Ammonium detoxification mechanism of ammonium-tolerant duckweed (Landoltia punctata) revealed by carbon and nitrogen metabolism under ammonium stress

Tian, Xueping; Fang, Yang; Jin, Yanling; Yi, Zhuolin; Li, Jinmeng; Du, Anping; He, Kai; Huang, Yuhong; Zhao, Hai

doi:10.1016/j.envpol.2021.116834

Cited by 36 publications

(36 citation statements)

References 58 publications

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“…All six species demonstrated an obvious preference for NH + 4 over NO − 3 when given the choice between the two under our experimental growth conditions. A similar N source preference was previously shown for at least two representative species of duckweed, Lemna gibba [76] and Landoltia punctata [36], which are also among the most tolerant of NH + 4 stress [27]. In our experiments the consumption of NO − 3 by the duckweeds did not start until the NH + 4 was exhausted (Figure 3).…”

Section: Duckweeds' Preference For Nh +supporting

confidence: 89%

“…However, some species, such as rice [33], demonstrate a preference for NH + 4 . A similar bias for NH + 4 over NO − 3 was shown for at least one duckweed species, dotted duckweed (Landoltia punctata) [36], which is also very tolerant to NH + 4 stress [27].…”

Section: Introductionsupporting

confidence: 68%

“…Plants 2022, 10, x FOR PEER REVIEW 3 of 22 least one duckweed species, dotted duckweed (Landoltia punctata) [36], which is also very tolerant to NH stress [27].…”

Section: Identity Of the Analyzed Speciesmentioning

confidence: 99%

“…Plant biomass accumulation is strongly associated with N utilization, and duckweed plants are extremely efficient at assimilating N. For example, duckweed nitrogen use efficiency (NUE) reached more than 68 kg biomass/kg N under N limitation due to N remobilization and recycling by the ubiquitin-proteasome system and autophagy [ 21 ]. However, despite intensive investigation of various duckweed species for remediation of wastewater and biomass production [ 22 , 23 , 24 , 25 ], studies of nutrient assimilation by duckweed species and the molecular mechanisms underlying duckweed’s remarkable NUE remain limited to a few recent studies [ 26 , 27 ]. By contrast, the major enzymes and molecular aspects of N assimilation have been uncovered in other plant species, primarily Arabidopsis thaliana and rice ( Oryza sativa ) [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

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The Dynamics of NO3− and NH4+ Uptake in Duckweed Are Coordinated with the Expression of Major Nitrogen Assimilation Genes

Zhou

Kishchenko

Stepanenko

et al. 2021

Plants

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Duckweed plants play important roles in aquatic ecosystems worldwide. They rapidly accumulate biomass and have potential uses in bioremediation of water polluted by fertilizer runoff or other chemicals. Here we studied the assimilation of two major sources of inorganic nitrogen, nitrate (NO3−) and ammonium (NH4+), in six duckweed species: Spirodela polyrhiza, Landoltia punctata, Lemna aequinoctialis, Lemna turionifera, Lemna minor, and Wolffia globosa. All six duckweed species preferred NH4+ over NO3− and started using NO3− only when NH4+ was depleted. Using the available genome sequence, we analyzed the molecular structure and expression of eight key nitrogen assimilation genes in S. polyrhiza. The expression of genes encoding nitrate reductase and nitrite reductase increased about 10-fold when NO3− was supplied and decreased when NH4+ was supplied. NO3− and NH4+ induced the glutamine synthetase (GS) genes GS1;2 and the GS2 by 2- to 5-fold, respectively, but repressed GS1;1 and GS1;3. NH4+ and NO3− upregulated the genes encoding ferredoxin- and NADH-dependent glutamate synthases (Fd-GOGAT and NADH-GOGAT). A survey of nitrogen assimilation gene promoters suggested complex regulation, with major roles for NRE-like and GAATC/GATTC cis-elements, TATA-based enhancers, GA/CTn repeats, and G-quadruplex structures. These results will inform efforts to improve bioremediation and nitrogen use efficiency.

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Section: Duckweeds' Preference For Nh +supporting

confidence: 89%

Section: Introductionsupporting

confidence: 68%

“…Plants 2022, 10, x FOR PEER REVIEW 3 of 22 least one duckweed species, dotted duckweed (Landoltia punctata) [36], which is also very tolerant to NH stress [27].…”

Section: Identity Of the Analyzed Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Dynamics of NO3− and NH4+ Uptake in Duckweed Are Coordinated with the Expression of Major Nitrogen Assimilation Genes

Zhou

Kishchenko

Stepanenko

et al. 2021

Plants

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show abstract

“…The stimulation of ammonium assimilation by simultaneously applied nitrate appears to explain the nitrate-mediated ammonium tolerance. A similar mechanism exists in duckweeds, as shown recently in Landoltia punctata [ 28 ]. A minor fraction of ammonium as the nitrogen source seemed to stimulate duckweed growth, while proportions of 50% and higher had a growth inhibiting effect.…”

Section: Discussionsupporting

confidence: 70%

Influence of the Nitrate-N to Ammonium-N Ratio on Relative Growth Rate and Crude Protein Content in the Duckweeds Lemna minor and Wolffiella hyalina

Petersen

Demann

Restemeyer

et al. 2021

Plants

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In order to produce protein-rich duckweed for human and animal consumption, a stable cultivation process, including an optimal nutrient supply for each species, must be implemented. Modified nutrient media, based on the N-medium for duckweed cultivation, were tested on the relative growth rate (RGR) and crude protein content (CPC) of Lemna minor and Wolffiella hyalina, as well as the decrease of nitrate-N and ammonium-N in the media. Five different nitrate-N to ammonium-N molar ratios were diluted to 10% and 50% of the original N-medium concentration. The media mainly consisted of agricultural fertilizers. A ratio of 75% nitrate-N and 25% ammonium-N, with a dilution of 50%, yielded the best results for both species. Based on the dry weight (DW), L. minor achieved a RGR of 0.23 ± 0.009 d−1 and a CPC of 37.8 ± 0.42%, while W. hyalina’s maximum RGR was 0.22 ± 0.017 d−1, with a CPC of 43.9 ± 0.34%. The relative protein yield per week and m2 was highest at this ratio and dilution, as well as the ammonium-N decrease in the corresponding medium. These results could be implemented in duckweed research and applications if a high protein content or protein yield is the aim.

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Are cysteine, glutathione and phytochelatins responses of Myriophyllum alterniflorum to copper and arsenic stress affected by trophic conditions?

et al. 2022

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Ammonium detoxification mechanism of ammonium-tolerant duckweed (Landoltia punctata) revealed by carbon and nitrogen metabolism under ammonium stress

Cited by 36 publications

References 58 publications

The Dynamics of NO3− and NH4+ Uptake in Duckweed Are Coordinated with the Expression of Major Nitrogen Assimilation Genes

The Dynamics of NO3− and NH4+ Uptake in Duckweed Are Coordinated with the Expression of Major Nitrogen Assimilation Genes

Influence of the Nitrate-N to Ammonium-N Ratio on Relative Growth Rate and Crude Protein Content in the Duckweeds Lemna minor and Wolffiella hyalina

Are cysteine, glutathione and phytochelatins responses of Myriophyllum alterniflorum to copper and arsenic stress affected by trophic conditions?

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