Ammonia exposure induces endoplasmic reticulum stress and apoptosis in Chinese striped-necked turtle (Mauremys sinensis)

Huang, Zubin; Liang, Lingyue; Li, Na; Li, Weihao; Zhang, Jiliang; Shi, Haitao; Ding, Li; Hong, Mingsheng

doi:10.1016/j.aquatox.2021.105903

Cited by 15 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ammonia is the main nitrogen-based catabolic product that is released in the aquaculture system. Recently, a high-density pattern emerged in the aquaculture industry, which has become the main trigger for the overproduction of ammonia nitrogen [ 18 ]. The high-fat diet feeding, and the ammonia nitrogen compounds, can induce oxidative stress by overproducing ROS, nitric oxide, and reactive nitrogen species in fish [ 19 ]; therefore, there is a pressing need to reveal the underlying physiological mechanisms of oxidative stress induced by high-fat diet feeding and ammonia nitrogen, and to develop antioxidant strategies.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the stocking density of spotted seabass is often high, with a production of >100 kg/m 3 ; thus, it is a good model to study the oxidative stress caused by high-fat or ammonia nitrogen. Furthermore, the stress of the endoplasmic reticulum (ERs) is often found in fish that are exposed to high-fat diets or ammonia nitrogen [ 13 , 16 , 18 , 22 ]. The ER is also an important source of ROS, which accounts for approximately 25% of all ROS produced [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Dong

Xia

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Oxidative stress is a common phenomenon in aquaculture, which can be induced by nutritional or environmental factors. Generally, oxidative stress causes poor growth performance, metabolic dysregulation, and even the death of aquatic animals. To identify a nutritional intervention strategy, high-fat diet (HFD) feeding (Experiment I) and acute ammonia nitrogen challenge (Experiment II) tests were carried out. In Experiment I, HFD feeding significantly decreased the growth performance concomitantly with excessive fat deposition in the liver and abdomen. The addition of 4-PBA in the diet improved the excessive fat accumulation. The activities of antioxidative enzymes were suppressed, and the levels of lipid and protein peroxidation were increased, indicating that HFD feeding induced oxidative stress. The endoplasmic reticulum stress (ERs) related genes were downregulated in the HFD group. Under a transmission electron microscope (TEM), more swollen and dilated ER lumen could be observed. These results indicated that the HFD induced ERs activation. Although 4-PBA acted as a potent ERs inhibitor, as evidenced by the alleviated alterations of ERs molecules and the ER ultrastructure, the oxidative stress was also attenuated by 4-PBA. In Experiment II, dietary 4-PBA improved the tolerance to the acute ammonia nitrogen challenge, as lower mortality and serum aminotransferase activity was found. Further results showed that 4-PBA decreased the peroxidation content and attenuated ERs, thus confirming the correlation between oxidative stress and ERs. Our findings showed that dietary 4-PBA supplementation can attenuate oxidative stress induced by a HFD or acute ammonia challenge; the mechanism is related to its potent inhibition effect for ERs.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Dong

Xia

et al. 2022

Antioxidants

View full text Add to dashboard Cite

show abstract

“…On the other hand, the release of pro-apoptotic members of the family, including Bax and cytc, will irreversibly activate downstream caspase to promote the process of apoptosis [47]. When M. sinensis was subjected to ammonia, the transcriptional levels of BAX and caspase-3 significantly upregulated, whereas Bcl-2 showed a distinct decrease, and the cleaved caspase-3 was also detected after 24 h of ammonia exposure [48]. In this experiment, with an increase in the concentration of BuP, the mRNA expression level of Bcl-2 in the liver gradually decreased, while the levels of Bax, cytc, caspase-3, and caspase-9 gradually increased, indicating that BuP could induce apoptosis via the caspase-dependent pathway in the hepatocytes of the turtle.…”

