Ammonium chloride: a novel effective and inexpensive salt solution for phycocyanin extraction from Arthrospira (Spirulina) platensis

Manirafasha, Emmanuel; Murwanashyaka, Theophile; Ndikubwimana, Theoneste; Qian, Yue; Zeng, Xianhai; Lu, Yinghua; Jing, Keju

doi:10.1007/s10811-016-0989-y

Cited by 36 publications

(26 citation statements)

References 36 publications

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“…C‐PC extraction from other strains of Spirulina by different extraction techniques has been reported in the literature . PEF‐assisted extraction of C‐PC led to an extraction yield of 159.9 mg/g and purity of 0.46 .…”

Section: Resultsmentioning

confidence: 99%

“…UAE of C‐PC obtained extraction yields of 86.3, 35.5, and 90.0 mg/g whereas purities were 1.32 and 0.65 . Four successive repeated freezing–thawing allowed the obtainment of purity around 1.4 . Biomass treated with ultrasonic bath and mechanical homogenizer provided yields of 98.1 and 68.1 mg/g, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Several cell rupture and C‐PC extraction methods from Spirulina have been reported, such as extraction assisted by pulsed electric field (PEF), freeze‐thawing, extraction assisted by mechanical homogenizer, ultrasound‐assisted extraction (UAE), ultrasonic bath extraction, microwave‐assisted extraction (MAE), milling, and supercritical fluid extraction, or the combination of these methods . C‐PC extraction yields of these methods ranged from 29.1 to 194 mg/g while purities varied from 0.46 to 1.4 . Regarding C‐PC from S. platensis LEB‐52 biomass, Moraes et al extracted it from wet biomass using an ultrasonic bath with glass pearls (extraction yield of 46.7 mg/g), whereas Moraes et al obtained C‐PC with purity of 0.60 and extraction yield of 82.5 mg/g from dried, frozen and milled biomass using water as the extraction solvent.…”

Section: Introductionmentioning

confidence: 99%

“…Several variables of cell rupture and/or extraction can affect C‐PC yield and purity. These variables include time, type of biomass (oven‐dry, freeze‐dry, or wet), solvent, temperature, biomass:solvent ratio, and agitation . Recently, Manirafasha et al showed that the use of 0.05 mmol/L ammonium chloride in substitution of sodium phosphate as extractor solvent during four successive repeated freezing–thawing increases C‐PC extract purity.…”

Section: Introductionmentioning

confidence: 99%

“…6 In order to obtain the dye, the cell must be ruptured before extraction. Several cell rupture and C-PC extraction methods from Spirulina have been reported, such as extraction assisted by pulsed electric field (PEF), 7 freeze-thawing, 4,8,9 extraction assisted by mechanical homogenizer, 10 ultrasound-assisted extraction (UAE), 5,[11][12][13][14][15] ultrasonic bath extraction, 10,16 microwave-assisted extraction (MAE), 10,13 milling, 17,18 and supercritical fluid extraction, 19 or the combination of these methods. 6 C-PC extraction yields of these methods ranged from 29.1 10 to 194 mg/g 4 while purities varied from 0.46 7 to 1.4.…”

Section: Introductionmentioning

confidence: 99%

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Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Sala

Moraes

Kalil

2018

Biotechnology Progress

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C‐phycocyanin (C‐PC) is a natural blue dye, and depending on its purity, which is measured by the ratio between the absorbance of the chromophore (A620) and the absorbance of the proteins (A280), it can be used in food (purity > 0.7), cosmetics (purity > 1.5), and therapeutic treatments (purity > 4.0). Several physical, chemical, and enzymatic methods of extraction are reported, however, few are able to extract C‐PC with purity above 0.7. An innovative method of C‐PC extraction with food grade purity from wet Spirulina platensis biomass is proposed. The cells were pretreated with ethylenediaminetetraacetic acid and subsequent C‐PC extraction was performed with tris‐(hydroxymethyl) aminomethane‐SO4 buffer. C‐PC was released after 12 h of cell pretreatment. Six variables of the extraction process were evaluated. The extraction temperature significantly influenced C‐PC extraction yield and purity. In the best condition of cell pretreatment and extraction, C‐PC with purity of 1.0 and extraction yield of 129.0 mg/g could be obtained to be used as a food dye without any purification process. Lastly, an ultrafiltration process was integrated and C‐PC was concentrated 8.8‐fold, resulting in purity of 1.6 and recovery of 93.4%.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Sala

