Amino Acid, Phytochemical Compositions and Antioxidant Activity of Inky Cap Mushroom (Coprinus radiata)

Abstract

Evaluation of the amino acid composition, phytochemical properties and antioxidant activity of Coprinus radiata mushrooms was conducted. Free amino acid composition of fresh mushrooms was analyzed by LC-MS/MS. The phytochemical properties analysis included total phenolic compound content and total flavonoid content of these mushrooms. Furthermore, antioxidant activity assays were performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). Based on the results shown that a high concentration of non-essential amino acids, especially those of arginine and glutamic acid, were observed. However, isoleucine and leucine were major essential amino acids in these mushrooms. The ethanolic extract of C. radiata showed higher phytochemical contents and antioxidant activity than those of the water extract. It suggested that the C. radiata is a natural source containing some essential amino acids, a high amount of phytochemical properties and high antioxidant activity, potentially having medicinal and nutritional properties that provide health benefits.

Exploring the Phytochemical Compositions, Antioxidant Activity, and Nutritional Potentials of Edible and Medicinal Mushrooms

“…The methanolic, ethanolic, and aqueous extracts of P. ostreatus and P. florida possess alkaloids, tannins, and saponins [ 85 ]. The highest TPC concentration was observed in the methanolic extract in both P. ostreatus and P. florida compared to the other solvents, and the concentration was comparable [ 86 ]. On the other hand, steroids are exclusively identified in P. ostreatus within all extracts [ 85 ].…”

“…In a comparison of two extraction methods, ethanol and aqueous extraction, the total phenolic content (TPC) of the inky cap mushroom ( C . radiata ) was analyzed [ 86 ]. The results indicated that the ethanol extract had a higher TPC than the aqueous extract [ 86 ].…”

“…radiata ) was analyzed [ 86 ]. The results indicated that the ethanol extract had a higher TPC than the aqueous extract [ 86 ]. The observed outcomes demonstrated that the polarity of the solvent had an impact on the TPC of the extracts derived from the C. radiata mushroom [ 86 ].…”

“…The results indicated that the ethanol extract had a higher TPC than the aqueous extract [ 86 ]. The observed outcomes demonstrated that the polarity of the solvent had an impact on the TPC of the extracts derived from the C. radiata mushroom [ 86 ]. A comparative analysis was conducted to determine the highest levels of the TPC in commercially and laboratory-prepared extracts of six distinct species of mushrooms [ 95 ].…”