Section: Discussionmentioning

confidence: 99%

Effect of Butyl Paraben on Oxidative Stress in the Liver of Mauremys sinensis

Yin,

Xie,

Sun

et al. 2023

Toxics

Self Cite

View full text Add to dashboard Cite

Butyl paraben (BuP) has been widely used as a preservative in the cosmetics, food, and medicine industries. Recently, it has become a new pollutant and has attracted much attention. In order to evaluate the toxic effect of BuP on aquatic animals, Chinese striped-neck turtles (Mauremys sinensis) were exposed to BuP solutions with different concentrations of 0, 5, 50, 500, and 5000 µg/L for 20 weeks. The results showed that with an increase in BuP concentration, the activity of antioxidant enzymes (SOD, CAT and GSH-PX) in liver decreased. The expression of key genes in the Nrf2-Keap1 signal pathways first increased and then decreased, while the expression of the HSP70 and HSP90 genes increased. In addition, the liver had an inflammatory reaction. The expression of the BAFF and IL-6 genes increased and then decreased with an increase in BuP concentration, while the expression of P50 and P65 increased significantly. Oxidative stress induced apoptosis, and the expression of pro-apoptosis genes (BAX, cytc, Caspase3 and Caspase9) increased, while the expression of the anti-apoptosis gene Bcl2 decreased. The results provide an important reference for the comprehensive ecological and health risk assessment of environmental BuP.

show abstract

“…Toxic effects induced by ammonia exposure have been documented in various aquatic organisms [ 14 , 19 , 30 ]. Under exposure to higher concentrations of ammonia, animal growth was significantly reduced, which was observed in some fish species [ 31 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…As important aquaculture species, some freshwater turtles have been widely cultured in some countries. However, studies considering the impacts of aquaculture environmental pollutants, including ammonia-N, remain limited to only a few turtle species [ 10 , 17 , 18 , 19 , 20 , 21 ]. Specifically, the physiological responses and defense mechanisms to ammonia stress are relatively better understood only in the aquatic Chinese soft-shelled turtle, Pelodiscus sinensis [ 17 , 22 , 23 , 24 , 25 ], but not in other semi-aquatic turtle species.…”

Section: Introductionmentioning

confidence: 99%

Gut Microbial Composition and Liver Metabolite Changes Induced by Ammonia Stress in Juveniles of an Invasive Freshwater Turtle

Meng

Wang

et al. 2022

Biology

View full text Add to dashboard Cite

As the most common pollutant in aquaculture systems, the toxic effects of ammonia have been extensively explored in cultured fish, molluscs, and crustaceans, but have rarely been considered in turtle species. In this study, juveniles of the invasive turtle, Trachemys scripta elegans, were exposed to different ammonia levels (0, 0.3, 3.0, and 20.0 mg/L) for 30 days to evaluate the physiological, gut microbiomic, and liver metabolomic responses to ammonia in this turtle species. Except for a relatively low growth rate of turtles exposed to the highest concentration, ammonia exposure had no significant impact on the locomotor ability and gut microbial diversity of turtles. However, the composition of the microbial community could be altered, with some pathogenic bacteria being increased in ammonia-exposed turtles, which might indicate the change in their health status. Furthermore, hepatic metabolite profiles via liquid chromatography–mass spectrometry revealed extensive metabolic perturbations, despite being primarily involved in amino acid biosynthesis and metabolism. Overall, our results show that ammonia exposure causes gut dysbacteriosis and disturbs various metabolic pathways in aquatic turtle species. Considering discrepant defense mechanisms, the toxic impacts of ammonia at environmentally relevant concentrations on physiological performance might be less pronounced in turtles compared with fish and other invertebrates.

show abstract

Ammonia exposure induces endoplasmic reticulum stress and apoptosis in Chinese striped-necked turtle (Mauremys sinensis)

Cited by 15 publications

References 39 publications

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Effect of Butyl Paraben on Oxidative Stress in the Liver of Mauremys sinensis

Gut Microbial Composition and Liver Metabolite Changes Induced by Ammonia Stress in Juveniles of an Invasive Freshwater Turtle

Contact Info

Product

Resources

About