Amino acid secretion influences the size and composition of copper carbonate nanoparticles synthesized by ureolytic fungi

Liu, Feixue; Csetényi, László; Gadd, Geoffrey Michael

doi:10.1007/s00253-019-09961-2

Cited by 47 publications

(34 citation statements)

References 56 publications

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“…In other studies, significant amounts of extracellular protein in fungal supernatants were removed by precipitation of copper carbonate (Li and Gadd 2017a, b;Liu et al 2019). Here, extracellular protein was only partly removed during oxalate precipitation, while the extracellular polysaccharide concentration did not show much change compared to the control.…”

Section: Discussionmentioning

confidence: 57%

“…Calcium recovery of more than 90% was achieved from a calcium-rich industrial wastewater using bacterial MICP (Hammes et al 2003). Supernatants obtained from ureolytic fungi are also very efficient in forming copper and other metal carbonate nanoparticles for biorecovery (Li et al , 2015(Li et al , 2019Li and Gadd, 2017a, b;Liu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Yang

Song

Ferrier

et al. 2019

Appl Microbiol Biotechnol

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In this research, the capabilities of culture supernatants generated by the oxalate-producing fungus Aspergillus niger for the bioprecipitation and biorecovery of cobalt and nickel were investigated, as was the influence of extracellular polymeric substances (EPS) on these processes. The removal of cobalt from solution was >90% for all tested Co concentrations: maximal nickel recovery was >80%. Energy-dispersive X-ray analysis (EDXA) and X-ray diffraction (XRD) confirmed the formation of cobalt and nickel oxalate. In a mixture of cobalt and nickel, cobalt oxalate appeared to predominate precipitation and was dependent on the mixture ratios of the two metals. The presence of EPS together with oxalate in solution decreased the recovery of nickel but did not influence the recovery of cobalt. Concentrations of extracellular protein showed a significant decrease after precipitation while no significant difference was found for extracellular polysaccharide concentrations before and after oxalate precipitation. These results showed that extracellular protein rather than extracellular polysaccharide played a more important role in influencing the biorecovery of metal oxalates from solution. Excitation-emission matrix (EEM) fluorescence spectroscopy showed that aromatic protein-like and hydrophobic acid-like substances from the EPS complexed with cobalt but did not for nickel. The humic acid-like substances from the EPS showed a higher affinity for cobalt than for nickel.

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Section: Discussionmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Yang

Song

Ferrier

et al. 2019

Appl Microbiol Biotechnol

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“…After the organic substances were completely washed off, struvite crystals collected in the experimental group at Mg/Ca ratio of 12 were also analyzed using XPS (Thermo ESCALAB 250XI, ThermoFisher, Waltham, MA, USA) with a step size of 0.05 eV. Charge correction was performed with O1s (530.90 Ev) as standard binding energy [49].…”

Section: Characteristics Of Minerals Induced By C Freundii Zw123mentioning

confidence: 99%

Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Sun

Zhao

et al. 2020

Minerals

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In this study, a facultative anaerobic strain isolated from marine sediments and identified as Citrobacter freundii, was used to induce the precipitation of carbonate and phosphate minerals in the laboratory under anaerobic conditions. This is the first time that the ability of C. freundii ZW123 to precipitate carbonate and phosphate minerals has been demonstrated. During the experiments, carbonic anhydrase, alkaline phosphatase and ammonium released by the bacteria not only promoted an increase in pH, but also drove the supersaturation and precipitation of carbonate and phosphate minerals. The predominant bio-mediated minerals precipitated at various Mg/Ca molar ratios were calcite, vaterite, Mg-rich calcite, monohydrocalcite and struvite. A preferred orientation towards struvite was observed. Scanning transmission electron microscopy (STEM) and elemental mapping showed the distribution of magnesium and calcium elements within Mg-rich calcite. Many organic functional groups, including C=O, C–O–C and C–O, were detected within the biominerals, and these functional groups were also identified in the associated extracellular polymeric substances (EPS). Fifteen kinds of amino acid were detected in the biotic minerals, almost identical to those of the EPS, indicating a close relationship between EPS and biominerals. Most amino acids are negatively charged and able to adsorb cations, providing an oversaturated microenvironment to facilitate mineral nucleation. The X-ray photoelectron spectroscopy (XPS) spectrum of struvite shows the presence of organic functional groups on the mineral surface, suggesting a role of the microorganism in struvite precipitation. The ZW123 bacteria provided carbon and nitrogen for the formation of the biotic minerals through their metabolism, which further emphasizes the close relationship between biominerals and the microorganisms. Thermal studies showed the enhanced thermal stability of biotic minerals, perhaps due to the participation of the bacteria ZW123. The presence of amino acids such as Asp and Glu may explain the high magnesium content of some calcites. Molecular dynamics simulations demonstrated that the morphological change and preferred orientation were likely caused by selective adsorption of EPS onto the various struvite crystal surfaces. Thus, this study shows the significant role played by C. freundii ZW123 in the bioprecipitation of carbonate and phosphate minerals and provides some insights into the processes involved.

