Ammonium Vanadate Decreases Viability and Proliferation Activity of Cultured Virustransformed Rat Sarcoma Cells

Abstract

The aim of the study presented was to evaluate the effect of ammonium vanadate (NH 4 VO 3 ) on the viability and proliferation of cultured virustrasformed rat sarcoma LSR-SF-SR cells. The investigations were performed by MTT test, neutral red uptake cytotoxicity assay, double staining with acridine orange and propidium iodide, the method of Pappenheim and colony-forming technique. The results obtained revealed that applied at a concentration range of 0.1-20 µg/ml NH 4 VO 3 expresses significant cytotoxic and/or cytostatic effects that are time-and concentration-dependent.

Homo- and heterometallic complexes of Zn(ii), {Zn(ii)Au(i)}, and {Zn(ii)Ag(i)} with pentadentate Schiff base ligands as promising anticancer agents

“…The potential of the investigated compounds to inhibit the ability of the treated cancer cells to form 3D colonies in a semi-solid medium was evaluated by the colony-forming method. 54 Tumor cells (approximately 10 3 cells per well) suspended in 0.45% purified agar in 2× D-MEM medium containing different concentrations of the compounds examined (ranging from 0.1 to 200 μg mL −1 ) were layered in 24-well plates. The presence or absence of 3D cell colonies was registered using an inverted microscope (Carl Zeiss, Jena, Germany) over 28 days.…”