Amino Acid‐Based Dendrimers

Shi, Zhou; Zhou, Chunhui; Liu, Zhigang; Totsingan, Filbert; Kallenbach, Neville R.

doi:10.1002/9783527631827.ch15

Cited by 2 publications

(2 citation statements)

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“…The presence of bifunctional N α -and N ε -amino groups in each lysine served as reactive ends for the conjugation. 49 In amino groups of N ε -terminal of lysine residues, 4-pentynoic acids were coupled to introduce the alkyne functionalities whereas, amino acids of PADRE sequence and acyl group were sequentially coupled to N α -terminal sites of lysine residues. Azido-functionalized J8 peptide (QAEDKVKQSREAKKQ-VEKALKQLEDKVQ) was also synthesized using the same technique.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…This process was continued to conjugate immunosilent two-branched lysine dendrimer. The presence of bifunctional N α - and N ε -amino groups in each lysine served as reactive ends for the conjugation . In amino groups of N ε -terminal of lysine residues, 4-pentynoic acids were coupled to introduce the alkyne functionalities whereas, amino acids of PADRE sequence and acyl group were sequentially coupled to N α -terminal sites of lysine residues.…”

Section: Results and Discussionmentioning

confidence: 99%

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Immunogenicity Assessment of Cell Wall Carbohydrates of Group A Streptococcus via Self-Adjuvanted Glyco-lipopeptides

et al. 2021

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Identifying the immunogenic moieties and their precise structure of carbohydrates plays an important role for developing effective carbohydrate-based subunit vaccines. This study assessed the structure–immunogenicity relationship of carbohydrate moieties of a single repeating unit of group A carbohydrate (GAC) present on the cell wall of group A Streptococcus (GAS) using a rationally designed self-adjuvanted lipid-core peptide, instead of a carrier protein. Immunological evaluation of fully synthetic glyco-lipopeptides (particle size: 300–500 nm) revealed that construct consisting of higher rhamnose moieties (trirhamnosyl-lipopeptide) was able to induce enhanced immunogenic activity in mice, and GlcNAc moiety was not found to be an essential component of immunogenic GAC mimicked epitope. Trirhamnosyl-lipopeptide also showed 75–97% opsonic activity against four different clinical isolates of GAS and was comparable to a subunit peptide vaccine (J8-lipopeptide) which illustrated 65–96% opsonic activity.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%