Ammonia half-saturation constants of sludge with different community compositions of ammonia-oxidizing bacteria

Kayee, Pantip; Rongsayamanont, Chaiwat; Kunapongkiti, Pattaraporn; Limpiyakorn, Tawan

doi:10.4491/eer.2015.121

Cited by 10 publications

(3 citation statements)

References 12 publications

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“…The ammonia half saturation constant of AOB was significantly increased from 0.7 to 26.6 mg N L −1 in accordance with the fact that the high ammonia levels in both SBRs allowed for selecting a biomass with low affinity for the substrate [44]. This was further confirmed by a specific microbiological characterisation of nitrifying bacteria that identified Nitrosomonas spp., which are characterized by high values of the ammonia half saturation constant, as the main AOB genera in both reactors (data to be published).…”

Section: Model Calibrationsupporting

confidence: 69%

Anammox-Based Processes for Mature Leachate Treatment in SBR: A Modelling Study

et al. 2021

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Mature landfill leachates are characterized by high levels of ammoniacal nitrogen which must be reduced for discharge in the sewer system and further treatment in municipal wastewater treatment plants. The use of anammox-based processes can allow for an efficient treatment of ammonium-rich leachates. In this work, two real scale sequencing batch reactors (SBRs), designed to initially perform partial nitritation/anammox (PN/A) and simultaneous partial nitrification and denitrification (SPND) for the treatment of ammonium-rich urban landfill leachate, were modelled using BioWin 6.0 in order to enable plant-wide modelling and optimizing. The constructed models were calibrated and validated using data from long- and short-term (one cycle) SBR operation and fit well to the main physical-chemical parameters (i.e., ammonium, nitrite and nitrate concentrations) measured during short-term (one cycle) operations. Despite the different strategies in terms of dissolved oxygen (DO) concentrations and aeration and mixing patterns applied for SBR operation, the models allowed for understanding that in both reactors the PN/A process was shown as the main contributor to nitrogen removal when the availability of organic carbon was low. Indeed, in both SBRs, the activity of nitrite oxidizing bacteria was inhibited due to high levels of free ammonia, whereas anammox bacteria were active due to the simultaneous presence of ammonium and nitrite and their ability to recover from DO inhibition. Increasing the external carbon addition, a prompt decrease of the anammox biomass was observed, with SPND becoming the main nitrogen removal mechanism. Models were also applied to estimate the production rates of nitrous oxide by aerobic ammonia oxidizing bacteria and heterotrophic denitrifiers. The models were found to be a robust tool for understanding the effects of different operating conditions (i.e, temperature, cycle phases, DO concentration, external carbon addition) on the nitrogen removal performances of the two reactors, assessing the contribution of the different bacterial groups involved.

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Section: Model Calibrationsupporting

confidence: 69%

Anammox-Based Processes for Mature Leachate Treatment in SBR: A Modelling Study

et al. 2021

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“…The K S was significantly higher than the typical values of half saturation coefficient (less than 1.0 mg N/L) [17]. It was reported that the K S of ammonia-oxidizing bacteria (AOB) could be in the range of 1-100 mg N/L, depending on the dominant group of microorganisms in the system [18]. However, the phylogenetic group of the mixed culture bacteria has not been identified in this study.…”

Section: Kinetics Of Nitrificationmentioning

confidence: 57%

Kinetics of nitrification and acrylamide biodegradation by <i>Enterobacter aerogenes</i> and mixed culture bacteria in sequencing batch reactor wastewater treatment systems

