Amniotic Membrane Transplantation With or Without Limbal Allografts for Corneal Surface Reconstruction in Patients With Limbal Stem Cell Deficiency

Tseng, Scheffer C.G.; Prabhasawat, Pinnita; Barton, Keith; Gray, Trevor; Meller, Daniel

doi:10.1001/archopht.116.4.431

Cited by 633 publications

(400 citation statements)

References 20 publications

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“…The amnion along with the underlying chorion was cut up into pieces measuring 5 Â 5 cm 2 in size and stored in normal saline containing 50,000 U of penicillin and 1 g of streptomycin per 400 ml of saline, stored at 4 1 C not exceeding a period of 48 h. At the time of surgery, the amnion was dissected bluntly from the chorion, and washed thoroughly in normal saline containing gentamicin prior to use. In six eyes (1,5,7,8,14,15), amniotic membrane was used as a circular patch covering the cornea and limbus and sutured to the less damaged conjunctival surface (so that the damaged area was covered). In the remaining nine eyes, amniotic membrane covered the whole ocular surface from lid margin to lid margin.…”

Section: Methodsmentioning

confidence: 99%

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Amniotic membrane transplantation in acute chemical burns

Arora

Mehta

Jain

2004

Eye

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Section: Methodsmentioning

confidence: 99%

“…One eye (4) with Grade II burn developed minimal inferior forniceal shortening. One eye (15) in Grade III burns developed mild symblepharon. In all, 7 out of 8 eyes with grade IV burns developed symblepharon.…”

Section: Eyementioning

confidence: 99%

Amniotic membrane transplantation in acute chemical burns

Arora

Mehta

Jain

2004

Eye

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“…Briefly, human placenta was collected at elective cesarean delivery (Heiligenhaus et al, 2001;Lee and Tseng, 1997;Prabhasawat et al, 1997;Tseng et al, 1998). The amniotic membrane was flattened onto nitrocellulose paper (Hybond N+, Amersham, UK), with the epithelium surface up.…”

Section: Preparation Of Preserved Human Amniotic Membranementioning

confidence: 99%

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

Bauer

Wasmuth

Hermans

et al. 2007

Experimental Eye Research

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Necrotizing herpetic stromal keratitis (HSK) in mice rapidly improved after amniotic membrane transplantation (AMT). In this study we determined the fate of polymorphonuclear neutrophils (PMN) after AMT. AMT or tarsorrhaphy (T) was performed in BALB/c mice with ulcerative HSK. After 2 days, corneas were studied histologically and by transmission electron microscopy (TEM). CD11b, Gr-1, and TUNEL-positive cells were identified. Macrophages were depleted by subconjunctival injection of dichloromethylene-diphosphonate-liposomes (Cl 2 MDP-LIP) before AMT. Corneas were studied for interleukin (IL)-1α, IL-2, interferon (IFN)-γ, CXCL1, CXCL2, and tumor necrosis factor (TNF)-α production by ELISA. PMN-enriched cell preparations cocultured with amniotic membrane (AM) or with AM and such recombinant (r) cytokines as rIL-1α, rIL-2, and rTNF-α or supernatants from activated lymphocytes were investigated by flow cytometry (Annexin-V/7-AAD and TUNEL), and a dimethylthiazolyl-diphenyltetrazoliumbromide (MTT)-viability assay. Corneas in the AMT mice had less inflammation, fewer PMN-like cells and fewer CD11b+, and Gr-1+ cells (P < 0.01), but a higher ratio of apoptotic to viable PMN-resembling cells (P < 0.01) than the T mice. Phagocytic removal of apoptotic PMN-like cells by macrophages was evident in the AMT group. After Cl 2 MDP-LIP treatment, the corneas had more cell debris and apoptotic cells with PMN-like morphology. The concentrations of IL-1 α, IL-2, CXCL1, and TNF-α were reduced in corneas of the AMT group as compared to that of the T group, while the concentration of CXCL2 was increased. Apoptosis of PMN-resembling cells was detected following cocultivation with AM, even when proinflammatory cytokines were present. Resolution of corneal inflammation in mice with necrotizing HSK after AMT is associated with increased apoptosis of PMN-like cells, reduction of pro-inflammatory cytokines, an increase of CXCL2, and increased removal of apoptotic PMN-like cells by macrophages.

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“…Ex vivo expanded cells must be transferred to the eye on a scaffold (Pellegrini et al, 1997), of which human amniotic membrane is the most commonly used (Tseng et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

Irani

Tian

Wang

et al. 2015

Experimental Eye Research

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Abbreviations: BSS ophthalmic balanced salt solution; DMEM Dulbecco's Modified Eagle's Medium; EGF epidermal growth factor; FBS fetal bovine serum; FDA fluorescein diacetate; PBS phosphate buffered saline; PCL polycaprolactone; pSi nanostructured porous silicon; SEM scanning electron microscopy; TEM transmission electron microscopy. To investigate loading of large-molecule biologics, murine BALB/c 3T3 cells, responsive to epidermal growth factor, insulin and transferrin, were seeded on composite materials. The cells showed significantly more proliferation at 48 h when seeded on composites loaded with these biologics, than on unloaded composites. No cell proliferation was observed on PCL alone, indicating the biologics had loaded into the pSi microparticles. Drug release, measured by ELISA for insulin, indicated a burst followed by a slower, continuous release over six days. When implanted under the rat conjunctiva, the most promising composite material did not cause significant neovascularization but did elicit a macrophage and mild foreign body

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Amniotic Membrane Transplantation With or Without Limbal Allografts for Corneal Surface Reconstruction in Patients With Limbal Stem Cell Deficiency

Cited by 633 publications

References 20 publications

Amniotic membrane transplantation in acute chemical burns

Amniotic membrane transplantation in acute chemical burns

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

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