Amnion Cells in Tailored Hydrogels Deposit Human Amnion Native Extracellular Matrix

Avilla-Royo, Eva; Roschitzki, Bernd; Pfammatter, Sibylle; Grossmann, Jonas; Airaghi, P.; Vonzun, Ladina; Ochsenbein‐Kölble, Nicole; Vallmajó-Martín, Queralt; Ehrbar, Martin

doi:10.1002/adfm.202204543

Cited by 3 publications

(2 citation statements)

References 78 publications

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“…Type I collagen accelerated the regeneration of the ruptured amnion, as verified by the upregulation of E-cadherin and 𝛼-smooth muscle actin (𝛼-SMA) expression (Figure 2f-g and Figure S7, Supporting Information). [23,24] Amniotic mesenchymal cells remodel amnion surroundings by secreting abundantly amnion proteins, including transforming growth factor 𝛽induced protein ig-h3 (TGF-𝛽i), [25] which is a very distinctive amnion protein involved in the wound healing cascade. Immunofluorescence results revealed that the accumulation of TGF-𝛽i increased at the implant sites after the IEFS implantation.…”

Section: Resultsmentioning

confidence: 99%

Interface‐Stabilized Fiber Sensor for Real‐Time Monitoring of Amniotic Fluid During Pregnancy

Li,

et al. 2023

Advanced Materials

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Diseases in pregnancy endanger millions of fetuses worldwide every year. The onset of these diseases can be early warned by the dynamic abnormalities of biochemicals in amniotic fluid, thus requiring real‐time monitoring. However, when continuously penetrated by detection devices, the amnion is prone to loss of robustness and rupture, which is difficult to regenerate. Here, we present an interface‐stabilized fiber sensor for real‐time monitoring of biochemical dynamics in amniotic fluid during pregnancy. The sensor is seamlessly integrated into the amnion through tissue adhesion, amniotic regeneration and uniform stress distribution, posing no risk to the amniotic fluid environment. The sensor demonstrates a response performance of less than 0.3% fluctuation under complex dynamic conditions and an accuracy of more than 98% from the second to the third trimester. By applying it to early warning of diseases such as intrauterine hypoxia, intrauterine infection, and fetal growth restriction, fetal survival increases to 95% with timely intervention.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Interface‐Stabilized Fiber Sensor for Real‐Time Monitoring of Amniotic Fluid During Pregnancy

Li,

et al. 2023

Advanced Materials

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“…Independently of this, our results speak in favor of finding alternative solutions and/or modifying the glue composition to minimize diffusivity and optimize adhesion. Recent research shows that biomaterials based on synthetic backbone with highly predictable mechanical properties [30,31], and strong adhesives adapted for wet environments [20,[32][33][34], could have the potential to strongly glue to the FMs but also trigger cell mobilization for tissue healing provided the right factors are delivered [35][36][37]. Our application device and umbrella receptor could be combined to those alternative materials as a strategy for a controlled delivery of the optimal material.…”

Section: Discussionmentioning

confidence: 99%

Tissue Glue-Based Sealing Patch for the in vivo Prevention of Iatrogenic Prelabor Preterm Rupture of Fetal Membranes

Devaud,

Avilla-Royo,

Lionetti

et al. 2023

Fetal Diagn Ther

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Introduction: One of the main concerns for all fetal surgeries is the risk of preterm delivery due to the preterm prelabor rupture of the fetal membranes (iPPROM). Clinical approaches to seal fetal membrane (FM) defects are missing due to the lack of appropriate strategies to apply sealing biomaterials at the defect site. Methods: Here, we test the performance of a previously developed strategy to seal FM defects with cyanoacrylate-based sealing patches in an ovine model up to 24 days after application. Results: Patches sealed tightly the fetoscopy-induced FM defects and remained firmly attached to the defect over 10 days. At 10 days after treatment, 100% (13/13) of the patches were attached to the FMs, and 24 days after treatment 25% (1/4) of the patches placed in CO2 insufflation, and 33% (1/3) in NaCl infusion remained. However, all successfully applied patches (20/24) led to a watertight sealing at 10 or 24 days after treatment. Histological analysis indicated that cyanoacrylates induced a moderate immune response and disrupted the FM epithelium. Conclusion: Together, these data show the feasibility of minimally invasive sealing of FM defects by locally gathering tissue adhesive. Further development to combine this technology with refined tissue glues or healing-inducing materials holds great promise for future clinical translation.

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Engineered Platelet‐Derived Growth Factor‐Releasing Hydrogels Promote Fetal Membrane Healing In Vivo

Avilla-Royo

Vonzun

Seehusen

et al. 2023

Adv Funct Materials

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Fetoscopic interventions to treat fetal anomalies are currently performed for a variety of conditions. Depending on the procedure, preterm rupture of the fetal membranes (FMs) happens in around 30% of the cases, potentially leading to preterm birth and fetal morbidity and mortality. Here, the capacity of modular transglutaminase crosslinked poly(ethylene glycol) (TG-PEG) hydrogels that release platelet-derived growth factor (PDGF)-BB to promote FM healing is described. In vitro, such growth factor-loaded hydrogels are able to stimulate amniotic cell migration and proliferation. When applied in vivo, these TG-PEG hydrogels tightly seal the FM and uterus defects created by a fetoscope and remain stable for 10 days. The migration of healing-related cells into such hydrogels in the myometrium, endometrium, and FM areas is only possible in soft TG-PEG hydrogels. Importantly, bioengineered hydrogels releasing PDGF-BB promote recruitment of host cells from the myometrium and the endometrium, and to a lesser extent from FM areas. In such hydrogels, the potent proliferation and matrix production of the recruited cells at the site of treatment into the biomaterial initiates a robust early healing response. PDGF-BB-loaded TG-PEG hydrogels hold great promise for the treatment of fetoscopy-induced FM defects and for the prevention of preterm birth.

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Amnion Cells in Tailored Hydrogels Deposit Human Amnion Native Extracellular Matrix

Cited by 3 publications

References 78 publications

Interface‐Stabilized Fiber Sensor for Real‐Time Monitoring of Amniotic Fluid During Pregnancy

Interface‐Stabilized Fiber Sensor for Real‐Time Monitoring of Amniotic Fluid During Pregnancy

Tissue Glue-Based Sealing Patch for the in vivo Prevention of Iatrogenic Prelabor Preterm Rupture of Fetal Membranes

Engineered Platelet‐Derived Growth Factor‐Releasing Hydrogels Promote Fetal Membrane Healing In Vivo

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