Ammonium Uptake by Rice Roots (I. Fluxes and Subcellular Distribution of 13NH4+)

Wang, M. Y.; Siddiqi, M. Yaeesh; Ruth, Thomas J.; Glass, Anthony D. M.

doi:10.1104/pp.103.4.1249

Cited by 160 publications

(178 citation statements)

References 25 publications

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“…This could be explained by each plant's specific and varietal characteristics, and by experimental conditions. Thus, there is a wide range of plant responses to NH 4 + nutrition; there are some species that are tolerant to high NH 4 + doses, such as rice (Wang et al, 1993), and some very sensitive species which practically cannot survive under NH 4 + nutrition, such as tomato or barley (Britto et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Ammonium enhances resistance to salinity stress in citrus plants

Fernández-Crespo

Camañes

García‐Agustín

2012

Journal of Plant Physiology

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In this work, we demonstrate that NH 4 + nutrition in citrange Carrizo plants acts as an inducer of resistance against salinity conditions. We investigated its mode of action and provide evidence that NH 4 + confers resistance by priming abscisic acid and polyamines, just as enhancing H 2 O 2 and proline basal content. Moreover it observed a diminished Cl -uptake as well as an enhanced PHGPx expression after salt stress. Control and N-NH 4 + plants have shown optimal growth, however it was observed that N-NH 4 + plants have displayed greater dry weight and total lateral roots than control plants, but that differences are not seen for primary roots length. Our results reveal that N-NH 4 + treatment induces a similar phenotypical response to the recent stress-induced morphogenetic response (SIMRs). The hypothesis is that N-NH 4 + treatment triggers mild chronic stress in citrange Carrizo plants, which might explain the SIMR observed. Moreover, we observed modulators of stress signaling, such as H 2 O 2 in N-NH 4 + plants, which could acts as an intermediary between stress and the development of the SIMR phenotype. This observation suggests that NH 4 + treatments induce a mild stress condition that primes the citrange Carrizo defense response by stress imprinting and confers protection against a subsequent salt stress.

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Section: Introductionmentioning

confidence: 99%

Ammonium enhances resistance to salinity stress in citrus plants

Fernández-Crespo

Camañes

García‐Agustín

2012

Journal of Plant Physiology

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“…4b). Carrier-mediated NH % + transport has been reported for green algae (Walker et al, 1979), liverworts (Felle, 1980), and higher plants (Ullrich et al, 1984 ;Wang et al, 1993Wang et al, , 1994, and an overview is given by Kleiner (1981). Green leaves of higher plants have hitherto not been well characterized, but because of the increasing pollution of the atmosphere with NH $ , such studies have become increasingly important (Nielsen & Schjoerring, 1998).…”

Section: Transport Of Nhmentioning

confidence: 99%

“…Thus we suggest that the cytoplasmic [NH % + ] in these leaves remains in the lower millimolar range. On the other hand, Wang et al (1993), applying compartmental analysis, came to estimates of 38 mM for rice roots grown in 1 mM NH % + ; the apparent higher affinity of the root transporter could be the reason for such high accumulations. It remains unclear whether NH % + transport in roots and leaves can be compared, and indeed there is evidence that transcripts of an NH % + transporter from tomato accumulated in roots, but not in shoots or leaves (Lauter et al, 1996).…”

Section: Transport Of Nhmentioning

confidence: 99%

The influence of atmospheric NH₃ on the apoplastic pH of green leaves: a non‐invasive approach with pH‐sensitive microelectrodes

Hanstein

Felle

1999

New Phytologist

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The apoplastic pH of intact green leaves of Bromus erectus was measured non-invasively by inserting blunt microelectrodes through stomatal openings. After making electrical contact, the recorded signal was stable for hours, yielding a pH of 4.67p0.10. The leaves responded to ' light-off ' with an initial transient acidification and subsequent sustained alkalinization of 0.2-0.3 pH ; ' light-on ' caused the opposite response. Flushing the leaves with 280 nmol NH $ mol −" air within 18p6 s alkalinized the apoplast by 0.22p0.07 pH, followed by a slower pH increase to reach a steady-state alkalinization of 0.53p0.14 after 19p7 min. This pH shift was persistent as long as the NH $ was flushed, and readily returned to its initial value after replacing the NH $ with clean air. The resultant [NH % + ] increase within the apoplast was measured with a NH % + -selective microelectrode. In the presence of 280 nmol NH $ mol −" air, apoplastic NH % + initially increased within 15p10 s to 1.53p0.41 mM, to reach a steady state of 1.62p0.16 mM after 27p7 min. An apoplastic buffer capacity of 6 mM pH −" unit was calculated from the initial changes of pH and [NH % + ], whereas the steady-state values yielded 2.7 mM pH −" . Infiltrated leaves responded to NH % + with concentration-dependent depolarizations, the maxima of which yielded saturation kinetics indicating carrier-mediated NH % + uptake into adjacent cells, as well as a linear component indicating nonspecific transport. We infer that the initial alkalinization is due to rapid conversion of NH $ to NH % + , whereas the slower pH increase would be caused by regulatory processes involving both membrane transport, and (mainly) NH % + assimilation. Possible consequences of the NH $ -induced pH shift for the development of plants growing in polluted areas are discussed.

