Aminosilane as an effective binder for hydroxyapatite-gelatin nanocomposites

Abstract

Aminosilane has been explored as an alternative chemical linker to facilitate the binding and solidification of hydroxyapatite-gelatin nanocomposite at room temperature, which was synthesized using co-precipitation method in the presence of gelatin. This aminosilane treatment was found effective at low concentration (~25 μL/mL) and the solidification and dehydration of hydroxyapatite-gelatin slurry completes within hours depending on the amount of aminosilane. The resulting sample exhibits compressive strength of 133 MPa, about 40% higher than glutaraldehyde treated samples, and shows good biocompatibility based on cell adhesion, proliferation, alkaline phosphate synthesis, and mineralization studies.

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

“…However, the nonporous base used (for the scaffold retention) may take more time to degrade, particularly on HAP-GEL scaffolds (without TiO 2 ), since these inherently possess greater amount of the aminosilane binder or crosslinker. 6 In addition, higher concentrations of the aminosilane may reduce the porosity and limit the migration of osteoprogenitor cells residing on the dorsal and ventral periosteum in this calvaria model. 6,51 The formation of a microvascular network in porous scaffolds was not the main goal of this study, and therefore, it was not thoroughly evaluated.…”

“…6 In addition, higher concentrations of the aminosilane may reduce the porosity and limit the migration of osteoprogenitor cells residing on the dorsal and ventral periosteum in this calvaria model. 6,51 The formation of a microvascular network in porous scaffolds was not the main goal of this study, and therefore, it was not thoroughly evaluated. However, in the future, these HAP-GEL constructs may require loading of endothelial progenitor cells to generate microvascularization and promote a faster regeneration.…”

“…6,7,21 Briefly, the HAP coprecipitation reaction was induced at 38°C in soluble GEL titrated with the Ca(OH) 2 solution. After the coprecipitation, the slurry was centrifuged and the supernatant removed.…”

“…5 HAP-GEL cross-linked with an aminosilane was previously found to exhibit an appropriate compressive strength (100 MPa), biocompatibility and facilitate surface bone ingrowth. 6,7 The loading of cells on alloplastic grafts such as porous calciumbased scaffolds in the hydroxyapatite form (HAP), followed by in vivo implantation in large bone defects, had been previously shown to promote healing. 8,9 Moreover, injectable and formable calcium-based composites with natural polymeric phases (e.g., gelatin) can further increase their clinical applicability.…”

Fundamental Properties of Bioceramics and Biocomposites

“…Biological tests showed good adhesion and proliferation of human MSCs. Luo et al prepared a HA-gelatin nanocomposite using a coprecipitation method in the presence of aminosilane as a chemical linker to facilitate the binding and solidification of the HA-gelatin nanocomposite [70]. This nanocomposite exhibited a compressive strength of 133 MPa and showed a good biocompatibility based on cell adhesion, proliferation, alkaline phosphate synthesis, and mineralization studies.…”