Aminopeptidase activities inPeptostreptococcusspp. are statistically correlated to gelatin hydrolysis

Abstract

One hundred Peptostreptococcus isolates from five species were assessed for their ability to hydrolyze gelatin. Most Peptostreptococcus magnus (95.8%) and Peptostreptococcus micros isolates (79.0%) hydrolyzed gelatin in contrast to Peptostreptococcus asaccharolyticus (8.0%), Peptostreptococcus anaerobius (10.0%), and Peptostreptococcus prevotii isolates (16.7%). Gelatin hydrolysis in Peptostreptococcus magnus and Peptostreptococcus micros isolates correlated (r = 0.80; P = 0.0019) with more aminopeptidases produced than Peptostreptococcus asaccharolyticus, Peptostreptococcus anaerobius, or Peptostreptococcus prevotii. The five species were further classified into three groups using the extended Tukey test (P < 0.0001) based on the mean percentage of aminopeptidases produced by each species with Peptostreptococcus magnus and Peptostreptococcus micros belonging to group I, Peptostreptococcus asaccharolyticus and Peptostreptococcus prevotii belonging to group II, and Peptostreptococcus anaerobius forming group III. An analysis of possible proteolytic activity of four selected Peptostreptococcus magnus isolates indicated that only 5 of 11 substrates were hydrolyzed as compared to a control isolate of Porphyromonas gingivalis W83, which had a strong proteolytic profile. Therefore, gelatin hydrolysis by Peptostreptococcus spp., in particular Peptostreptococcus magnus and Peptostreptococcus micros, is probably due to a variety of aminopeptidases rather than proteinases.

Molecular Analysis of Microbial Diversity in Advanced Caries

“…and the Propionibacterium sp. oral strain FMA5, which accounted for 30% of the bacterial population in sample 10, have been shown to degrade proteins such as gelatin and albumin (10,24,34). Such bacteria may therefore provide peptides and amino acids required by other bacteria within the polymicrobial population.…”

Bacterial Artificial Chromosome Library of Finegoldia magna ATCC 29328 for Genetic Mapping and Comparative Genomics

“…Finegoldia magna ATCC 29328 strain was purchased from the American Type Culture Collection and was grown anaerobically at 37 C in liquid or on agar plates using Gifu Anaerobic Medium (GAM) (Nissui) for [13][14][15][16][17][18][19][20][21][22][23][24] TM Electrocompetent E. coli (Epicentre), carrying recombinant pBeloBAC11, was cultured in LuriaBertani (LB) medium or on LB agar plates at 37 C for 24 hr. Where required, 12.5 µg/ml chloramphenicol (CM), 25 µg/ml isopropyl β-D-thiogalactopyranoside (IPTG) and 50 µg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal) were added.…”

“…The following proteins have recently been the focus of research interest as potential virulence factors for F. magna-induced infectious diseases: a human serum albumin (HSA)-binding protein termed protein PAB (5, 6), an immunoglobulin light chain-binding protein, protein L (14,15), and proteolytic enzymes (17,22). The partial sequence of an urPAB protein homolog, which is one of these virulence factors (5, 6) of F. magna, was found by our BAC-end sequencing (See BAC-end locus no.107 in Table 1).…”

Necrotizing Fasciitis: A Complication of Percutaneous Coronary Revascularization

“…These anaerobes have the potential to modify local immune function, coagulation, and other physiologic conditions via different elements present on the bacterial capsule and enzyme production encoded by bacterial DNA (Table 2). [24][25][26][27][33][34][35][36][37][38][39][40] During PTCA, moderate sedation, arterial puncture, and local manipulation of catheters and guide wires predispose to poor oxygenation of subcutaneous tissues in the groin. How then does this progress to NF?…”

“…Under these conditions, the interaction between microbes such as Peptostreptococcus and subcutaneous structures such as activated vascular endothelial cells, adipocytes, and resident immune system cells results in cytokine production, inflammatory cell migration, vascular thrombosis, and tissue ischemia leading to nerve and fat necrosis. [33][34][35][36][37][38][39][40] Table 2 summarizes the mechanisms by which Peptostreptococcus species destroy subcutaneous tissue by inflammation and necrosis. The clinical features associated with this process are hematoma development, intense pain, skin discoloration, subcutaneous gas production, a foul-smelling discharge (dishwater pus) and intense edema requiring emergency debridement, and in some cases, fasciotomy.…”