Amount of enterotoxigenic Clostridium perfringens in meat detected by nested PCR

Miwa, Norinaga; Nishina, Takuya; Kubo, Shûichirô; Atsumi, Mikio; Honda, Hiroyasu

doi:10.1016/s0168-1605(98)00082-8

Cited by 43 publications

(33 citation statements)

References 16 publications

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“…Also meat, poultry and fish were detected as the common food. In another study, 37% of the meat and poultry samples were detected as positive for C. perfringens but different from our results 17% of the isolates were carrying cpe gene (27). Similarly Wen and McClane (2004) reported that 56 of 147 (38%) chicken meat were contaminated with C. perfringens and only one isolate was positive for cpe.…”

Section: Discussioncontrasting

confidence: 98%

“…On the other hand, similar to our results, 5% of the isolates were cpb2 positive in that study. Miwa et al (1998) detected C. perfringens in 42 of 50 (84%) of chicken samples in Japan. Likewise, in another study conducted in Japan, 70% of the Japanese retail raw meat samples were found to be contaminated with different numbers (MPN/g) of C. perfringens and 4% of the isolates were carrying cpe.…”

Section: Discussionmentioning

confidence: 97%

“…Epidemiological studies showed that more than 90% of C. perfringens outbreaks were associated with only meat and poultry products. (14,25,27,32). In various studies type A was reported to be the dominant type of C. perfringens isolated in poultry diseases (especially in necrotic enteritis) and from industry, processing line, products and environment worldwide (6,17,21,32).…”

Section: Discussionmentioning

confidence: 99%

“…As C. perfringens is an ubiquitous pathogen and common intestinal inhabitant of poultry, different stages of poultry production line can be evaluated as a contamination source even starting from the hatchery. Chicken carcass and meat parts may also be contaminated with C. perfringens by intestinal contents during slaughterhouse process especially on evisceration (6,7,17,27,31).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

GÖNCÜOĞLU¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of the study was to determine the prevalance and molecular characterization of Clostridium perfringens in edible offal of broiler. For this purpose a total of 90 samples, including 45 heart-livers and 45 gizzards were obtained from different supermarkets and transported to the laboratory in an ice box and tested at the same day. Culture technique was used for the isolation and selected biochemical tests, including acid phosphatase and reverse-CAMP tests was used for the identification of C. perfringens from the samples. Twenty one (46.7%) of 45 heart-liver and eight (17.7%) of 45 gizzards were found to be contaminated with C. perfringens. Isolates were molecular characterized by using multiplex PCR with alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) genes. Results showed that all isolates were carrying cpa gene and named as type A but none of them were positive for cpe gene. Only two gizzard isolates were positive for cpb2 gene. Our results indicate that edible offal of broiler are highly contaminated with C. perfringens type A which is mostly responsible for the foodborne poisoning worldwide. According to the high contamination rate edible offal should be considered as one of the important causes of C. perfringens type A foodborne disease.Key words: Broiler edible offal, Clostridium perfringens, molecular typing, multiplex PCR. Yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve moleküler tiplendirilmesiÖzet: Bu çalışmada yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve elde edilen izolatların moleküler karakterizasyonlarının belirlenmesi amaçlanmıştır. Çalışmada farklı süpermarketlerde satışa sunulan 45 kalp-karaciğer ve 45 taşlık olmak üzere toplam 90 yenilebilir iç organ soğuk zincir altında laboratuvara getirilerek aynı gün içerisinde analize alınmıştır. Örneklerden C. perfringens izolasyon ve identifikasyonunda klasik kültür tekniği ile asit fosfataz ve reverse-CAMP gibi biyokimyasal testler kullanılmıştır. Kırk beş kalp-karaciğer örneğinin 21'inin (% 46.7) ve 45 taşlık örneğinin sekizinin (% 17.7) C. perfringens ile kontamine olduğu saptanmıştır. Elde edilen izolatların alfa (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) ve enterotoksin (cpe) genlerine ait primer dizilimleri kullanılarak multipleks PCR ile moleküler tiplendirilmeleri yapılmıştır. Sonuçlar incelendiğinde bütün izolatların cpa geni taşıdığı belirlenmiş ve tip A olarak sınıflandırılmıştır, bununla beraber hiçbir izolatın cpe genine sahip olmadığı saptanmıştır. İki farklı taşlık örneğinden elde edilen izolatların cpa yanında cpb2 genini de taşıdıkları belirlenmiştir. Sonuç olarak yenilebilir piliç iç organlarının dünya genelinde gıda zehirlenmelerinde en sıklıkla rastlanan C. perfringens tip A ile yüksek oranda kontamine olduğu belirlenmiştir. Yüksek kontaminasyona bağlı olarak yenilebilir piliç iç organlarının C. perfringens tip A zehirlenmeleri açısından önemli birer kaynak oldukları saptanmıştır. Anahtar sözcükler: Clostridium per...

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Section: Discussioncontrasting

confidence: 98%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

GÖNCÜOĞLU¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…When the intestinal contents of broiler chickens are analyzed for the presence of C. perfringens, approximately 75% to 95% of the animals are found positive (Shane et al, 1984;Miwa et al, 1997;Craven et al, 2001a,b). When poultry meat is analyzed for C. perfringens, high percentages of positive meat samples are reported, in some cases up to 84% (Bean et al, 1996;Miwa et al, 1998;Craven et al, 2001a). It is suggested that colonization of poultry by C. perfringens is a very early event in the life of the animals, and can be transmitted within the integrated broiler chicken operation, starting from the hatchery (Craven et al, 2001a(Craven et al, ,b, 2003.…”

Section: Epidemiology Of C Perfringens In Poultrymentioning

confidence: 99%

Clostridium perfringensin poultry: an emerging threat for animal and public health

et al. 2004

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The incidence of Clostridium perfringens-associated necrotic enteritis in poultry has increased in countries that stopped using antibiotic growth promoters. Necrotic enteritis and the subclinical form of C. perfringens infection in poultry are caused by C. perfringens type A, producing the alpha toxin, and to a lesser extent type C, producing both alpha toxin and beta toxin. Some strains of C. perfringens type A produce an enterotoxin at the moment of sporulation and are responsible for foodborne disease in humans. The mechanisms of colonization of the avian small intestinal tract and the factors involved in toxin production are largely unknown. It is generally accepted, however, that predisposing factors are required for these bacteria to colonize and cause disease in poultry. The best known predisposing factor is mucosal damage, caused by coccidiosis. Diets with high levels of indigestible, water-soluble non-starch polysaccharides, known to increase the viscosity of the intestinal contents, also predispose to necrotic enteritis. Standardized models are being developed for the reproduction of colonization of poultry by C. perfringens and the C. perfringens-associated necrotic enteritis. One such model is a combined infection with Eimeria species and C. perfringens. Few tools and strategies are available for prevention and control of C. perfringens in poultry. Vaccination against the pathogen and the use of probiotic and prebiotic products has been suggested, but are not available for practical use in the field at the present time. The most cost-effective control will probably be achieved by balancing the composition of the feed.

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Clostridial Diseases

Boulianne¹,

Uzal²,

Opengart³

2019

Diseases of Poultry

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Amount of enterotoxigenic Clostridium perfringens in meat detected by nested PCR

Cited by 43 publications

References 16 publications

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

Clostridium perfringensin poultry: an emerging threat for animal and public health

Clostridial Diseases

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