Amino Acid-Derived Quorum Sensing Molecule Alanine on the Gastrointestinal Tract Tolerance of the
            <i>Lactobacillus</i>
            Strains in the Cocultured Fermentation Model

Wen, Jianzhu; Yu, Yixing; Chen, Mengqi; Cui, Lei; Xia, Qiang; Zeng, Xiaoqun; Guo, Yuxing; Wu, Zhen

doi:10.1128/spectrum.00832-21

Cited by 12 publications

(8 citation statements)

References 38 publications

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“…Plates were incubated in reach-in plant growth incubators (Conviron Adaptis 1000, Canada) at 25 ± 0.2 ᴼC, 75% RH, 16h Light/8h Dark, and 100 µmoles/m 2 /s light intensity. After two weeks, six (6) uniformly growing seedlings were transferred to each of 3 to 7 plates containing 3MM paper wetted with 5 mL half-strength MS without sucrose. Plates were horizontally incubated for one day to allow seedlings to stabilize.…”

Section: Methodsmentioning

confidence: 99%

“…After centrifugation for 5 min at 10,000g, the 3 supernatants from each treatment and control condition were pooled (4) and filtered-sterilized (5) . Aliquots of the supernatants were used to quantify ammonia (6) using the ammonia assay kit from AbCam (Cat # ab102509), according to the manufacturer’s guidelines. The experiment was repeated 3 times with independent batches of fresh exudates.…”

Section: Figure S1mentioning

confidence: 99%

“…Seedings were grown on vertical plates as described above. Two weeks after sowing, six (6) uniformly growing seedlings were transferred to each of 5 plates containing 3MM paper wetted with 5 mL half-strength MS without sucrose. Plates were horizontally incubated for one day to allow seedlings to attach to the paper surface.…”

Section: Root Colonizationmentioning

confidence: 99%

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The Arabidopsis LHT1 amino acid transporter contributes toPseudomonas simiae-mediated plant-growth promotion by modulating bacterial metabolism in the rhizosphere

Agorsor

Kagel

Danna

2022

Preprint

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Belowground microbiome structure ensures optimal plant-host health and fitness, and it is, at least in part, defined by the plant genotype. Root-secreted amino acids (AAs) promote microbial chemotaxis and growth in the rhizosphere. However, whether the plant-mediated reuptake of AAs contributes to maintaining optimal levels of AAs in the rhizosphere, and in turn, microbial growth, remains to be established. The Arabidopsis thaliana LHT1 is a high-affinity transporter expressed in the rhizodermis that can take up AAs from the soil. Here we show that a loss-of-function mutant for LHT1 leads to increased accumulation of plant-derived amino acids in the root exudates and that lht1 root exudates enable more growth of the plant-growth-promoting bacteria (PGPB) Pseudomonas simiae WCS417r (Ps WCS417r). Amino acid profiling of the lht1 exudates showed increased levels of glutamine (Gln) and other amino acids. Excitingly, supplementing wild-type root exudates with Gln was sufficient to promote Ps WCS417r growth. However, intriguingly, despite promoting Ps WCS417r growth, lht1 exudates and wild-type exudates supplemented with Gln inhibit plant growth in a Ps WCS417r-dependent manner. These data suggest that an excess of AAs in the rhizosphere impacts Ps WCS417r metabolism which in turn inhibits plant growth. Together, the results unveil that LHT1 regulates the amino acid-mediated interaction between plants and Ps WCS417r and suggest a complex relationship between root-exuded metabolites, PGPB, and plant growth.

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Section: Methodsmentioning

confidence: 99%

Section: Figure S1mentioning

confidence: 99%

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The Arabidopsis LHT1 amino acid transporter contributes toPseudomonas simiae-mediated plant-growth promotion by modulating bacterial metabolism in the rhizosphere

Agorsor

Kagel

Danna

2022

Preprint

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“…However, S. thermophilus has been found to exist in multispecies biofilms born by wood vats used for traditional fermentation (Settanni et al., 2012). Another example is the coculture biofilm formed by S. thermophilus , Lactobacillus bulgaricus , and L. acidophilus in a 1:1:1 ratio, which was significantly promoted compared to L. acidophilus monoculture (J. Wen et al., 2022). During food production, L. monocytogenes is a poor biofilm former that can produce thin biofilms by itself and is even unable to form biofilms in the presence of shear forces (Fagerlund et al., 2020).…”

Section: Formation Of Multispecies Biofilmsmentioning

confidence: 99%

Multispecies biofilms in fermentation: Biofilm formation, microbial interactions, and communication