Madmanang¹,

Jangkorn²,

Charoenpanich³

et al. 2018

Environmental Engineering Research

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This study evaluated the kinetics of acrylamide (AM) biodegradation by mixed culture bacteria and Enterobacter aerogenes (E. aerogenes) in sequencing batch reactor (SBR) systems with AQUASIM and linear regression. The zero-order, first-order, and Monod kinetic models were used to evaluate the kinetic parameters of both autotrophic and heterotrophic nitrifications and both AM and chemical oxygen demand (COD) removals at different AM concentrations of 100, 200, 300, and 400 mg AM/L. The results revealed that both autotrophic and heterotrophic nitrifications and both AM and COD removals followed the Monod kinetics. High AM loadings resulted in the transformation of Monod kinetics to the first-order reaction for AM and COD removals as the results of the compositions of mixed substrates and the inhibition of the free ammonia nitrogen (FAN). The kinetic parameters indicated that E. aerogenes degraded AM and COD at higher rates than mixed culture bacteria. The FAN from the AM biodegradation increased both heterotrophic and autotrophic nitrification rates at the AM concentrations of 100-300 mg AM/L. At higher AM concentrations, the FAN accumulated in the SBR system inhibited the autotrophic nitrification of mixed culture bacteria. The accumulation of intracellular polyphosphate caused the heterotrophic nitrification of E. aerogenes to follow the first-order approximation.

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“…communis cluster have been classified as AOB with moderate affinity constants for ammonia (14 -43 µM). The member of N. oligotropha cluster, and N. oligotropha cluster are low in affinity constants for ammonia (1.9 -4.2 µM) was found in WWTP with low NH4 + -N load (10 mg N/ L) [11,92]. In industrial wastewater systems, AOB are often the main players in nitrification process [12].…”

Section: Ammonia Oxidizing Bacteria In Industrial Wwtpmentioning

confidence: 99%

Ammonia oxidation process of entrapped and suspended cell inhibited by silver nanoparticles

Giao

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Silver nanoparticles (AgNPs) are widely used in commercial products because of their excellent antimicrobial activity. Entrance of AgNPs and its released Ag ions (Ag+) into wastewater treatment plants could harm ammonia oxidation (AO) process resulting in environmental problems. This study aimed to study inhibitory effect of AgNPs and Ag+ on AO process in suspended cells of ammonia oxidizing cultures enriched at initial ammonia concentrations of 0.5 and 30 mM (called NAS 0.5 mM and NAS 30 mM, respectively) and then mitigation measures using entrapment technique were tested. The results indicated that AgNPs and Ag+ were higher toxic to NAS 0.5 mM than NAS 30 mM due to different proportion of active ammonia oxidizers and communities. Silver nanoparticles of 1-100 mg/L inhibited AO activity by 90.6 ± 8.6% to 94.8 ± 4.3% and 44.9 ± 2.4% to 73.8 ± 1.5%, whereas Ag+ concentration of 0.05- 0.50 mg/L inhibited by 86.3 ± 0.8% to 93.4 ± 1.24% and 52.7 ± 2.14% to 93.9 ± 1.89% for NAS 0.5 mM and NAS 30 mM, respectively. The inhibition result suggested that the AgNP toxicity mainly derived from the liberated Ag+. The primary mechanism for toxicity of AgNPs and Ag+ caused microbial cell death. Cell entrapped in barium alginate (BA), polyvinyl alcohol (PVA), and the mixture of PVA-BA used to mitigate negative influence of AgNPs and Ag+. The results showed that BA, PVA, and PVA-BA- entrapped cells remained AO 81-100%, 57-97%, and 75-100% at AgNPs 1-100 mg/L whereas the entrapped cells remained AO 98-100%, 61-99%, and 79-100% under Ag+ 0.05-0.50 mg/L, respectively. Less damage of membrane integrity in the entrapped cells experiments with AgNPs and Ag+ indicated that the entrapped cells were less exposed to silver since the entrapment matrices could limit mass transfer of silver into the gel beads. BA and PVA-BA were highly recommended for further study in the mitigating toxicity of AgNPs and Ag+ since they remained high cell viability and more stable in synthetic wastewater.

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Ammonia half-saturation constants of sludge with different community compositions of ammonia-oxidizing bacteria

Cited by 10 publications

References 12 publications

Anammox-Based Processes for Mature Leachate Treatment in SBR: A Modelling Study

Anammox-Based Processes for Mature Leachate Treatment in SBR: A Modelling Study

Kinetics of nitrification and acrylamide biodegradation by <i>Enterobacter aerogenes</i> and mixed culture bacteria in sequencing batch reactor wastewater treatment systems

Ammonia oxidation process of entrapped and suspended cell inhibited by silver nanoparticles

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