Ammoniakentgiftung und Aminogruppenvorrat

Mothes, K.

doi:10.1007/978-3-642-94733-9_34

Cited by 16 publications

(2 citation statements)

References 240 publications

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“…carbohydrate) for the active processes involved in phloem translocation. A shortage in carbohydrates is indicated by the high increase in amides, particularly asparagine (Mothes 1958). Under conditions of low carbohydrate availability e.g.…”

Section: Discussionmentioning

confidence: 99%

Endopeptidase activity and nitrogen mobilization in senescing leaves of Nicotiana rustica in light and dark

Weckenmann

Martin

1984

Physiologia Plantarum

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The time course of endopeptidase activity (digestion of azocasein at pH 4.6) in leaves of intact plants of Nicotiana rustica L. was studied and related to changes in the contents of chlorophyll, total nitrogen and soluble and insoluble protein nitrogen. Endopeptidase activity increased several fold during senescence. However, the course of protein degradation did not reflect the steep slope of azocaseolytic activity. When single mature leaves were darkened, senescence proceeded faster than in illuminated leaves but the amount of nitrogen mobilized and translocated did not differ greatly between darkened and illuminated leaves. However, in contrast to leaves in light, azocaseolytic activity did not increase. Gelatin zymograms obtained using isoelectric focusing of extracts of mature leaves showed several bands in the pH 4.0 to 6.5 region of the gels. During senescence in both light and dark the position and number of bands remained largely unchanged. In leaves in light, the activity of endopeptidases focusing in the range pH 4.1 to 5.0 increased greatly. In leaves in dark, however, no major changes in activity could be detected. The results suggest that in tobacco leaves endopeptidase activity normally increases considerably during senescence but this increase is not a prerequisite for an effective protein degradation. Separation and analysis of free amino acids showed that during senescence in light the levels of all amino acids decreased considerably. In leaves senescing in the dark there were large increases in the levels of glutamine and asparagine, concomitant decreases in glutamate and aspartate, and considerable increases in all other amino acids.

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Section: Discussionmentioning

confidence: 99%

Endopeptidase activity and nitrogen mobilization in senescing leaves of Nicotiana rustica in light and dark

Weckenmann

Martin

1984

Physiologia Plantarum

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show abstract

“…In gro(?en, auch vom Standpunkt der Versuchsanstellung zufalligen Schwankungen des Asparaginanteils spiegelt sich der Einflufi verschiedener, zum Teil miteinander verkniipfter Faktoren wieder, wie Beleuchtung und Kohlensaureassimilation, Stickstoffversorgung und auch Infektionsdichte (vgl. SIEBERT 1961, RUDOLPH 1962, SHAW 1963; da im katabolischen Stoffwechsel freies Ammoniak in Asparagin bevorzugt festgelegt (LOOMIS und STUMPF 1958, MoTHES 1958 Summary 1. The influence of infection and chloramphenicol on primary leaves of wheat seedlings m water culture was investigated.…”

Section: Im Gegensatz Zu Der Relativen Unempfindlichkeit Der Beiden Punclassified

Weitere Untersuchungen über Möglichkeiten der Beeinflussung des Wirt-Parasit-Verhältnisses am Beispiel von Puccinia graminis tritici.

Blumenbach

1966

J Phytopathol

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Vergleichende Betrachtung des pflanzlichen Stoffwechsels

Mothes¹,

Reinbothe²,

Ohmann³

et al. 1966

Der Stoffwechsel

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Ammoniakentgiftung und Aminogruppenvorrat

Cited by 16 publications

References 240 publications

Endopeptidase activity and nitrogen mobilization in senescing leaves of Nicotiana rustica in light and dark

Endopeptidase activity and nitrogen mobilization in senescing leaves of Nicotiana rustica in light and dark

Weitere Untersuchungen über Möglichkeiten der Beeinflussung des Wirt-Parasit-Verhältnisses am Beispiel von Puccinia graminis tritici.

Vergleichende Betrachtung des pflanzlichen Stoffwechsels

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