Amino-Functionalization of Carbon Nanotubes by Using a Factorial Design: Human Cardiac Troponin T Immunosensing Application

Freitas, Tatianny A.; Mattos, Alessandra B.; Silva, Bárbara V. M.; Dutra, Rosa F.

doi:10.1155/2014/929786

Cited by 26 publications

(10 citation statements)

References 37 publications

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“…The weak peak at 1070 cm −1 in Figure S1 C is from the C−N stretching vibration of the primary amine end groups [ 22 ]. Broadening of the peak at 3440 cm −1 further confirms the presence of –NH stretching frequency [ 23 ]. These observations confirm the formation of amino functional group on the surface of MWCNTs.…”

Section: Methodsmentioning

confidence: 87%

Construction of an Electrochemical Sensor Based on Carbon Nanotubes/Gold Nanoparticles for Trace Determination of Amoxicillin in Bovine Milk

Muhammad

Yusof

Hajian

et al. 2016

Sensors

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In this work, a novel electrochemical sensor was fabricated for determination of amoxicillin in bovine milk samples by decoration of carboxylated multi-walled carbon nanotubes (MWCNTs) with gold nanoparticles (AuNPs) using ethylenediamine (en) as a cross linker (AuNPs/en-MWCNTs). The constructed nanocomposite was homogenized in dimethylformamide and drop casted on screen printed electrode. Field emission scanning electron microscopy (FESEM), energy dispersive X-Ray (EDX), X-Ray diffraction (XRD) and cyclic voltammetry were used to characterize the synthesized nanocomposites. The results show that the synthesized nanocomposites induced a remarkable synergetic effect for the oxidation of amoxicillin. Effect of some parameters, including pH, buffer, scan rate, accumulation potential, accumulation time and amount of casted nanocomposites, on the sensitivity of fabricated sensor were optimized. Under the optimum conditions, there was two linear calibration ranges from 0.2–10 µM and 10–30 µM with equations of Ipa (µA) = 2.88C (µM) + 1.2017; r = 0.9939 and Ipa (µA) = 0.88C (µM) + 22.97; r = 0.9973, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) were calculated as 0.015 µM and 0.149 µM, respectively. The fabricated electrochemical sensor was successfully applied for determination of Amoxicillin in bovine milk samples and all results compared with high performance liquid chromatography (HPLC) standard method.

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Section: Methodsmentioning

confidence: 87%

Construction of an Electrochemical Sensor Based on Carbon Nanotubes/Gold Nanoparticles for Trace Determination of Amoxicillin in Bovine Milk

Muhammad

Yusof

Hajian

et al. 2016

Sensors

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“…The calculated limit of detection (LOD = 3 x standard deviation of the intercept / slope) was 0.016 ng mL À1 . This immunosensor showed high sensitivity and can be used for TnT determination in routine clinical analysis, since the cutoff was approximately 0.02 ng mL À 1 in the AMI diagnosis [32]. As can be seen in Table 1, the linear range, LOD and incubation time for the proposed immunosensor for TnT is comparable with or even better than those of other recently published methods involving the use of biosensors.…”

Section: Optimization Of the Proposed Methodsmentioning

confidence: 60%

Label‐free Electrochemical Immunosensor for Cardiac Troponin T Based on Exfoliated Graphite Nanoplatelets Decorated with Gold Nanoparticles

et al. 2017

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This paper describes the application of exfoliated graphite nanoplatelets (xGnP) decorated with gold nanoparticles (AuNP) for the development of a label‐free electrochemical immunosensor for the determination of human cardiac troponin T (TnT), an important cardiac biomarker in the diagnosis of acute myocardial infarction (AMI). Heparin‐stabilized AuNP (AuNP‐Hep) were synthesized, characterized and supported on xGnP. The material obtained (AuNP‐Hep‐xGnP) was used as a platform to immobilize the anti‐TnT by adsorption and this was then applied in the construction of an immunosensor. Under optimized conditions, using differential pulse voltammetry (DPV) and an incubation time of 20 min, the proposed immunosensor showed linearity in the range of 0.050 to 0.35 ng mL−1 TnT, with a calculated limit of detection of 0.016 ng mL−1. The interday precision (n=7) showed a coefficient of variation of 6.5 %. Some potential interferents commonly present in blood plasma samples were investigated and the degree of interference was found to be low (less than 10 %), demonstrating adequate selectivity for analytical applications. The biosensor was successfully applied in the determination of TnT in fortified samples of human blood plasma.

