Amniotic Fluid Stem-Cell Extracellular Vesicles Improve Pulmonary Vasculature in Experimental Congenital Diaphragmatic Hernia via the Release of RNA Cargo

Antounians, Lina; Catania, Vincenzo Davide; Belfiore, Alyssa; Pellerito, Ornella; Montalva, Louise; Zani, Augusto

doi:10.1016/j.jamcollsurg.2018.07.423

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One the most challenging aspects of pulmonary hypoplasia is its associated remodeling of the pulmonary vasculature. Our preliminary results show that administration of AFSC‐EVs to fetal rats with CDH improves markers of vascular development, rescues the number of pulmonary vessels, and decreases the arterial medial wall thickness 72,73 . Moreover, we identified some miRNAs that are contained in the AFSC‐EVs and that could be responsible for the effects on pulmonary vasculature 72 .…”

Section: Ev Effects On Pulmonary Vascular Developmentmentioning

confidence: 76%

“…Our preliminary results show that administration of AFSC-EVs to fetal rats with CDH improves markers of vascular development, rescues the number of pulmonary vessels, and decreases the arterial medial wall thickness. 72,73 Moreover, we identified some miRNAs that are contained in the AFSC-EVs and that could be responsible for the effects on pulmonary vasculature. 72 75 All these studies indicate the potential of an EV-based therapy to address prenatal vascular remodeling and prevent the postnatal development of pulmonary hypertension.…”

Section: Ev Effects On Pulmonary Vascular Developmentmentioning

confidence: 99%

“…72,73 Moreover, we identified some miRNAs that are contained in the AFSC-EVs and that could be responsible for the effects on pulmonary vasculature. 72 75 All these studies indicate the potential of an EV-based therapy to address prenatal vascular remodeling and prevent the postnatal development of pulmonary hypertension.…”

Section: Ev Effects On Pulmonary Vascular Developmentmentioning

confidence: 99%

See 2 more Smart Citations

Fetal lung regeneration using stem cell‐derived extracellular vesicles: A new frontier for pulmonary hypoplasia secondary to congenital diaphragmatic hernia

et al. 2022

Self Cite

View full text Add to dashboard Cite

The poor outcomes of babies with congenital diaphragmatic hernia (CDH) are directly related to pulmonary hypoplasia, a condition characterized by impaired lung development. Although the pathogenesis of pulmonary hypoplasia is not fully elucidated, there is now evidence that CDH patients have missing or dysregulated microRNAs (miRNAs) that regulate lung development. A prenatal therapy that supplements these missing/dysregulated miRNAs could be a strategy to rescue normal lung development. Extracellular vesicles (EVs), also known as exosomes when of small dimensions, are lipid‐bound nanoparticles that can transfer their heterogeneous cargo (proteins, lipids, small RNAs) to target cells to induce biological responses. Herein, we review all studies that show evidence for stem cell‐derived EVs as a regenerative therapy to rescue normal development in CDH fetal lungs. Particularly, we report studies showing that administration of EVs derived from amniotic fluid stem cells (AFSC‐EVs) to models of pulmonary hypoplasia promotes fetal lung growth and maturation via transfer of miRNAs that are known to regulate lung developmental processes. We also describe that stem cell‐derived EVs exert effects on vascular remodeling, thus possibly preventing postnatal pulmonary hypertension. Finally, we discuss future perspectives and challenges to translate this promising stem cell EV‐based therapy to clinical practice.

show abstract

Section: Ev Effects On Pulmonary Vascular Developmentmentioning

confidence: 76%

Section: Ev Effects On Pulmonary Vascular Developmentmentioning

confidence: 99%

Section: Ev Effects On Pulmonary Vascular Developmentmentioning

confidence: 99%

See 1 more Smart Citation

Fetal lung regeneration using stem cell‐derived extracellular vesicles: A new frontier for pulmonary hypoplasia secondary to congenital diaphragmatic hernia

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Fetal therapy for congenital diaphragmatic hernia: past, present and future

Abbasi,

Mieghem,

Ryan

2024

World Jnl Ped Surgery

View full text Add to dashboard Cite

Congenital diaphragmatic hernia (CDH) affects 1/2500-5000 infants and is associated with significant neonatal morbidity and mortality related to pulmonary hypoplasia and pulmonary hypertension. Current estimates of perinatal mortality are between 30-40%. With advances in neonatal and surgical management and now improvements in prenatal diagnosis and intervention, further reduction in mortality is anticipated. Data from the international Tracheal Occlusion to Accelerate Lung Growth (TOTAL) trials, have demonstrated the efficacy of fetal endoscopic tracheal occlusion (FETO) in severe left CDH (LCDH). Although promising, this intervention also has potential for significant morbidity related to prematurity and iatrogenic mortality if reversal of tracheal occlusion is unsuccessful. The implementation of FETO must proceed cautiously within Level III fetal therapy centers and with rigorous outcomes monitoring of centers offering this therapy, ensuring that they are experienced in antenatal severity assessment of CDH, FETO insertion and removal and are integrated with expert, standardized neonatal CDH centers with availability of Extracorporeal life support (ECLS). Further research is needed to better understand the impact of prematurity on FETO survivors, the role of FETO in moderate LCDH, Right CDH (RCDH) and non-isolated CDH in carefully selected circumstances as well as the development of alternative, less invasive, fetal therapies that can specifically target both pulmonary hypoplasia and pulmonary hypertension.

show abstract

Amniotic Fluid Stem-Cell Extracellular Vesicles Improve Pulmonary Vasculature in Experimental Congenital Diaphragmatic Hernia via the Release of RNA Cargo

Cited by 2 publications

References 0 publications

Fetal lung regeneration using stem cell‐derived extracellular vesicles: A new frontier for pulmonary hypoplasia secondary to congenital diaphragmatic hernia

Fetal lung regeneration using stem cell‐derived extracellular vesicles: A new frontier for pulmonary hypoplasia secondary to congenital diaphragmatic hernia

Fetal therapy for congenital diaphragmatic hernia: past, present and future

Contact Info

Product

Resources

About