Amniotic fluid Interleukin-8 as a marker for intraamniotic infection

Puchner, T.; Egarter, Christian; Wimmer, C; Lederhilger, F; Weichselbraun, I

doi:10.1007/bf02770627

Cited by 37 publications

(12 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16.0, 14.0Ϫ33.1 pg/ml vs. 13.2, 10.6Ϫ 17.6 pg/ml in non infection group, p < 0.05). No significant difference was noticed in cord blood concentrations of G-CSF and IL-8 during histologic chorioamnionitis or in early-onset neonatal…”

mentioning

confidence: 84%

See 1 more Smart Citation

Il-1β, Il-6, Il-8 and G-CSF in the diagnosis of early-onset neonatal infections

Büscher¹,

Chen²,

Pitzen³

et al. 2000

Journal of Perinatal Medicine

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 84%

“…Amniotic fluid levels have been shown to predict vascular extension of chorioamnionitis and neonatal infection [1,3,5,15,16,21,25]. Amniotic fluid levels have been shown to predict vascular extension of chorioamnionitis and neonatal infection [1,3,5,15,16,21,25].…”

Section: Introductionmentioning

confidence: 99%

Il-1β, Il-6, Il-8 and G-CSF in the diagnosis of early-onset neonatal infections

Büscher¹,

Chen²,

Pitzen³

et al. 2000

Journal of Perinatal Medicine

View full text Add to dashboard Cite

show abstract

“…Similar to IL-6, elevated IL-8 levels have also been associated with clinical or histological signs of intrauterine infection 61 (Table 3 [B]). IL-8 is significantly increased during labor, but even higher values have been detected when labor was associated with positive amniotic fluid cultures 59 (Table 3 [B]).…”

Section: Amniotic Fluidmentioning

confidence: 99%

Intrapartum Fever at Term

Evers¹,

Nijhuis²,

Koster³

et al. 2012

Obstetrical &Amp; Gynecological Survey

View full text Add to dashboard Cite

Intrauterine infection is a serious complication during labor at term and is associated with adverse neonatal outcome. Early and accurate diagnosis is of great concern for both obstetrician and pediatrician with the use of current diagnostics. Clinical symptoms are often regarded as the main sign of intrauterine infection but this approach is highly unreliable and leads to both underand overtreatment. Currently, no distinct fetal heart rate (FHR) patterns have been found that reliably identify neonates with intrauterine infection. Using a systematic literature search, this article reviews possible markers for the early detection of intrauterine or neonatal infection in maternal serum, amniotic fluid, and umbilical cord blood during labor at term. Maternal serum markers, with the possible exception of interleukin (IL)-8, are unreliable for the detection of intrauterine infection. In contrast, amniotic fluid levels of especially IL-6 and IL-8 are significantly associated with intrauterine infection. Umbilical cord blood IL-6 has been extensively investigated and is usually elevated in case of intrauterine or neonatal infection but shows only modest positive and negative predictive values (NPVs) for clinical use. Umbilical cord IL-8 concentration could be a valuable addition in the diagnostic process, as it has shown to have an NPV of 84% to 92% in the detection of neonatal infection and histological chorioamnionitis. Future research is essential and should focus on the combination of different markers and on the development of a prediction model, to improve the positive and NPVs of our arsenal to detect intrauterine and neonatal infections. Amniotic fluid and umbilical cord values of IL-6 and IL-8 levels are likely candidates for such a prediction model. Target Audience: Obstetricians & Gynecologists and Family PhysiciansLearning Objectives: After the completing the CME activity, physicians should be better able to evaluate the use of clinical chorioamnionitis with regard to histological evidence and as a diagnostic tool in early diagnosis of intra-amniotic infection. Asses the use of amniotic fluid IL-6 and IL-8 as diagnostic tools to detect early intra-amniotic infection and assess umbilical cord blood IL-8 in case of intrauterineor neonatal infection using positive (PPV) and negative predictive values (NPV).

show abstract

“…[8] The positive correlation between maternal and cord serum IL-8 levels was found to be useful for rapid prenatal screening of chorioamnionitis at term. [8] The positive correlation between maternal and cord serum IL-8 levels was found to be useful for rapid prenatal screening of chorioamnionitis at term.…”

Section: Discussionmentioning

confidence: 99%

“…Interleukin 8 (IL-8), a CXC chemokine produced by various types of cells including monocytes/macrophages, eosinophils, T-cells, NK cells, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes, chondrocytes, and neutrophils upon stimulation with proinflammatory stimuli, exerts a variety of functions particularly on leukocytes. [8,9] The IL-8 molecule was originally reported as 10 kDa protein but later precise determinations have demonstrated that natural IL-8 occurs as a 6-8 kDa doublet. [4] The IL-8 levels in different body fluids very often correlate with the severity of the diseases, as found in bronchoalveolar lavage of patients with acute respiratory distress syndrome, [5] in plasma of patients with multiple organ failure, [6,7] and in amniotic fluid during intraamniotic infections.…”

Section: Introductionmentioning

confidence: 99%

Development and Application of a Pig IL-8 ELISA Detection System

Šplı́chal¹,

Muneta²,

Mori³

et al. 2003

Journal of Immunoassay and Immunochemistry

View full text Add to dashboard Cite

Interleukin 8 (IL-8) is a chemotactic and activating chemokine, especially for neutrophils, which plays an important role in inflammatory process. A pig IL-8 specific enzyme-linked immunosorbent assay (ELISA) was developed to measure IL-8 concentrations in cell culture supernatants and biological fluids. A streptavidin-biotin amplified sandwich method uses mouse capture mAb IZ8.03 and detection biotinylated mouse mAb IZ8.04 against recombinant pig IL-8. The assay specifically and reproducibly recognizes both recombinant and natural pig IL-8. A working range of the assay is 16-1000 pg/mL and takes a mere 3.5 h of incubation time. This pig IL-8 ELISA is a suitable alternative way of measurement of IL-8 concentrations to time consuming and laborious IL-8 bioassays.

show abstract

Amniotic fluid Interleukin-8 as a marker for intraamniotic infection

Cited by 37 publications

References 12 publications

Il-1β, Il-6, Il-8 and G-CSF in the diagnosis of early-onset neonatal infections

Il-1β, Il-6, Il-8 and G-CSF in the diagnosis of early-onset neonatal infections

Intrapartum Fever at Term

Development and Application of a Pig IL-8 ELISA Detection System

Contact Info

Product

Resources

About