Amino acid HPLC-FLD analysis of spirulina and its protective mechanism against the combination of obesity and colitis in wistar rats

Arrari, Fatma; Jabri, Mohamed-Amine; Ayari, Ala; Dakhli, Nouha; Ben Fayala, Chayma; Boubaker, Samir; Sebai, Hichem

doi:10.1016/j.heliyon.2024.e30103

Heliyon

2024

DOI: 10.1016/j.heliyon.2024.e30103

|View full text |Cite

Amino acid HPLC-FLD analysis of spirulina and its protective mechanism against the combination of obesity and colitis in wistar rats

Fatma Arrari,

Mohamed-Amine Jabri,

Ala Ayari

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

2025

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 116 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Spirulina platensis: Unveiling phenotypic plasticity impact on its metabolic profile and bioactivity via chemometric analysis

Emam,

Abdel-Sattar,

Salama

et al. 2025

Food Bioscience

View full text Add to dashboard Cite

Spirulina platensis: Unveiling phenotypic plasticity impact on its metabolic profile and bioactivity via chemometric analysis

Emam,

Abdel-Sattar,

Salama

et al. 2025

Food Bioscience

View full text Add to dashboard Cite

Protective Effects of Spirulina Against Lipid Micelles and Lipopolysaccharide-Induced Intestinal Epithelium Disruption in Caco-2 Cells: In Silico Molecular Docking Analysis of Phycocyanobilin

Arrari,

Ortiz-Flores,

Lhamyani

et al. 2024

Nutrients

View full text Add to dashboard Cite

Damage to intestinal epithelial cells is present in obesity and other diseases because of inflammatory and oxidative processes. This damage compromises the gastrointestinal barrier, killing enterocytes, altering intestinal permeability, and eliciting abnormal immune responses that promote chronic inflammation. Recent evidence shows that spirulina is a potent natural agent with antioxidant and anti-inflammatory properties. Objectives: This study was conducted to evaluate the effect of spirulina aqueous extract (SPAE) on the alterations of the intestinal epithelium induced by lipid micelles (LMs) and/or inflammation induced by lipopolysaccharides (LPSs) in the Caco-2 cell line. Methods: In the current research, we assessed the protective actions of SPAE against cytotoxicity, oxidative stress, inflammation, and epithelial barrier perturbation by using an in vitro model, the intestinal Caco-2 cells, treated with LPSs and/or LMs. We also performed an in silico molecular docking analysis with spirulina’s bioactive compound, phycocyanobilin. Results: Our results showed that SPAE has no cytotoxic effect on Caco-2 cells. On the contrary, it improved cell viability and exhibited anti-inflammatory and antioxidant actions. SPAE also protected against endoplasmic reticulum stress and tight junction proteins, thus improving the epithelial barrier. The in silico study revealed a strong binding affinity of the phycocyanobilin compound with human SOD and NADPH oxidase and a good binding affinity towards COX-2 and iNOS. Conclusions: Taken together, these findings demonstrate the beneficial actions of SPAE on Caco-2 cells, suggesting it may be useful in preserving the epithelial intestinal barrier in human conditions involving oxidative stress and inflammation such as obesity.

show abstract

Nutritional Composition and Functional Properties of A. platensis-Derived Peptides: A Green and Sustainable Protein-Rich Supplement

Begum,

Qi,

Yang

et al. 2024

Processes

View full text Add to dashboard Cite

Among cyanobacterium, Arthrospira platensis (A. platensis) is a rich source of diverse bioactive compounds due to its high protein, essential amino acid, vitamin, and mineral content. A. platensis is one of the most abundant sources of protein (50–70%). In the food industry, A. platensis is being used as an ingredient for the development of food flavor, taste, and nutritional composition. Several in vitro and in vivo studies have revealed the potential use of A. platensis in the prevention and treatment of various metabolic diseases. Recently, extensive research has focused on the production and bioactivity of the A. platensis-derived bioactive peptides. A series of steps were used for the production of bioactive peptides including hydrolysis, ultrafiltration, and chromatographic techniques, coupled with an advanced detector. A. platensis peptides showed health benefits such as anti-hypertension, anti-diabetes, anti-microbial, antioxidant, anti-obesity, and anti-cancer activities. This review aims to present the main nutritional composition of A. platensis, the processes of purification, and the identification of bioactive peptides, and the potential health benefits such as antihypertensive, antidiabetic, anti-cancer, anti-obesity, antioxidant, and anti-microbial activities associated with the consumption of A. platensis-derived peptides are discussed. The originality of this review over the old review is that our review comprehensively studies the macro- and micronutrient composition and listed bioactive peptides to date, which can play an important role in the treatment of various diseases. Moreover, this review provides information related the research gaps of the various technologies that should be used for the development of the peptide as a pharmaceutical and functional food.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Amino acid HPLC-FLD analysis of spirulina and its protective mechanism against the combination of obesity and colitis in wistar rats

Cited by 3 publications

References 116 publications

Spirulina platensis: Unveiling phenotypic plasticity impact on its metabolic profile and bioactivity via chemometric analysis

Spirulina platensis: Unveiling phenotypic plasticity impact on its metabolic profile and bioactivity via chemometric analysis

Protective Effects of Spirulina Against Lipid Micelles and Lipopolysaccharide-Induced Intestinal Epithelium Disruption in Caco-2 Cells: In Silico Molecular Docking Analysis of Phycocyanobilin

Nutritional Composition and Functional Properties of A. platensis-Derived Peptides: A Green and Sustainable Protein-Rich Supplement

Contact Info

Product

Resources

About