Ammonium uptake by heterotrophic bacteria in the Delaware estuary and adjacent coastal waters

Abstract

Uptake of NH4+ by heterotrophic bacteria and the relative importance of NH,+ and dissolved free amino acids (DFAA) as nitrogen sources for bacterial production were examined in the Delaware estuary and adjacent coastal waters during 1988 and 1990. Although total uptake of NH,+ and bacterial production were -4-fold higher in 1988 than in 1990, percent NH,+ uptake by bacteria in the upper and lower estuary was similar for both years. Bacterial uptake rates were highest at the mouth of the bay in summer and represented lo-25% of total uptake of NH,+. Less than 5% of NHd+ uptake was by bacteria at salinities <207& In contrast to NH4+ uptake, DFAA uptake was greatest in the upper estuary and often exceeded nitrogen requirements for bacterial growth. Bacteria accounted for 15-35% of total NH,+ uptake at coastal and offshore stations in 1990. About 50% of bacterial nitrogen demand was supported by NH4+ at the mouth of the bay and in coastal waters during summer, when DFAA concentrations were generally lowest. Although DFAA concentration and uptake did not explain all variability, they appeared to explain large-scale features in NH,+ uptake by heterotrophic bacteria. Ammonium uptake by bacteria was lowest in the estuary, where DFAA concentrations and uptake were highest; at an offshore station, where DFAA concentrations and uptake were low, relative NH,+ uptake by bacteria was highest. These and other results suggest that NH,+ uptake by bacteria is relatively high in oligotrophic water and low in eutrophic systems, which has important implications on the role of heterotrophic bacteria in the N cycling of marine environments.

Concentrations and uptake of neutral monosaccharides along 14°W in the equatorial Pacific: Contribution of glucose to heterotrophic bacterial activity and the DOM flux

“…1). Bacterial production somewhat followed primary production along the 12"N to 12"s transect, although there was much variation in the ratio of bacterial to primary production (Kirchman et al 1995).…”

“…Even though the ratio of bacterial production to primary production (BP:PP) is low in equatorial Pacific waters (-0.15 vs. the average of 0.30 for aquatic systems; Cole et al 1988; see also Ducklow and Carlson 1992), the labile DOM flux in these waters could still be substantial Carlson and Ducklow 1995) and perhaps is even greater north and south of the equator (Kirchman et al 1995). The few direct measures of this flux in any aquatic environments are restricted to DFAA.…”

“…The following conversion factors were used: 2.15 X 1 012 cells per mole of thymidine incorporated and 20 fg carbon per bacterial cell (Kirchman et al 1995). The depth profiles discussed by Kirchman et al (1995) were based on 8-10 depths, and bacterial production was based on both thymidine and leucine incorporation.…”

“…Details on the method were given by Kirchman et al (1995). The following conversion factors were used: 2.15 X 1 012 cells per mole of thymidine incorporated and 20 fg carbon per bacterial cell (Kirchman et al 1995).…”

“…In estuaries, DFAA seems to support a large fraction (if not all) of bacterial production (Billen and Fontigny 1987;Jorgensen et al 1993;Hoch and Kirchman 1995). Dissolved combined amino acids ' Corresponding author.…”