Amino acid-modified chitosan nanoparticles for Cu<sup>2+</sup> chelation to suppress CuO nanoparticle cytotoxicity

Zhang, Yixian; Xu, Yiran; Xi, Xiangyi; Shrestha, Surakshya; Jiang, Peipei; Zhang, Wenjing; Gao, Changyou

doi:10.1039/c7tb00344g

Cited by 16 publications

(11 citation statements)

References 65 publications

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“…In NPs-based drugs discovery, for example, confirmation of NPs potency and their side effects in vitro are important, because in vitro experiments can sometimes provide potential outcomes with drug candidate agents that are relevant to in vivo conditions. 8 To candidate NPs for anticancer activity, their side effects against healthy targets such as the protein structure [9][10][11][12] and cell lines [13][14][15] should be assessed. It has been well documented that plasma proteins like albumins are adsorbed on the NP surfaces as the protein corona in the biological system.…”

Section: Introductionmentioning

confidence: 99%

Albumin binding and anticancer effect of magnesium oxide nanoparticles

Behzadi

Sarsharzadeh

Nouri

et al. 2018

IJN

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BackgroundRecently, nanomaterials have moved into biological and medicinal implementations like cancer therapy. Therefore, before clinical trials, their binding to plasma proteins like human serum albumin (HSA) and their cytotoxic effects against normal and cancer cell lines should be addressed.MethodsHerein, the interaction of magnesium oxide nanoparticles (MgO NPs) with HSA was studied by means of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and docking studies. Afterwards, the cytotoxic impacts of MgO NPs on human leukemia cell line (K562) and peripheral blood mononucleated cells (PBMCs) were evaluated by MTT and flow cytometry assays to quantify reactive oxygen species (ROS) generation and apoptosis.ResultsIt was demonstrated that MgO NPs spontaneously form a static complex with HSA molecules through hydrophobic interactions. Docking study based on the size of NPs demonstrated that different linkages can be established between MgO NPs and HSA. The CD investigation explored that MgO NPs did not alter the secondary structure of HSA. Cellular studies revealed that MgO NPs induced cytotoxicity against K562 cell lines, whereas no adverse effects were detected on PBMCs up to optimum applied concentration of MgO NPs. It was exhibited that ROS production mediated by IC50 concentrations of MgO NPs caused apoptosis-associated cell death. The pre-incubation of K562 with ROS scavenger (curcumin) inhibited the impact of MgO NPs -based apoptosis on cell fate, revealing the upstream effect of ROS in our system.ConclusionIn summary, MgO NPs may exhibit strong plasma distribution and mediate apoptosis by ROS induction in the cancer cell lines. These data demonstrate a safe aspect of MgO NPs on the proteins and normal cells and their application as a distinctive therapeutic approach in the cancer treatment.

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Section: Introductionmentioning

confidence: 99%

Albumin binding and anticancer effect of magnesium oxide nanoparticles

Behzadi

Sarsharzadeh

Nouri

et al. 2018

IJN

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“…This leads to cells being physically covered by the nanoparticles and subsequently cell death. The presence of high nanoparticle populations will easily agglomerate at the cell surface and consequently influence the absorption of nutrients and gaseous exchange and thus confer toxicity to cells [ 84 ] Additionally, previous studies have shown that CNP has no any significant cytotoxicity toward several cell lines such as HepG2 human liver cancer cell line, RAW 264.7 mouse macrophage, as well as the A549 cell lines [ 85 , 86 , 87 ].…”

