Amorphous silica coatings on magnetic nanoparticles enhance stability and reduce toxicity to<i>in vitro</i>BEAS-2B cells

Baber, Ori; Jang, Myoseon; Barber, David S.; Powers, Kevin

doi:10.3109/08958378.2011.592869

Cited by 38 publications

(24 citation statements)

References 49 publications

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“…In accordance with data reported in the literature (Baber et al, 2011), we confirmed that silica coating prevents the cytotoxicity of SPIONs as measured by the MTT assay. In our study, exposure to uncoated-SPIONs did not increase the quantity of acid organelles, while their cytotoxic action was detected at concentrations above 20 lg/mL.…”

Section: Discussionsupporting

confidence: 80%

“…High intracellular iron ion concentrations potentially lead to oxidative stress generated cytotoxicity (Sarkar et al, 2014). It is suggested that silica coating serves as a protective barrier that prevents iron ion dissolution from the SPIONs and thus lowers the cytotoxicity of SiO 2 -SPIONs (Baber et al, 2011). One possible explanation for different cellular effects of uncoated-and SiO 2 -SPIONs could be the fact that uncoatedSPIONs are internalized and degraded in lysosomes, which releases the toxic amount of iron ions, while the internalization of persistent SiO 2 -SPIONs did not lead to the release of a toxic amount of ions, but instead resulted in the intracellular accumulation of SiO 2 -SPIONs in acid organelles, which is a known outcome when cells endocytose hardly degradable material (Pentchev et al, 1980).…”

Section: Discussionmentioning

confidence: 99%

“…In medical applications, superparamagnetic iron oxide nanoparticles (SPIONs) have been reported to be a good choice (Sadhukha et al, 2013). SPIONs are often coated with a thin layer of amorphous silica in order to reduce iron dissolution, increase surface porosity and offer a good platform for surface functionalization (Baber et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

et al. 2017

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The pulmonary delivery of nanoparticles (NPs) is a promising approach in nanomedicine. For the efficient and safe use of inhalable NPs, understanding of NP interference with lung surfactant metabolism is needed. Lung surfactant is predominantly a phospholipid substance, synthesized in alveolar type II cells (ATII), where it is packed in special organelles, lamellar bodies (LBs). In vitro and in vivo studies have reported NPs impact on surfactant homeostasis, but this phenomenon has not yet been sufficiently examined. We showed that in ATII-like A549 human lung cancer cells, silica-coated superparamagnetic iron oxide NPs (SiO 2 -SPIONs), which have a high potential in medicine, caused an increased cellular amount of acid organelles and phospholipids. In SiO 2 -SPION treated cells, we observed elevated cellular quantity of multivesicular bodies (MVBs), organelles involved in LB biogenesis. In spite of the results indicating increased surfactant production, the cellular quantity of LBs was surprisingly diminished and the majority of the remaining LBs were filled with SiO 2 -SPIONs. Additionally, LBs were detected inside abundant autophagic vacuoles (AVs) and obviously destined for degradation. We also observed time-and dose-dependent changes in mRNA expression for proteins involved in lipid metabolism. Our results demonstrate that non-cytotoxic concentrations of SiO 2 -SPIONs interfere with surfactant metabolism and LB biogenesis, leading to disturbed ability to reduce hypophase surface tension. To ensure the safe use of NPs for pulmonary delivery, we propose that potential NP interference with LB biogenesis is obligatorily taken into account. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

et al. 2017

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“…Magnetic NPs could be protected from acidic erosion when coated with amorphous silica, stabilizing the NPs and reducing toxicities in in vitro cellular toxicity assays [62]. According to the charges of side chains, the same NPs exerted different toxicities in various cell types [60].…”

Section: Nanotechnology and Nanotoxicology In Retinopathy: Friendsmentioning

confidence: 99%

Nanotechnology and Nanotoxicology in Retinopathy

Lee

Kim

2011

IJMS

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Nanoparticles are nanometer-scaled particles, and can be utilized in the form of nanocapsules, nanoconjugates, or nanoparticles themselves for the treatment of retinopathy, including angiogensis-related blindness, retinal degeneration, and uveitis. They are thought to improve the bioavailability in the retina and the permeability of therapeutic molecules across the barriers of the eye, such as the cornea, conjunctiva, and especially, blood-retinal barriers (BRBs). However, consisting of multiple neuronal cells, the retina can be the target of neuronal toxicity of nanoparticles, in common with the central and peripheral nervous system. Furthermore, the ability of nanoparticles to pass through the BRBs might increase the possibility of toxicity, simultaneously promoting distribution in the retinal layers. In this regard, we discussed nanotechnology and nanotoxicology in the treatment of retinopathy.

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“…An amorphous silica coating of magnetic iron oxide nanoparticles was found to reduce cytotoxicity to human airway epithelial cells [162]. Whether these coatings of carbon nanotubes and iron oxide nanoparticles reduce hazards to human health in practice has so far not been determined.…”

Section: Hazard and Risk Reductionmentioning

confidence: 99%

Human health hazards of persistent inorganic and carbon nanoparticles

Reijnders

2012

J Mater Sci

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Persistent inorganic and carbon nanoparticles are increasingly engineered for applications and may also be present in conventional materials such as carbon black. Furthermore, they may originate from conventional non particulate materials by processes such as wear and tear. Persistent inorganic and carbon nanoparticles can be hazardous to humans. Relatively much research regards the hazards of inhaled nanoparticles. These may give rise to respiratory disease and to negative effects on other organs, including the cardiovascular system. Determinants of risk of inhaled nanoparticles include: number, size, surface characteristics, shape, structure, and the formation of assemblages. These determinants should preferentially be considered in exposure metrics. A major molecular mechanism underlying the inhalation hazard of nanoparticles is the generation of reactive oxygen species, but other mechanisms such as interactions with proteins and DNA may also contribute. Health hazards may also be linked to ingestion of persistent inorganic and carbon nanoparticles, dermal exposure and exposure of the eye. Standards for workplace exposure to persistent inorganic and carbon are currently emerging and there are options for hazard reduction by elimination and substitution of hazardous nanoparticles and by engineering controls.

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Amorphous silica coatings on magnetic nanoparticles enhance stability and reduce toxicity toin vitroBEAS-2B cells

Abstract: MNPs coated with amorphous silica are protected from acidic erosion. Correspondingly, the particle stability translates into reduced cytotoxicity and cellular influence on human airway epithelial cells.

Cited by 38 publications

References 49 publications

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Nanotechnology and Nanotoxicology in Retinopathy

Human health hazards of persistent inorganic and carbon nanoparticles

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Amorphous silica coatings on magnetic nanoparticles enhance stability and reduce toxicity toin vitroBEAS-2B cells

Abstract: MNPs coated with amorphous silica are protected from acidic erosion. Correspondingly, the particle stability translates into reduced cytotoxicity and cellular influence on human airway epithelial cells.

Cited by 38 publications

References 49 publications

Harmful at non-cytotoxic concentrations: SiO2-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Harmful at non-cytotoxic concentrations: SiO2-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Nanotechnology and Nanotoxicology in Retinopathy

Human health hazards of persistent inorganic and carbon nanoparticles

Contact Info

Product

Resources

About

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells

Harmful at non-cytotoxic concentrations: SiO₂-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells