Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design

Pletneva, N.V.; Maksimov, Eugene G.; Protasova, Elena A.; Mamontova, Anastasia V.; Simonyan, Tatiana R.; Ziganshin, Rustam H.; Lukyanov, Konstantin A.; Muslinkina, Liya; Pletnev, Sergei; Bogdanov, Alexey M.; Плетнев, В. З.

doi:10.1016/j.csbj.2021.05.017

Computational and Structural Biotechnology Journal

2021

DOI: 10.1016/j.csbj.2021.05.017

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Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design

N.V. Pletneva

Eugene G. Maksimov

Elena A. Protasova

et al.

Abstract: Graphical abstract

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2024

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Decoding mEos4b Day-Long Maturation and Engineering Fast Maturing Variants

Maity,

Glushonkov,

Ayala

et al. 2024

Preprint

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The maturation speed of fluorescent proteins is a crucial parameter that influences cellular brightness, effective labeling efficiency and temporal resolution in fluorescence microscopy. Green-to-red photoconvertible fluorescent proteins (PCFPs) used in pulse-chase experiments and super-resolution techniques such as Photoactivated Localization Microscopy (PALM), single-particle-tracking PALM (sptPALM) and Minimal Fluorescence Photon Fluxes Microscopy (MINFLUX) may be hampered by slow maturation. We systematically characterized the maturation speed of mEos-derived PCFPs in E. coli and found that, in contrast to pcStar and mEosEM, several variants such as mEos2, mEos3.1, mEos3.2 and mEos4b mature extremely slowly. Strikingly, the oxidation step in those PCFPs is fast and not rate-limiting. Through a rational mutagenesis approach, we developed a strategy to reduce the day-long maturation time of mEos4b by nearly two orders of magnitude without significantly impacting its molecular brightness and photophysical performance under single-molecule imaging conditions.

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Decoding mEos4b Day-Long Maturation and Engineering Fast Maturing Variants

Maity,

Glushonkov,

Ayala

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design

Abstract: Graphical abstract

Cited by 1 publication

References 55 publications

Decoding mEos4b Day-Long Maturation and Engineering Fast Maturing Variants

Decoding mEos4b Day-Long Maturation and Engineering Fast Maturing Variants

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