Section: Resultsmentioning

confidence: 99%

Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality

et al. 2020

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The growing incidence of global lung cancer cases against successful treatment modalities has increased the demand for the development of innovative strategies to complement conventional chemotherapy, radiation, and surgery. The substitution of chemotherapeutics by naturally occurring phenolic compounds has been touted as a promising research endeavor, as they sideline the side effects of current chemotherapy drugs. However, the therapeutic efficacy of these compounds is conventionally lower than that of chemotherapeutic agents due to their lower solubility and consequently poor intracellular uptake. Therefore, we report herein a hydrophobically modified chitosan nanoparticle (pCNP) system for the encapsulation of protocatechuic acid (PCA), a naturally occurring but poorly soluble phenolic compound, for increased efficacy and improved intracellular uptake in A549 lung cancer cells. The pCNP system was modified by the inclusion of a palmitoyl group and physico-chemically characterized to assess its particle size, Polydispersity Index (PDI) value, amine group quantification, functional group profiling, and morphological properties. The inclusion of hydrophobic palmitoyl in pCNP-PCA was found to increase the encapsulation of PCA by 54.5% compared to unmodified CNP-PCA samples whilst it only conferred a 23.4% larger particle size. The single-spherical like particles with uniformed dispersity pCNP-PCA exhibited IR bands, suggesting the successful incorporation of PCA within its core, and a hydrophobic layer was elucidated via electron micrographs. The cytotoxic efficacy was then assessed by using an MTT cytotoxicity assay towards A549 human lung cancer cell line and was compared with traditional chitosan nanoparticle system. Fascinatingly, a controlled release delivery and enhanced therapeutic efficacy were observed in pCNP-PCA compared to CNP, which is ascribed to lower IC50 values in the 72-h treatment in the pCNP system. Using the hydrophobic system, efficacy of PCA was significantly increased in 24-, 48-, and 72-h treatments compared to a single administration of the compound, and via the unmodified CNP system. Findings arising from this study exhibit the potential of using such modified nanoparticulate systems in increasing the efficacy of natural phenolic compounds by augmenting their delivery potential for better anti-cancer responses.

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“…CS NPs modified with the amino acids lysine and glutamic acid were designed to chelate copper ions and prevent cytotoxicity and ROS production of CuO NPs. Despite the PC formed, which predominantly contained albumin, the chelating efficiency was not significantly reduced [57] . However, PC may not be created at all.…”

Section: Interaction Between Polysaccharide‐based Nanoparticles and Tmentioning

confidence: 89%

Theranostic Approach for the Protein Corona of Polysaccharide Nanoparticles

Skaličková

Horký

Mlejnková

et al. 2020

The Chemical Record

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Polysaccharide nanoparticles are promising materials in the wide range of disciplines such as medicine, nutrition, food production, agriculture, material science and others. They excel not only in their non-toxicity and biodegradability but also in their easy preparation. As well as inorganic particles, a protein corona (PC) around polysaccharide nanoparticles is formed in biofluids. Moreover, it has been considered that the overall response of the organism to nanoparticles presence depends on the PC. This review summarises scientific publications about the structural chemistry of polysaccharide nanoparticles and their impact on theranostic applications. Three strategies of implementation of the PC in theranostics have been discussed: I) Utilisation of the PC in therapy; II) How the composition of the PC is analysed for specific disease markers; III) How the formed PC can interact with the immune system and enhances the immunomodulation or immunoelimination. Thus, the findings from this review can contribute to improve the design of drug delivery systems. However, it is still necessary to elucidate the mechanisms of nano-bio interactions and discover new connections in nanoscale research.

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Amino acid-modified chitosan nanoparticles for Cu²⁺ chelation to suppress CuO nanoparticle cytotoxicity

Abstract: Chitosan nanoparticles grafted with amino acids suppress the cytotoxicity of engineered nanoparticles by chelating intracellularly released metal ions and scavenging intracellular reactive oxygen species.

Cited by 16 publications

References 65 publications

Albumin binding and anticancer effect of magnesium oxide nanoparticles

Albumin binding and anticancer effect of magnesium oxide nanoparticles

Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality

Theranostic Approach for the Protein Corona of Polysaccharide Nanoparticles

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Amino acid-modified chitosan nanoparticles for Cu2+ chelation to suppress CuO nanoparticle cytotoxicity

Abstract: Chitosan nanoparticles grafted with amino acids suppress the cytotoxicity of engineered nanoparticles by chelating intracellularly released metal ions and scavenging intracellular reactive oxygen species.

Cited by 16 publications

References 65 publications

Albumin binding and anticancer effect of magnesium oxide nanoparticles

Albumin binding and anticancer effect of magnesium oxide nanoparticles

Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality

Theranostic Approach for the Protein Corona of Polysaccharide Nanoparticles

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Amino acid-modified chitosan nanoparticles for Cu²⁺ chelation to suppress CuO nanoparticle cytotoxicity