Moraes

Kalil

2018

Biotechnology Progress

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Growth Inhibition of Microcystis aeruginosa by Copper‐based MOFs: Performance and Physiological Effect on Algal Cells

Fan

Zheng

Chen

2018

Applied Organom Chemis

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The problem of cyanobacteria bloom occurring in the eutrophic water increases more serious and urgent needs to be solved. Based on the advantages of Metal-organic frameworks (MOFs), some reported studies have evaluated the inhibition effect of MOFs on algae. However, the influence of that on the physiological characteristics of algal cells has not been investigated thoroughly.In this study, Cu-MOF-74 was successfully synthesized by a facile hydrothermal method and was characterized by XRD and SEM. The growth inhibition of Microcystis aeruginosa were conducted to explore the effects of Cu-MOF-74 based on the physiology of algal cells to reveal algae inhibition mechanism. The results showed that the synthesized Cu-MOF-74 was a large needle-shaped crystal, and the size of the grain was 3-4.5 μm. 1 mg/L of Cu-MOF-74 has relatively excellent inhibitory effect on algae, which can achieve the removal efficiency of 75.5% within 120 hr. Besides, the content of chlorophyll a decreased by about 56%, the diminishing of chlorophyll a and phycobiliprotein concentration resulted in photosystem damage. In this process, ROS accumulated continually in algal cells with the slow release of metal ions Cu 2+ of Cu-MOF-74 which acts as a 'reservoir', then causing the oxidation loss, the reduction of SOD activity and the increase of CAT activity. The hydroxyl radical generated by Cu-MOF-74 caused lipid peroxidation. Thereby the situation above eventually resulted in the death of Microcystis aeruginosa and the concentration of extracellular organic matter increased. All the above, we think Cu-MOF-74 can inhibit the growth of algal cells through effecting on physiology and can be applied to control the cyanobacteria blooms.

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Development of an efficient process to extract C‐phycocyanin from marine cyanobacterial strains and evaluation of its bioactive potential

Khazi,

Demirel,

Liaqat

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUNDPhycocyanin (PC), a natural blue photosynthetic pigment found in cyanobacteria, rhodophyta and cryptophyta has numerous applications in biotechnology and the food industry. The phycocyanin extraction method has a significant impact on its bioactivities, purity index, cost and potential applications; thus, the development of an efficient extraction strategy continues to be a primary research goal.RESULTSThis study evaluated the efficacy of different methods for the extraction of cyanobacterial phycocyanin (C‐PC) from three marine cyanobacterial isolates, namely Arthrospira platensis, Phormidium sp. and Pseudoscillatoria sp. wet biomass. The pretreatment step of soaking biomass in extraction buffer for 60 min, followed by a combination of freeze–thaw (one cycle; 2 h) and sonication (2 min) treatment was found to be the most effective extraction strategy, producing the highest C‐PC yield (16.19 ± 0.34%, 15.89 ± 0.61% and 15.56 ± 0.69% dry cell weight for strains A. platensis, Phormidium sp. and Pseudoscillatoria sp., respectively). Furthermore, a biomass‐to‐solvent ratio of 0.02 g mL−1 was optimal to attain food‐grade purity ((A620/A280) ≥ 0.7). Bioactivity evaluation of crude and purified C‐PC exhibited its potential as antioxidant compounds and as an antimicrobial agent with significant inhibitory effects against various pathogenic microorganisms.CONCLUSIONThe study proposed an efficient and cost‐effective C‐PC extraction process for cyanobacteria and highlighted the potential of C‐PC as a valuable bioactive compound with various applications. © 2024 Society of Chemical Industry (SCI).

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Ammonium chloride: a novel effective and inexpensive salt solution for phycocyanin extraction from Arthrospira (Spirulina) platensis

Cited by 36 publications

References 36 publications

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Growth Inhibition of Microcystis aeruginosa by Copper‐based MOFs: Performance and Physiological Effect on Algal Cells

Development of an efficient process to extract C‐phycocyanin from marine cyanobacterial strains and evaluation of its bioactive potential

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