or N transformation rates to elevated CO2 suggest that N availability was not the limiting factor behind the lack of plant growth response to elevated CO2, previously observed at the site.

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“…Depolymerization of proteinaceous organic material is an important pathway for generating bioavailable N in wide range of systems including boreal forests and is often considered a fungal trait (5, 7, 20, 22, 26-28). Depolymerization decomposes polymeric organic material into monomers and amino acids that can be used as C and N sources by soil microorganisms and plants (19, 20, 22, 29, 30).…”

Section: Introductionmentioning

confidence: 99%

High Genetic Potential for Proteolytic Decomposition in Northern Peatland Ecosystems

Graham

Yang²,

Bell

et al. 2018

Preprint

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33Nitrogen (N) is a scarce nutrient commonly limiting primary productivity. Microbial 34 decomposition of complex carbon (C) into small organic molecules (e.g., free amino acids) has 35 been suggested to supplement biologically-fixed N in high latitude peatlands. We evaluated the 36 microbial (fungal, bacterial, and archaeal) genetic potential for organic N depolymerization in 37 peatlands at Marcell Experimental Forest (MEF) in northern Minnesota. We used guided gene 38 assembly to examine the abundance and diversity of protease genes; and further compared to 39 those of N-fixing (nifH) genes in shotgun metagenomic data collected across depth at two 40 distinct peatland environments (bogs and fens). Microbial proteases greatly outnumbered nifH 41 genes with the most abundant gene families (archaeal M1 and bacterial Trypsin) each containing 42 more sequences than all sequences attributed to nifH. Bacterial protease gene assemblies were 43 diverse and abundant across depth profiles, indicating a role for bacteria in releasing free amino 44 acids from peptides through depolymerization of older organic material and contrasting the 45 paradigm of fungal dominance in depolymerization in forest soils. Although protease gene 46 assemblies for fungi were much less abundant overall than for bacteria, fungi were prevalent in 47 surface samples and therefore may be vital in degrading large soil polymers from fresh plant 48 inputs during early stage of depolymerization. In total, we demonstrate that depolymerization 49 enzymes from a diverse suite of microorganisms, including understudied bacterial and archaeal 50 lineages, are likely to play a substantial role in C and N cycling within northern peatlands. 51 52

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Amino acid and N mineralization dynamics in heathland soil after long-term warming and repetitive drought

Cited by 13 publications

References 55 publications

Soil nitrogen cycle unresponsive to decadal long climate change in a Tasmanian grassland

Soil nitrogen cycle unresponsive to decadal long climate change in a Tasmanian grassland

Nitrogen dynamics after two years of elevated CO2 in phosphorus limited Eucalyptus woodland

High Genetic Potential for Proteolytic Decomposition in Northern Peatland Ecosystems

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