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“…It showed a broad linear range (0.02 to 0.32 ng/mL) and a low limit of detection, 0.016 ng/mL. [83] Another sandwich-type immunosensor for the detection of cTnT based on carbon nanotubes supported by a conductive polyethyleneimine film has achieved a low limit of the detection of 0.033 ng/mL and a linear range between 0.1 and 10 ng/mL. [24] Amperometric response is generated by peroxidase reaction with substrate in chronoamperometry detection.…”

Section: Carbon Nanotubesmentioning

confidence: 96%

Nanomaterials for Advancing the Health Immunosensor

Rodríguez¹,

Trindade²,

Cabral³

et al. 2015

Biosensors - Micro and Nanoscale Applications

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Nanotechnoloμy has exerted a siμniλicant impact in the development oλ biosensors allowinμ more sensible analytical methods. In health applications, the main challenμe oλ the immunoassay is to reach the suitable limit oλ detection, recoμnizinμ diλλerent analytes in complex samples like whole blood, serum, urine, and other bioloμical λluids. Diλλerent nanomaterials, includinμ metallic, silica and maμnetic nanoparticles, quantum dots, carbon nanotubes, and μraphene, have been applied, mainly to improve charμe electron transλer, catalytic activity, amount oλ immobilized biomolecules, low-backμround current, siμnal-to-noise ratio that consequently increase the sensitivity oλ immunosensors. Given the μreat impact oλ nanotechnoloμy, this chapter intends to discuss new aspects oλ nanomaterials relatinμ to immunosensor advancement. Keywords:Immunosensors, immunoassay, nanosensor, nanomaterial . IntroductionA major challenμe λaced by health proμrams is the maintenance and availability oλ diaμnostic tests that are required not only in inpatient or outpatient hospital but also λor an improved epidemioloμical survey. In many cases, the absence oλ laboratory testinμ or delay oλ diaμnosis μenerates neμative economic impacts, resultinμ in unnecessary hospitalization, intercurrence, and in some cases implications on the μlobal liλe quality oλ patients and underreportinμ oλ surveillance. In this context, the development oλ practical, λast, and reliable analytical methods is imperative.Biosensors have been considered one oλ the more attractive analytical methods. They are biodevices capable oλ transλorminμ an interaction with speciλic analytes into an electrical siμnal by a transducer, includinμ a biorecoμnition element. [ ] Pharmaceutical industries and users oλ rapid tests λrom the United States and Europe are unanimous in statinμ that biosensors, mainly those based on point-oλ-care testinμ POCT , or bedside testinμs are a practical technoloμy, reμarded as a short-, medium-, and lonμ-term trend. Amonμ several advantaμes, POCT can provide immediate responses results in λew minutes or in real time , samples do not need to be transported to the analytical phase in situ monitorinμ and require μenerally small volumes oλ samples, and the users can be skilled or unskilled and present better costeλλective analyses compared with conventional technoloμies used in clinical diaμnostic userλriendly technoloμy . One oλ the most widely useλul POCT is the μlucometer, which measures μlucose levels with accuracy by requirinμ a sinμle drop oλ blood. The rapid μlucose measurement is very important in trials to avoid serious adverse eλλects stemminμ λrom diabetes, includinμ seizures, coma, or even death. Worldwide, some diabetic outpatients have been beneλited by POCTs.Althouμh there is a μreat promisinμ market dedicated to health λor the detection oλ diseases and therapeutic monitorinμ, biosensors are not yet entirely broadcast, especially those devoted to nonenzymatic reactions, i.e., biosensors based on the aλλinity between antiμen antibo...

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Amino-Functionalization of Carbon Nanotubes by Using a Factorial Design: Human Cardiac Troponin T Immunosensing Application

Cited by 26 publications

References 37 publications

Construction of an Electrochemical Sensor Based on Carbon Nanotubes/Gold Nanoparticles for Trace Determination of Amoxicillin in Bovine Milk

Construction of an Electrochemical Sensor Based on Carbon Nanotubes/Gold Nanoparticles for Trace Determination of Amoxicillin in Bovine Milk

Label‐free Electrochemical Immunosensor for Cardiac Troponin T Based on Exfoliated Graphite Nanoplatelets Decorated with Gold Nanoparticles

Nanomaterials for Advancing the Health Immunosensor

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