Yao

Hao

Zhou

et al. 2022

Comp Rev Food Sci Food Safe

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Food fermentation is driven by microorganisms, which usually coexist as multispecies biofilms. The activities and interactions of functional microorganisms and pathogenic bacteria in biofilms have important implications for the quality and safety of fermented foods. It was verified that the biofilm lifestyle benefited the fitness of microorganisms in harsh environments and intensified the cooperation and competition between biofilm members. This review focuses on multispecies biofilm formation, microbial interactions and communication in biofilms, and the application of multispecies biofilms in food fermentation. Microbial aggregation and adhesion are important steps in the early stage of multispecies biofilm formation. Different biofilm-forming abilities and strategies among microorganisms lead to several types of multispecies biofilm formation. The spatial distribution of multispecies biofilms reflects microbial interactions and biofilm function. Then, we discuss the intrinsic factors and external manifestations of multispecies biofilm system succession. Several typical interspecies cooperation and competition modes and mechanisms of microbial communication were reviewed in this review. The main limitations of the studies included in this review are the relatively small number of studies of biofilms formed by functional microorganisms during fermentation and the lack of direct evidence for the formation process of multispecies biofilms and microbial interactions and communication within biofilms. This review aims to provide the food industry with a sufficient understanding of multispecies biofilms in food fermentation.

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“…These fluids, known as root exudates, influence the rhizosphere by inhibiting the growth of harmful microbes while promoting the growth of beneficial ones. Amino acids (AAs) are among the most represented metabolites in the root exudates of the model plant Arabidopsis thaliana [ 2 , 3 , 4 ] Microbes in the rhizosphere use AAs, or their derivatives, as food sources and perhaps to communicate with other microbes (e.g., quorum sensing) [ 5 , 6 , 7 ] In addition, microbes use AA concentration gradients to navigate the soil toward roots. For example, in the colonization of tomato root tips, wild-type Pseudomonas fluorescens Pf0–1 outcompetes a triple-mutant derivative strain with impaired chemotaxis towards AAs [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis LHT1 Amino Acid Transporter Contributes to Pseudomonas simiae-Mediated Plant Growth Promotion by Modulating Bacterial Metabolism in the Rhizosphere

Agorsor

Kagel

Danna

2023

Plants

View full text Add to dashboard Cite

The root microbiome structure ensures optimal plant host health and fitness, and it is, at least in part, defined by the plant genotype. It is well documented that root-secreted amino acids promote microbial chemotaxis and growth in the rhizosphere. However, whether the plant-mediated re-uptake of amino acids contributes to maintaining optimal levels of amino acids in the root exudates, and, in turn, microbial growth and metabolism, remains to be established. Here, we show that Lysine-Histidine Transporter-1 (LHT1), an amino acid inward transporter expressed in Arabidopsis thaliana roots, limits the growth of the plant-growth-promoting bacteria Pseudomonas simiae WCS417r (Ps WCS417r). The amino acid profiling of the lht1 mutant root exudates showed increased levels of glutamine, among other amino acids. Interestingly, lht1 exudates or Gln-supplemented wild-type exudates enhance Ps WCS417r growth. However, despite promoting bacterial growth and robust root colonization, lht1 exudates and Gln-supplemented wild-type exudates inhibited plant growth in a Ps WCS417r-dependent manner. The transcriptional analysis of defense and growth marker genes revealed that plant growth inhibition was not linked to the elicitation of plant defense but likely to the impact of Ps WCS417r amino acids metabolism on auxin signaling. These data suggest that an excess of amino acids in the rhizosphere impacts Ps WCS417r metabolism, which, in turn, inhibits plant growth. Together, these results show that LHT1 regulates the amino-acid-mediated interaction between plants and Ps WCS417r and suggest a complex relationship between root-exuded amino acids, root colonization by beneficial bacteria, bacterial metabolism, and plant growth promotion.

show abstract

Amino Acid-Derived Quorum Sensing Molecule Alanine on the Gastrointestinal Tract Tolerance of the Lactobacillus Strains in the Cocultured Fermentation Model

Cited by 12 publications

References 38 publications

The Arabidopsis LHT1 amino acid transporter contributes toPseudomonas simiae-mediated plant-growth promotion by modulating bacterial metabolism in the rhizosphere

The Arabidopsis LHT1 amino acid transporter contributes toPseudomonas simiae-mediated plant-growth promotion by modulating bacterial metabolism in the rhizosphere

Multispecies biofilms in fermentation: Biofilm formation, microbial interactions, and communication

The Arabidopsis LHT1 Amino Acid Transporter Contributes to Pseudomonas simiae-Mediated Plant Growth Promotion by Modulating Bacterial Metabolism in the Rhizosphere

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