Amino Acid Profiles in Human Tear Fluids Analyzed by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry

Rapid taurine and lactate biomarkers determination with disposable electrochemical detectors

“…Indeed, deviations from normal taurine concentrations in tissues are correlated with several pathological disorders such as Alzheimer's disease, growth retardation, diabetes mellitus [8][9], epilepsy [10], sepsis [11] and several kinds of cancer [12][13][14] as well as different forms of psychosis including trauma [15], depression [16] and schizophrenia [17]. Recently, it has been found that high levels of taurine in tears also has a close relationship with the ocular surface disease and can be used to a biomarker to prevent this pathology [18]. Thus, the rapid and accurate determination of the level of this compound in various tissues and body fluids can be of great interest in the early diagnosis of several important pathologies and diseases.…”

Longitudinal analysis of taurine induced effects on the tear proteome of contact lens wearers and dry eye patients using a RP-RP-Capillary-HPLC–MALDI TOF/TOF MS approach

“…Besides taurine, L-arginine is a well studied amino acid involved in immune response [82,83]. Interestingly, decreased levels of arginine were found to be correlated with a decrease in methionine as a taurine precursor and taurine levels in tears in the course of ocular surface diseases [31]. The inflammatory decrease of arginine levels fits well with an observed upregulation of human cationic transporters 1 and 2 expression under ocular surface disease conditions leading to an increased arginine transport into epithelial cells of the cornea [83].…”

“…The high abundance of taurine in eye tissues [22] e.g. lens [23][24][25], iris ciliar body [22], retina [26][27][28][29] and cornea [24] as well as its presence in tears [23,30,31] correlates with its numerous protective bioactive functions like osmoregulation [25,32], protein stabilization [33] growth factor regulation [34] and cytoprotective activity against a brought range of toxicants [35][36][37][38]. In the last decades there is confidentially growing evidence that taurine protects many ocular tissues from oxidative stress [39,40].…”

Imaging Mass Spectrometric Analysis of Neurotransmitters

“…Weak ionisation and interference by ion signals of matrices during IMS detection can be listed as the technical challenges that have prevented visualisation and quanti cation of amino acid neurotransmitters by IMS. 5) With modi cations to pre-column derivatisation and HPLC/ESI-tandem MS techniques previously reported by Nakatsukasa et al, 50) Toue at al. developed a derivatisation procedure for sections of murine tumourbearing liver tissues and analysed them by MALDI IMS to obtain semi-quantitative spatial information on several amino acids including glutamate, GABA and serine.…”