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“…Clearly, N-use efficiency in rice is of key concern. In sharp contrast to most agricultural soils, where nitrate (NO $ − ) is the predominant N species (Kronzucker et al, 1998), hypoxic conditions in the paddy environment largely preclude the microbial formation of NO $ − through nitrification (Bouldin, 1986 ;Arth et al, 1998) and, consequently, ammonium (NH % + ) is the main form of N available to rice in the field (Shen, 1969 ;Wang et al, 1993 ;Arth et al, 1998 ;Kronzucker et al, 1998). It is therefore not surprising that NH % + nutrition, as opposed to NO $ − nutrition, has received almost exclusive attention in rice (Bonner, 1946 ;Fried et al, 1965 ;Shen, 1969 ;Wang et al, 1993).…”

Section: mentioning

confidence: 99%

“…In sharp contrast to most agricultural soils, where nitrate (NO $ − ) is the predominant N species (Kronzucker et al, 1998), hypoxic conditions in the paddy environment largely preclude the microbial formation of NO $ − through nitrification (Bouldin, 1986 ;Arth et al, 1998) and, consequently, ammonium (NH % + ) is the main form of N available to rice in the field (Shen, 1969 ;Wang et al, 1993 ;Arth et al, 1998 ;Kronzucker et al, 1998). It is therefore not surprising that NH % + nutrition, as opposed to NO $ − nutrition, has received almost exclusive attention in rice (Bonner, 1946 ;Fried et al, 1965 ;Shen, 1969 ;Wang et al, 1993). However, some reports have indicated that rice does possess some capacity for root NO $ − absorption (Ismunadji & Dijkshoorn, 1971 ;Sasakawa & Yamamoto, 1978 ;Youngdahl et al, 1982 ;Raman et al, 1995) and for the reduction of NO $ − in leaves (Tang & Wu, 1957).…”

Section: mentioning

confidence: 99%

Comparative kinetic analysis of ammonium and nitrate acquisition by tropical lowland rice: implications for rice cultivation and yield potential

et al. 2000

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Nitrogen limitation compromises the realization of yield potential in cereals more than any other single factor. In rice, the world's most important crop species, the assumption has long been that only ammonium-N is efficiently utilized. Consequently, nitrate utilization has been largely ignored, although fragmentary data have suggested that growth could be substantial on nitrate. Using the short-lived radiotracer "$N, we here provide direct comparisons of root transmembrane fluxes and cytoplasmic pool sizes for nitrate-and ammonium-N in a major variety of Indica rice (Oryza sativa), and show that nitrate acquisition is not only of high capacity and efficiency but is superior to that of ammonium. We believe our results have implications for rice breeding and molecular genetics as well as the design of water-management and fertilization regimes. Potential strategies to harness this hitherto unexplored N-utilization potential are proposed.

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Ammonium Uptake by Rice Roots (I. Fluxes and Subcellular Distribution of 13NH4+)

Cited by 160 publications

References 25 publications

Ammonium enhances resistance to salinity stress in citrus plants

Ammonium enhances resistance to salinity stress in citrus plants

The influence of atmospheric NH₃ on the apoplastic pH of green leaves: a non‐invasive approach with pH‐sensitive microelectrodes

Comparative kinetic analysis of ammonium and nitrate acquisition by tropical lowland rice: implications for rice cultivation and yield potential

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Ammonium Uptake by Rice Roots (I. Fluxes and Subcellular Distribution of 13NH4+)

Cited by 160 publications

References 25 publications

Ammonium enhances resistance to salinity stress in citrus plants

Ammonium enhances resistance to salinity stress in citrus plants

The influence of atmospheric NH3 on the apoplastic pH of green leaves: a non‐invasive approach with pH‐sensitive microelectrodes

Comparative kinetic analysis of ammonium and nitrate acquisition by tropical lowland rice: implications for rice cultivation and yield potential

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The influence of atmospheric NH₃ on the apoplastic pH of green leaves: a non‐invasive approach with pH‐sensitive microelectrodes