1988
DOI: 10.1021/bi00420a030
|View full text |Cite
|
Sign up to set email alerts
|

Amino acid sequence and posttranslational modifications of human factor VIIa from plasma and transfected baby hamster kidney cells

Abstract: Blood coagulation factor VII is a vitamin K dependent glycoprotein which in its activated form, factor VIIa, participates in the coagulation process by activating factor X and/or factor IX in the presence of Ca2+ and tissue factor. Three types of potential posttranslational modifications exist in the human factor VIIa molecule, namely, 10 gamma-carboxylated, N-terminally located glutamic acid residues, 1 beta-hydroxylated aspartic acid residue, and 2 N-glycosylated asparagine residues. In the present study, th… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

7
186
0
5

Year Published

1992
1992
2004
2004

Publication Types

Select...
4
3

Relationship

1
6

Authors

Journals

citations
Cited by 290 publications
(198 citation statements)
references
References 33 publications
7
186
0
5
Order By: Relevance
“…Recombinant fVIla was purified according to Thim et al [12]. The y35 and E35 isoforms of fVIIa were separated by ion-exchange chromatography on a NucleoPac PA-100 column using a ff4).85 M linear gradient of ammonium acetate in 10 mM Tris, 10 mM Bis-Tris propane, pH 9.2.…”
Section: Proteins Materials and Standard Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Recombinant fVIla was purified according to Thim et al [12]. The y35 and E35 isoforms of fVIIa were separated by ion-exchange chromatography on a NucleoPac PA-100 column using a ff4).85 M linear gradient of ammonium acetate in 10 mM Tris, 10 mM Bis-Tris propane, pH 9.2.…”
Section: Proteins Materials and Standard Methodsmentioning
confidence: 99%
“…Therefore, it was of interest to us to investigate the consequences of mutating this residue in fVII. In addition, the production of factors VII and IX in mammalian cell cultures is fraught by the problem of partial y-carboxylation of the most C-terminal Gla residues [12,13] and we also wanted to determine the effects of this specific modification of Glu-35 in fVlI.…”
Section: Introductionmentioning
confidence: 99%
“…Recombinant FVIIa [7], 1 mg/ml was dialyzed against !0 mM Tris. HCI, pH 8,6 containing 75 mM NaCl and 5 mM CaCl:.…”
Section: Methodsmentioning
confidence: 99%
“…Reverse-phase high performance liquid chromatography (RP-HPLC), amino acid analysis and amino acid sequence analysis were performed as previously described [7,10]. ).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation