Ammonia Accumulation of Novel Nitrogen-Fixing Bacteria

Iwata, Ken-ichi; Yu, San San; Azlan, Nik Noor Azlin binti; Omori, Toshio

doi:10.5772/30654

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…As a consequence, it caused color changing of the media. The finding was compatible with Gothwal et al , Iwata et al who described that nitrogen activity of Azotobacter beijerinckii and Lysobacter enzymogenes DMS 2043T and rhizospheric bacterial isolates were screened by changing the color of BTB containing nitrogen free media, and these strains showed a color change in BTB containing media, suggesting excretion of ammonia [15, 16]. …”

Section: Resultssupporting

confidence: 85%

Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues

Latt¹,

Yu²,

Kyaw³

et al. 2018

TOMICROJ

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Introduction: Azomonas agilis, a nitrogen-fixing bacterium, was isolated from rhizospheric soil in central Myanmar.Methods & Materials: The nitrogen-fixing activity of this bacterium was detected by plate screening method using glucose nitrogen free mineral medium and ammonium test-kit Cellulolytic activity was screened by plat assay and detected by Dinitrosalicyclic acid method (DNS).Results & Discussion: The isolated A. agilis grew in media containing 3-12% of NaCl, although the growth became poor when NaCl concentrations increased. Among various carbon sources, sucrose was the best source for ammonium accumulation of this bacterium, whereas arabinose was not the suitable carbon source. Although the nitrogen-fixing activity of A. agilis was highest after one week incubation, cellulase enzyme production was highest after 2-3 days of incubation. It was observed that cellulase enzyme activity of A. agilis for cellulose and sodium carboxymethyl cellulose (CMC) was almost the same. Three agricultural wastes were used to detect the cellulase enzyme activity of A. agilis, cellulase activity was better on filter paper as a substrate when compared to rice-straw and sawdust.Conclusion:So, the isolated A. agilis has high potential as an effective bacterial strain to use in sustainable agriculture and degradation of some agricultural residues.

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Section: Resultssupporting

confidence: 85%

Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues

Latt¹,

Yu²,

Kyaw³

et al. 2018

TOMICROJ

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“…Semakin tinggi konsentrasi nitrogenase maka aktivitas nitrogenase pun akan semakin tinggi (Hardy et al, 1996). Selain itu, sumber karbon yang berbeda dapat mempengaruhi kemampuan sel bakteri dalam mengeksresikan amonium (Iwata et al, 2012).…”

Section: Kemampuan Bakteri Menambat Nitrogenunclassified

Characteristic and viability of nitrogen fixation bacteria and phosphate solubilizing bacteria in carrier media

Umadi

2023

J. Il. Tan. Lingk.

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Konsorsium bakteri penambat nitrogen dan pelarut fosfat dapat dijadikan pupuk hayati untuk meningkatkan ketersediaan hara tanaman seperti nitrogen (N) dan fosfor (P). Penelitian ini bertujuan untuk mempelajari karakter dan kompatibilitas bakteri penambat nitrogen dan bakteri pelarut fosfat serta membandingkan viabilitasnya dalam media pembawa yang berbeda, yaitu pada biochar berbahan arang sekam dan tongkol jagung. Penelitian ini terdiri dari uji patogenitas (uji hipersensitivitas dan uji aktivitas hemolitik), uji kemampuan penambatan nitrogen (uji Nessler), uji kemampuan pelarut fosfat pada media Pikovskaya padat, uji kompatibilitas, dan uji viabilitas. Hasil penelitian menunjukkan isolat KPB4 merupakan patogen terhadap hewan atau manusia (uji aktivitas hemolitik). Isolat bakteri penambat nitrogen (KBP1, KBP2, dan KBP5) memiliki kelarutan N (ppm) masing-masing 54.86, 77.79, dan 76.28 serta memiliki konsentrasi NH3 (mg/L) masing-masing 66.61, 94.46, dan 92.63. Isolat bakteri pelarut fosfat (BPF9) memiliki indeks pelarutan fosfat sebesar 1.143. Masing-masing isolat bakteri penambat nitrogen kompatibel terhadap terhadap BPF9. Hasil menunjukkan populasi KBP2 dan KBP5 yang masing-masing dikonsorsiumkan dengan BPF 9 pada bahan pembawa biochar tongkol jagung memiliki populasi lebih tinggi (15.5 x 107 CFU/g) setelah 4 minggu penyimpanan. Kata kunci: penambat nitrogen, pelarut fosfat, bakteri konsorsium, biochar

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Advanced Molecular and Microspectroscopy Toolbox for Deciphering Soil Diazotroph Diversity

Kalia

Parshad

2013

Geomicrobiology and Biogeochemistry

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Ammonia Accumulation of Novel Nitrogen-Fixing Bacteria

Cited by 3 publications

References 13 publications

Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues

Using Cellulolytic Nitrogen Fixing Bacterium, Azomonas agilis for Effective Degradation of Agricultural Residues

Characteristic and viability of nitrogen fixation bacteria and phosphate solubilizing bacteria in carrier media

Advanced Molecular and Microspectroscopy Toolbox for Deciphering Soil Diazotroph Diversity

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