Amino Acid Imprinted UiO-66s for Highly Recognized Adsorption of Small Angiotensin-Converting-Enzyme-Inhibitory Peptides

Abstract

Introduction of targeted defects into microporous UiO-66s for manipulating their three-dimensional size and surface properties can endow them with adsorption and separation areas involving angiotensin-converting-enzyme-inhibitory (ACE-inhibitory) peptides. Three hydrophobic amino acids (AAs) (i.e., proline (Pro), phenylalanine (Phe), and tryptophan (Trp)) having different physical/chemical properties were applied to in situ tailor defects in UiO-66 through targeted incoordination of missing linkers or missing nodes. Characterization results revealed a uniform oval shape of the developed defects with lengths ranging from 1.8 to 3.1 nm, which was also highly consistent with our molecular simulation. Among these three defective UiO-66s, Phe and Trp imprinted UiO-66s significantly promoted the adsorption affinity of small ACE-inhibitory peptides (uptake: 1.25 mmol g–1 for DDFF and 1.37 mmol g–1 for DDWW) and ultrahigh selectivity for DDFF (249) or DDWW (279) from inactive KKKK solution based on a lock-and-key mechanism. As a result, the imprinted UiO-66 showed an enrichment capacity for ACE-inhibitory peptides about eight times higher than that of pristine UiO-66. Therefore, the amino acid imprinting strategy endorsed by its facile and discerning ability can be envisioned to be of great value for small functional peptide separation and oriented enrichment in biomedicines.

Adsorptive, kinetics and regeneration studies of fluoride removal from water using zirconium-based metal organic frameworks

“…2f shows a lattice fringe spacing of 0.304 nm which corresponds to the (011) planes of ZrO 2 . 28 The average particle size of the MOF-801 obtained using soware ImageJ (random 50 particles) was 269.4 AE 2.97 nm.…”

Amino Acid Functionalized Crystalline Porous Polymers

“…通过控制反应 条件和配体结构, 氨基酸与有机配体比例最高可到 1∶ 1, 由此制备氨基酸功能化 MOF 材料. 2019 年, 赵钟兴 课题组 [34] 利用分子印迹策略, 选用 L-脯氨酸、L-苯丙氨 酸、L-色氨酸(L-Trp)三种疏水性的氨基酸在 UiO-66 合 成过程中原位构筑具有印迹的缺陷, 并成功将其孔道从 微孔扩大至介孔(3.1 nm). 在这一体系中, L-脯氨酸未能 成功引发缺陷, 猜测这与反应体系的化学环境、氨基酸 自身性质等有关.…”

“…Rosseinsky 等 [25] 利用三肽作为配体制备了三维柔性 MOF 材料 -ZnGGH(图 11a～11c), 基于短肽连接单元中共价键的 旋转, 这种 MOF 材料可以响应化学环境的变化诱导短 肽的构象发生改变, 在九个不同的晶体结构中灵活变 imprinted UiO-66s for highly recognized adsorption of small angiotensinconverting-enzyme-inhibitory peptides. Adapted from the literature [25], [34] 换, 从而触发 MOFs 对二氧六环、环戊烷、N,N-二甲基 甲酰胺(DMF)、呋喃以及二甲基亚砜(DMSO)等小分子 客体的吸附摄取.…”

“…框架材料中氨基酸、肽段的引入, 可促进多肽在孔 道中的选择性吸附和富集. 前文中已提到赵钟兴课题 组 [34] [39] 报道了一例谷胱甘肽功能化的磁性 COF 核壳结构微球-MCNC@COF@GSH, 由于尺寸筛分效 应和亲水的孔道环境, COF 微球可以高效富集 N-连接糖 肽, 表现出高选择性、高灵敏度、可循环使用性, 因而 有望应用于复杂生物样品的分析.…”

Porous Fe@C Composites Derived from Silkworm Excrement for Effective Separation of Anisole Compounds

“…Molecular imprinting and affinity separation have been used to recognize target molecules and realize great selectivity for larger biomolecules such as peptides and proteins. 18 However, separation of anisole or anisaldehyde from anise is still very challenging because ofo their similar molecular size, high boiling points, and diverse concentrations in industrial processes. As far as we are aware, there are rarely published reports about this separation.…”