Amorphous carbon nitride microband integrated in a microfluidic device for DNA biosensors applications

Abstract

This study presents the use of new kind of carbon electrode materials as ultramicroelectrodes (UMEs) in the field of electrochemical DNA biosensors which has already been proven to be effective in protocols to DNA sequences hybridization. In contrast to other carbon materials such as diamond like carbon, that are difficult to integrate in microfluidic devices due to their high temperature deposition, amorphous carbon nitride (a-CNx) is easily synthesized at room temperature on various materials using sputtering techniques. Here, we report a-CNx use as microband electrodes in Glass/PDMS microfluidic devices. a-CNx electrodes were activated and then biofuntionalized by covalent grafting of a DNA probe as self-assembled monolayer (SAM) with a view to future development of a detection platform targeting circulating DNA or RNA sequences in microfluidic channels.

An Integrated Multiple Electrochemical miRNA Sensing System Embedded into a Microfluidic Chip

“…In particular, it uses a microelectrode of 30 µm by 300 µm that acts as a working electrode (WE) and a millimetric electrode of 2 mm by 300 µm for the counter electrode (CE). This second long electrode has also the purpose of maintaining a resting potential stability between the CE and the electrolyte, thus operating also as a pseudo-reference electrode (RE) [23][24][25][26].…”