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“…Avantage2.16 software (2.16, Thermo Fisher, Shanghai, China) was used to analyze the XPS spectra core-level lines for curve fitting. All spectra were referenced to the O 1s peak of carbonate at 530.9 eV [41].…”

Section: Characterization Of Precipitatesmentioning

confidence: 99%

“…The C 1s peak (Figure 9c) at 280.86 eV indicates that carbon is bound only to carbon and hydrogen (C-(C, H)), and the peak at 284.86 eV illustrates that carbon is singly-bound to oxygen or nitrogen (C-(O, N)) from ethers, alcohols, amines, and/or amides [77]. Nitrogen appears at a binding energy of 395.56 eV (Figure 9d), due to the presence of amine or amide groups of proteins [41]. The oxygen peak (O 1s ) at 527.11 eV (Figure 9e) indicates that oxygen is double bonded with carbon or phosphorus (C=O or P=O) from carboxylic acids, carboxylates, esters, carbonyls, amides, or phosphoryl groups [78].…”

Section: Characterization Of Surface Chemistry With Xpsmentioning

confidence: 99%

Biomineralization of Monohydrocalcite Induced by the Halophile Halomonas Smyrnensis WMS-3

et al. 2019

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The halophilic bacterium Halomonas smyrnensis from a modern salt lake used in experiments to induce biomineralization has resulted in the precipitation of monohydrocalcite and other carbonate minerals. In this study, a Halomonas smyrnensis WMS‐3 (GenBank:MH425323) strain was identified based on 16S rDNA homology comparison, and then cultured in mediums with 3% NaCl concentration to induce monohydrocalcite at different Mg/Ca molar ratios of 0, 2, 5, 7, and 9. The growth curve of WMS‐3 bacteria, pH values, NH4+ concentration, HCO3− and CO32− concentration, carbonic anhydrase (CA) activity, and the changes in Ca2+ and Mg2+ ion concentration were determined to further explore the extracellular biomineralization mechanism. Moreover, the nucleation mechanism of monohydrocalcite on extracellular polymeric substances (EPS) was analyzed through studying ultrathin slices of the WMS‐3 strain by High resolution transmission electron microscopy (HRTEM), Selected area election diffraction (SAED), Scanning transmission electron microscopy (STEM), and elemental mapping, besides this, amino acids in the EPS were also analyzed. The results show that pH increased to about 9.0 under the influence of ammonia and CA activity. The precipitation ratio (%, the ratio of the mass/volume concentration) of the Ca2+ ion was 64.32%, 62.20%, 60.22%, 59.57%, and 54.42% at Mg/Ca molar ratios of 0, 2, 5, 7, and 9, respectively, on the 21st day of the experiments, and 6.69%, 7.10%, 7.74%, 8.09% for the Mg2+ ion concentration at Mg/Ca molar ratios 2, 5, 7, and 9, respectively. The obtained minerals were calcite, Mg‐rich calcite, aragonite, and hydromagnesite, in addition to the monohydrocalcite, as identified by X-ray diffraction (XRD) analyses. Monohydrocalcite had higher crystallinity when the Mg/Ca ratio increased from 7 to 9; thus, the stability of monohydrocalcite increased, also proven by the thermogravimetry (TG), derivative thermogravimetry (DTG) and differential scanning calorimetry (DSC) analyses. The C=O and C–O–C organic functional groups present in/on the minerals analyzed by Fourier transform infrared spectroscopy (FTIR), the various morphologies and the existence of P and S determined by scanning electron microscope-energy dispersive spectrometer (SEM‐EDS), the relatively more negative stable carbon isotope values (−16.91‰ to −17.91‰) analyzed by a carbon isotope laser spectrometer, plus the typical surface chemistry by XPS, all support the biogenesis of these mineral precipitates. Moreover, Ca2+ ions were able to enter the bacterial cell to induce intracellular biomineralization. This study is useful to understand the mechanism of biomineralization further and may provide theoretical reference concerning the formation of monohydrocalcite in nature.

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Amino acid secretion influences the size and composition of copper carbonate nanoparticles synthesized by ureolytic fungi

Cited by 47 publications

References 56 publications

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Biorecovery of cobalt and nickel using biomass-free culture supernatants from Aspergillus niger

Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Biomineralization of Monohydrocalcite Induced by the Halophile Halomonas Smyrnensis WMS-3

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