Amoebicidal Activities of Indonesian Medicinal Plants in Balikpapan, East Kalimantan

Wardana, Fendi Yoga; Sari, Defi Kartika; Adianti, Myrna; Permanasari, Adita Ayu; Tumewu, Lidya; Nozaki, Tomoyoshi; Widyawaruyanti, Aty; Hafid, Achmad Fuad

doi:10.5220/0008357700770082

Cited by 6 publications

(3 citation statements)

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“…According to the anti-amebic activity assay, dichloromethane extract had a higher selectivity index (SI) value than hexane extract. 8 Therefore, the fractionation of dichloromethane extract of C. sumatranum stem bark was carried out using Column Chromatography (CC). Following fractionation, we obtained 12 fractions, each of which was tested for anti-amebic activity by cellbased and enzymatic assays.…”

Section: Discussionmentioning

confidence: 99%

“…has antiamebic activity against E. histolytica cells with an IC 50 value of 22.07 µg/mL, CC 50 of 29.69 µg/mL and a Selective Index (SI) of 1.34. 8 Phytochemical studies of C. sumatranum obtained xanthone and anthraquinino-benzophenone such as cratoxyarborenones A-F; cratoxyarborequinones A-B; vismione B; 2,4,6-trihydroxybenzophenone 4-O-geranyl ether; δ-tocotrienol; betulinic acid, 9 and sumatranaxanthone A. 10 Other compounds that have also been isolated are cratosumatranone A-B; pruniflorone N; neriifolone B; isocudraniaxanthone B;…”

Section: Introductionmentioning

confidence: 99%

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In vitro Anti-Amebic Activity of Cage Xanthones from Cratoxylum sumatranum Stem Bark Against Entamoeba histolytica

Wardana¹,

Sari²,

Adianti³

et al. 2020

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Background: Amoebiasis is caused by Entamoeba histolytica, which is a pathogenic species living on human colon tissues. The development of new drugs for anti-amebic are still very needed for clinical treatment. Objective: This aims to identify the compounds in Cratoxylum sumatranum for their anti-amoeba activity. Materials and Methods: In this study we used bioactivity-guided isolation and structural analysis to identified anti-amebic compounds from dichloromethane extract of Cratoxylum sumatranum stem bark. Their anti-amebic activity was determined by an in vitro cell-based assay against Entamoeba histolytica and an enzymatic assay on NAD kinase. Results: Two known compounds from the cage xanthone groups, namely cochinchinoxanthone (1) and cochinchinone D (2), were isolated. The structures of the cage xanthone compounds were established by extensive spectroscopic data analysis. Compound (1) showed the greatest level of anti-amebic activity both in cell-based and enzymatic assay, yielding IC 50 values of 4.57 and 12.17 µg/mL, respectively. In contrast, compound (2) yielded IC 50 values of 5.19 and 12.60 µg/mL, respectively. Conclusion: When considering the demonstrated anti-amebic activities, it becomes apparent that these compounds, isolated from Cratoxylum sumatranum stem bark, have the potential to be further developed into effective anti-amebic medicine against Entamoeba histolytica.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro Anti-Amebic Activity of Cage Xanthones from Cratoxylum sumatranum Stem Bark Against Entamoeba histolytica

Wardana¹,

Sari²,

Adianti³

et al. 2020

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“…A Literature search showed a limited study of P. galeata regarding its metabolites and bioactivities. The methanolic extract of these plant leaves showed potential antiamoebic activity against Entamoeba hystolitica (11). There is no report on the antioxidant and cholinesterase inhibitory activity of this plant.…”

Section: Introductionmentioning

confidence: 95%

Antioxidant and Acetylcholinesterase Inhibitor Potentials of the Stem Extract of Pternandra galeata

Suciati

Inayah

Widyawaruyanti

et al. 2022

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Background: Pternandra galeata belongs to the family Melastomataceae. It is a native flowering plant in Borneo Island that serve as food for monkey habitat. There has been limited study on the medicinal and chemical properties of this plant. Objectives: We investigated the acetylcholinesterase inhibitory activity and evaluated the antioxidant activity of the ethanolic extract of Pternandra galeata stem. The total phenolic content in the sample was also determined. Methods: The acetylcholinesterase inhibitory assays were performed using Ellman’s method. Two different methods were used to evaluate the antioxidant activity of 2,2-diphenyl-1-picryl hydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The total phenolic content was determined by the Folin-Ciocalteu method by employing gallic acid as a reference. Results: The ethanolic extract of the P. galeata stems inhibited the AChE enzyme with an IC50value of 74.62 ± 0.89 µg/mL. The sample exhibited antioxidant activity in the DPPH assay with an IC50 value of 20.21 ± 0.08 µg/mL and 7.68 ± 0.09µg/mL in the ABTS scavenging assay. The total phenolic content was 164.71 ± 3.33 mg GAE/g extract. Conclusion: The ethanolic extract of the P. galeata stem can be a promising cholinesterase inhibitor and antioxidant for treating Alzheimer’s disease.

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Antiprotozoal Activity of Secondary Metabolites

Gulsen,

Touray,

Cimen

et al. 2024

Reference Series in Phytochemistry

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Amoebicidal Activities of Indonesian Medicinal Plants in Balikpapan, East Kalimantan

Cited by 6 publications

References 0 publications

In vitro Anti-Amebic Activity of Cage Xanthones from Cratoxylum sumatranum Stem Bark Against Entamoeba histolytica

In vitro Anti-Amebic Activity of Cage Xanthones from Cratoxylum sumatranum Stem Bark Against Entamoeba histolytica

Antioxidant and Acetylcholinesterase Inhibitor Potentials of the Stem Extract of Pternandra galeata

Antiprotozoal Activity of Secondary Metabolites

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