Ammonia permeability of the soybean nodulin 26 channel

Hwang, Jin Ha; Ellingson, Sally R.; Roberts, Daniel M.

doi:10.1016/j.febslet.2010.09.033

Cited by 70 publications

(47 citation statements)

References 24 publications

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“…Its detection in the PBS is unexpected as the C terminus of nodulin 26, to which glutamine synthetase binds, is cytosolic (92). The detection of both glutamine synthetase and nodulin 26 across all our samples, however, provides further support for their suggested roles in ammonia release from the symbiosome (16,20).…”

Section: Soybean Symbiosome Proteomementioning

confidence: 56%

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Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Clarke

Loughlin

Gavrin

et al. 2015

Molecular & Cellular Proteomics

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Legumes form a symbiosis with rhizobia in which the plant provides an energy source to the rhizobia bacteria that it uses to fix atmospheric nitrogen. This nitrogen is provided to the legume plant, allowing it to grow without the addition of nitrogen fertilizer. As part of the symbiosis, the bacteria in the infected cells of a new root organ, the nodule, are surrounded by a plant-derived membrane, the symbiosome membrane, which becomes the interface between the symbionts. Fractions containing the symbiosome membrane (SM) and material from the lumen of the symbiosome (peribacteroid space or PBS) were isolated from soybean root nodules and analyzed using nongel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to reduce complexity of the samples and enrich for hydrophobic integral membrane proteins. One hundred and ninety-seven proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and PBS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and degradation, membrane trafficking, and solute transport were identified. These included a number of proteins previously localized to the SM, such as aquaglyceroporin nodulin 26, sulfate transporters, remorin, and Rab7 homologs. Among the proteome were a number of putative transporters for compounds such as sulfate, calcium, hydrogen ions, peptide/ dicarboxylate, and nitrate, as well as transporters for which the substrate is not easy to predict. Analysis of the promoter activity for six genes encoding putative SM proteins showed nodule specific expression, with five showing expression only in infected cells. Localization of two proteins was confirmed using GFP-fusion experiments. The data have been deposited to the ProteomeXchange with identifier PXD001132. This proteome will provide a rich resource for the study of the legumerhizobium symbiosis. Molecular & Cellular Proteomics

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Section: Soybean Symbiosome Proteomementioning

confidence: 56%

“…There are two routes proposed for transport of fixed-N across the SM; as NH 3 through the aquaglyceroporin NOD26 (15,16) and as NH 4 ϩ through a monovalent cation channel (17). Although NOD26 is well described in soybean (16, 18 -21), the protein catalyzing monovalent cation transport has not been identified.…”

mentioning

confidence: 99%

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Clarke

Loughlin

Gavrin

et al. 2015

Molecular & Cellular Proteomics

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“…Aquaporins are integral membrane channels that facilitate the concentration gradient-driven water and/or solute transport through the plasma membrane. In root nodules, aquaporins have been shown to be involved in the NH4 + flux through the symbiosome membrane that encloses the N fixing bacteroids [135]. In ECM roots, they could facilitate the transport of NH4 + through the fungal plasma membrane into the interfacial apoplast and in combination with a reduced reabsorption increase the net transport of NH4 + to the host [118].…”

Section: Regulation Of Nutrient Transport Across the Mycorrhizal Intementioning

confidence: 99%

The Role of the Mycorrhizal Symbiosis in Nutrient Uptake of Plants and the Regulatory Mechanisms Underlying These Transport Processes

Bücking¹,

Liepold²,

Ambilwade³

2012

Plant Science

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“…Aquaporins are membrane proteins that facilitate the transport of small molecules such as water, glycerol, and ammonia (Kaldenhoff and Fischer, 2006;Chaumont and Tyerman, 2014). Higher plant aquaporins are subdivided into five main subfamilies: the plasma membrane intrinsic proteins, the tonoplast intrinsic proteins (TIPs), the Nodulin26-like intrinsic proteins, the small basic intrinsic proteins, and the X intrinsic proteins (Forrest and Bhave, 2007;Maurel et al, 2009;Wudick et al, 2009;Hwang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Adjustment of Host Cells for Accommodation of Symbiotic Bacteria: Vacuole Defunctionalization, HOPS Suppression, and TIP1g Retargeting in Medicago

et al. 2014

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In legume-rhizobia symbioses, the bacteria in infected cells are enclosed in a plant membrane, forming organelle-like compartments called symbiosomes. Symbiosomes remain as individual units and avoid fusion with lytic vacuoles of host cells. We observed changes in the vacuole volume of infected cells and thus hypothesized that microsymbionts may cause modifications in vacuole formation or function. To examine this, we quantified the volumes and surface areas of plant cells, vacuoles, and symbiosomes in root nodules of Medicago truncatula and analyzed the expression and localization of VPS11 and VPS39, members of the HOPS vacuole-tethering complex. During the maturation of symbiosomes to become N 2 -fixing organelles, a developmental switch occurs and changes in vacuole features are induced. For example, we found that expression of VPS11 and VPS39 in infected cells is suppressed and host cell vacuoles contract, permitting the expansion of symbiosomes. Trafficking of tonoplast-targeted proteins in infected symbiotic cells is also altered, as shown by retargeting of the aquaporin TIP1g from the tonoplast membrane to the symbiosome membrane. This retargeting appears to be essential for the maturation of symbiosomes. We propose that these alterations in the function of the vacuole are key events in the adaptation of the plant cell to host intracellular symbiotic bacteria.

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Ammonia permeability of the soybean nodulin 26 channel

Cited by 70 publications

References 24 publications

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

The Role of the Mycorrhizal Symbiosis in Nutrient Uptake of Plants and the Regulatory Mechanisms Underlying These Transport Processes

Adjustment of Host Cells for Accommodation of Symbiotic Bacteria: Vacuole Defunctionalization, HOPS Suppression, and TIP1g Retargeting in Medicago

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