Amoeba and Other Protozoa

Yudin, A. L.

doi:10.1007/978-1-4613-0503-3_1

Cited by 8 publications

(13 citation statements)

References 21 publications

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“…; http://accic.incras.ru/amoebae_en.htm). Following the standard procedure, the amoebae were cultivated at room temperature (RT) in the mineral Prescott medium (Prescott and Carrier ) and fed on the ciliate Tetrahymena pyriformis GL every 48 h (Yudin ).…”

Section: Methodsmentioning

confidence: 99%

“…We used cultures of four A. proteus strains of different geographical provenance (strains B, Cont, Neapol, CCAP 1503/8) and A. borokensis (strain Bor) from the Amoebae Culture Collection of the Institute of Cytology of the Russian Academy of Sciences (ACCIC; Goodkov et al 2015; http://accic.incras.ru/amoebae_en.htm). Following the standard procedure, the amoebae were cultivated at room temperature (RT) in the mineral Prescott medium (Prescott and Carrier 1964) and fed on the ciliate Tetrahymena pyriformis GL every 48 h (Yudin 1990).…”

Section: Amoebae Culturesmentioning

confidence: 99%

“…AMOEBA proteus , a paragon of the amoeba, has been a model object of cell biology for more than two centuries (reviewed by Hirshfield ; Jeon , ; Yudin and others). Yet, surprising as that may be, several quintessential issues of its biology, including some aspects of its cell cycle, remain unsolved.…”

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confidence: 99%

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The Chromatin Extrusion Phenomenon in Amoeba proteus Cell Cycle

Goodkov

Berdieva

Podlipaeva

et al. 2019

J Eukaryotic Microbiology

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Amoeba proteus is possibly the best known of all unicellular eukaryotes. At the same time, several quintessential issues of its biology, including some aspects of the cell cycle, remain unsolved. Here, we show that this obligate agamic amoebae and related species have a special type of cyclic polyploidy. Their nucleus has an euploid status only for a small fraction of the cell cycle, during metaphase and telophase. The rest of the time it has an aneuploid status, which is a consequence of polyploidization. Extrusion of “excess” chromatin from the nucleus in late interphase and during prophase results in depolyploidization. Such a strategy of life cycle in unicellular eukaryotes is thought to be the main mechanism of “resetting” the Muller's ratchet and is a satisfactory alternative to the meiotic recombination for agamic protists.

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Section: Methodsmentioning

confidence: 99%

Section: Amoebae Culturesmentioning

confidence: 99%

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The Chromatin Extrusion Phenomenon in Amoeba proteus Cell Cycle

Goodkov

Berdieva

Podlipaeva

et al. 2019

J Eukaryotic Microbiology

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“…The majority of laymen and all biologists are familiar with itas a curious (or not) theme in biology class, or a protozoological object and, moreover, a model organism for cell biology. A plethora of fundamental and applied studies carried out on this organism has been summarized in a number of fundamental reviews (Hirshfield, 1959;Jeon, 1973Jeon, , 1995Yudin, 1990;Anderson, 2017;etc. ).…”

Section: Amoeba Proteus and Cyclic Polyploidymentioning

confidence: 99%

“…Amoeba proteus (Amoebozoa, Tubulinea, Euamoebida, Amoebidae) ) is a free-living lobose amoeba that is traditionally regarded as an obligate agamic protist (Raikov, 1982;Yudin, 1990;Anderson, 2017). Nevertheless, the Amoeba species have a very special cell (=life) cycle (Fig.…”

Section: Amoeba Proteus and Cyclic Polyploidymentioning

confidence: 99%

Amoeba proteus and ploidy cycles: from simple model to complex issues

Berdieva¹,

Demin²,

Goodkov³

2019

Protistology

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This short topical review/opinion paper is inspired by the recent study of Amoeba proteus cell cycle. These obligate agamic amoebae have a special type of cyclic polyploidy-an alternation of unproportional polyploidization and depolyploidization, with the latter provided by chromatin extrusion from the nucleus into the cytoplasm. Here we discuss possible significance and mechanisms of this phenomenon, reconsider similar strategies of life cycles in other unicellular eukaryotes, and debate provocatively the fundamental issues, which could be brought up during its study-from functions of meiotic genes to evolution of sexual process to survival strategy of cancer cells.

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Unicellular associative conditioning: an interspecies analysis

Carrasco-Pujante

Bringas

Malaina

et al. 2020

Preprint

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The capacity to learn new systemic behaviour is a fundamental issue to understand the adaptive mechanisms involved in cellular evolution. We have recently observed, in a preliminary analysis, the emergence of conditioned behaviour in individual amoebae cells. In these experiments, cells were able to acquire new migratory conduct and remember it for long periods of their cellular cycle, forgetting it later on. Here, following a similar conceptual framework to that used by Pavlov in his experiments, we have exhaustively studied the migration trajectories of more than 2000 individual cells belonging to three different species: Amoeba proteus, Metamoeba leningradensis, and Amoeba borokensis. Fundamentally, we have analysed several properties of conditioned cells, such as the intensity of the responses, the directionality persistence, the total distance traveled, the directionality ratio, the average speed, and the persistence times. We have observed that these three species can modify the systemic response to a specific stimulus by associative conditioning. Our main analysis shows that such new behaviour is very robust and presents a similar structure of migration patterns in the three species, which was characterized by the presence of conditioning for long periods, remarkable straightness in their trajectories and strong directional persistence. Our quantitative results, compared with other studies on complex cellular responses in bacteria, protozoa, fungus-like organisms and metazoans, allow us to conclude that cellular associative conditioning might be a widespread characteristic of unicellular organisms. This finding could be essential to understand some key evolutionary principles involved in increasing the cellular adaptive fitness to microenvironments.

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Amoeba and Other Protozoa

Cited by 8 publications

References 21 publications

The Chromatin Extrusion Phenomenon in Amoeba proteus Cell Cycle

The Chromatin Extrusion Phenomenon in Amoeba proteus Cell Cycle

Amoeba proteus and ploidy cycles: from simple model to complex issues

Unicellular associative conditioning: an interspecies analysis

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