Amino acid regulation of gene transcription of rat insulin-like growth factor-binding protein-1

Takenaka, Asako; Komori, Kuniharu; Morishita, Teruyuki; Takahashi, Shin‐Ichiro; Hidaka, Takehiko; Noguchi, Tadashi

doi:10.1677/joe.0.164r011

Cited by 30 publications

(12 citation statements)

References 35 publications

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“…FoxO binding sites). In addition, the HNF-3 site of the IGFBP-1 promoter is part of a unit, which confers on this gene a responsiveness to amino acid insufficiency (Takenaka et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Nutritional and hormonal factors control the gene expression of FoxOs, the mammalian homologues of DAF-16

Imae¹,

Fu²,

Yoshida³

et al. 2003

Journal of Molecular Endocrinology

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Transcription factors of the FoxO family in mammals are orthologues of the Caenorhabditis elegans forkhead factor DAF-16, which has been characterized as a target of insulin-like signalling. Three members of this family have been identified in rodents: FoxO1, FoxO3 and FoxO4, originally termed FKHR, FKHRL1 and AFX respectively. A number of in vitro studies have revealed that FoxOs are regulated through phosphorylation in response to insulin and related growth factors, resulting in their nuclear exclusion and inactivation. To clarify the mechanisms involved in the regulation of these factors in vivo, we investigated in the present study whether or not, and if so how, their mRNA levels in rat liver respond to the stimuli of several nutritional and hormonal factors. Imposed fasting for 48 h significantly elevated mRNA levels of FoxO1 (1·5-fold), FoxO3 (1·4-fold), and FoxO4 (1·6-fold). Refeeding for 3 h recovered the induced mRNA levels of FoxO1 and FoxO3 to the control levels, but did not affect that of FoxO4. FoxO1 and FoxO4 mRNA levels were proved to be highly reflective of their protein levels measured by Western immunoblotting. Of the three FoxO genes, FoxO4 only showed altered levels of mRNA (a 1·5-fold increase) in response to a protein-free diet. Streptozotocin-induced diabetes for 28 days decreased hepatic mRNA levels of FoxO1 and FoxO3 and increased the level of FoxO4 mRNA, but short-term (7 days) diabetes had fewer effects on the expression of these genes. Insulin replacement partially restored the FoxO1 and FoxO4 mRNA levels, but had no effect on the FoxO3 mRNA level. Daily administration for 1 week of dexamethasone, a synthetic glucocorticoid, increased the mRNA levels of FoxO1 (1·8-fold) and FoxO3 (2·4-fold). These results show that the FoxO genes respond differently to nutritional and hormonal factors, suggesting a new mechanism for the regulation of FoxO-dependent gene expression by these factors. Moreover, changes of FoxO1 and FoxO4 in the nucleus in response to fasting also suggest that the regulation of nucleus/cytoplasm translocation actually functions in vivo.

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“…FoxO binding sites). In addition, the HNF-3 site of the IGFBP-1 promoter is part of a unit, which confers on this gene a responsiveness to amino acid insufficiency (Takenaka et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Nutritional and hormonal factors control the gene expression of FoxOs, the mammalian homologues of DAF-16

Imae¹,

Fu²,

Yoshida³

et al. 2003

Journal of Molecular Endocrinology

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“…The authors have identi-ˆe d several genes that are down-regulated 34) or upregulated 41,42) by protein malnutrition and have been trying to unveil the mechanism for this regulation by protein nutrition. We therefore attempted to investigate if the pathway involved in the regulation of these genes also regulated the genes for collagens and its related proteins.…”

Section: Discussionmentioning

confidence: 99%

Effects of Protein Deprivation on α1(I) and α1(III) Collagen and Its Degrading System in Rat Skin

Oishi

Ohnuki

et al. 2002

Bioscience, Biotechnology, and Biochemistry

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“…At present, the effects of nutrients on expression of some growth‐regulatory genes, such as the growth hormone insulin‐like growth factor (GH/IGF) axis, have been postulated and demonstrated. The deficiency of essential amino acid may affect the secretion of IGF‐I in rats or pigs (Takenaka et al., 2000; Katsumata et al., 2002), and a hypothesis was offered that amino acid (such as lysine and methionine) may affect the synthesis and secretion of the GH/IGF axis and then regulate the growth of growing rabbits.…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary methionine on growth performance and insulin-like growth factor-I mRNA expression of growing meat rabbits

Zhang

2010

Journal of Animal Physiology and Animal Nutrition

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An experiment was conducted to determine the effects of different amounts of dietary methionine on growth performance, serum protein, growth hormone (GH), insulin-like growth factor-I (IGF-I) concentrations and IGF-I mRNA expression of growing meat rabbits. One hundred weaned growing meat rabbits were allocated to individual cages and randomly divided into five groups. The methionine addition concentrations of the five groups were 0, 2, 4, 6 and 8 g/kg diet (as-fed basis) and sulphur amino acids (SAA) concentrations ranging from 3.8 to 11.6 g/kg diet, respectively. The results obtained were as follows: the average daily gain of 2, 4 and 6 g/kg diet groups was higher than that of 0 g/kg diet group (p < 0.01). The feed gain ratio of the 4 g/kg diet group was lower than those of 0 and 8 g/kg diet group (p < 0.01). Methionine concentrations did not affect serum urea nitrogen, total protein, insulin and IGF-I concentration (p > 0.05). The quadratic effects of methionine on the serum concentration of albumin (Alb) and GH were obtained (p = 0.013, p = 0.018). The quadratic effect of methionine amount on IGF-I mRNA expression was obtained (p = 0.045). The serum concentration of Alb of the 4 g/kg diet group was higher than those of 0 and 8 g/kg diet group (p < 0.01). The serum concentration of GH of 8 g/kg diet group was higher than that of the 0 g/kg diet group (p < 0.05). The liver IGF-I mRNA expression of 4 g/kg diet group was higher than those of the 0 and 8 g/kg diet group (p < 0.05). Providing a diet mainly consisted of corn, wheat bran and peanut vine, the optimum dietary methionine addition concentration and SAA concentration for a weaner to 2-month-old growing meat rabbits were shown to be 2 and 5.7 g/kg diet respectively.

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Amino acid regulation of gene transcription of rat insulin-like growth factor-binding protein-1

Cited by 30 publications

References 35 publications

Nutritional and hormonal factors control the gene expression of FoxOs, the mammalian homologues of DAF-16

Nutritional and hormonal factors control the gene expression of FoxOs, the mammalian homologues of DAF-16

Effects of Protein Deprivation on α1(I) and α1(III) Collagen and Its Degrading System in Rat Skin

Effect of dietary methionine on growth performance and insulin-like growth factor-I mRNA expression of growing meat rabbits

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