Amorphous calcium phosphate NPs-mediated macrophage response and modulate BMSCs osteogenesis

Abstract

Objective:To evaluate macrophage responses to ACP nanoparticles and the effect of ACP nanoparticle/macrophage-modulated environments on the osteogenic ability of BMSCs.Materials and methods:Macrophage polarization and cytokine expression were determined by SEM, cytoskeletal staining, RT-PCR and ELISA. ROS and autophagy expression were evaluated. TMT™ quantitative protein analysis was used to evaluate protein expression. BMSC osteogenic differentiation was detected by ALP staining, Alizarin Red solution staining and RT-PCR.ResultsACP nanoparticles changed macrophage morphology and polarized into M1 type. ACP nanoparticles promoted autophagy and induced the production of ROS at high concentrations. ACP nanoparticle/macrophage-modulated environments weakened the osteogenic ability of BMSCs.ConclusionACP nanoparticles polarize macrophages into the M1 phenotype. ACP nanoparticle/macrophage-modulated environments weaken the osteogenic ability of BMSCs.