Amount of maternal body fat significantly affected the quality of isolated mouse preimplantation embryos and slowed down their development

Kubandová, Janka; Číkoš, Štefan; Burkuš, Ján; Czikková, S.; Koppel, Juraj; Fabian, Denis

doi:10.1016/j.theriogenology.2013.10.014

Cited by 17 publications

(40 citation statements)

References 52 publications

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“…In addition to the phenotype differences, in EXF group the tendencies to increase in leptin and adiponectin blood concentrations were recorded, and in CL group a tendency to decrease in adiponectin blood concentration was observed. As shown in our previous study, fat dams showed significantly higher blood glucose (both EXN and EXF groups) and insulin (EXF group) concentrations than did controls (CN) [10]. The females with these four types of conditions were used as donor dams for the in vivo production of oocytes, zygotes (present study), preimplantation embryos (previous study [10]), and for the natural delivery of offspring (present study).…”

Section: Discussionmentioning

confidence: 56%

“…A two-generation dietary model based on overfeeding of experimental mice during prenatal and early postnatal development was used to produce females with various body conditions ( Fig.1) [10]. Adult female mice (30-35 day's old) of the parental generation (P) underwent hormonal synchronization (pregnant female's serum gonadotropin [eCG 5 IU intraperitoneally, Folligon; Intervet International, Boxmeer, Holland], followed 47 hours later by the administration of hCG [4 IU intraperitoneally, Pregnyl; Organon, Oss, Holland]).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…The body types include normal controls with physiological body weight and amount of body fat, mice with slightly increased body fat, mice with highly increased body fat and weight (obesity-like phenotype), and lean mice with decreased body fat and weight [9,10]. Using this model, we demonstrated the effect of altered maternal body condition on the development of in vivo-derived preimplantation embryos.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, our model simulates population heterogeneity in a manner analogous to naturally reproducing mammalian subjects (it uses outbred mice). Our model also allows for the study of maternal body condition and at the same time minimizing the impact of the composition of actual nutrition (during reproductive process, all animals are fed standard diet only), the impact of maternal aging (all females are at the early adulthood at the age standard used for reproductive studies), and the impact of hormonal treatment (only spontaneously ovulating donors of oocytes and pups are used) [10].…”

Section: Introductionmentioning

confidence: 99%

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The effect of maternal body condition on in vivo production of zygotes and behavior of delivered offspring in mice

et al. 2015

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Section: Discussionmentioning

confidence: 56%

Section: Animals and Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of maternal body condition on in vivo production of zygotes and behavior of delivered offspring in mice

et al. 2015

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“…Paternal obesity has previously been shown to reduce 1st 2nd and 3rd cleavage events, compaction, and fetal epiblast cell numbers in the developing embryo (6,7,54,59), while maternal obesity has been shown to reduce cleavage, compaction, blastocyst development, and blastocyst cell numbers (40,48,52,87,88). Therefore, it was perhaps not surprising that both paternal and maternal obesity alone or in combination also negatively impacted these measures.…”

Section: Alterations To Fetal Health From Combined Paternal and Matermentioning

confidence: 99%

When two obese parents are worse than one! Impacts on embryo and fetal development

McPherson

Bell

Zander-Fox

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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McPherson NO, Bell VG, Zander-Fox DL, Fullston T, Wu LL, Robker RL, Lane M. When two obese parents are worse than one! Impacts on embryo and fetal development. Am J Physiol Endocrinol Metab 309: E568 -E581, 2015. First published July 21, 2015; doi:10.1152/ajpendo.00230.2015.-The prevalence of overweight and obesity in reproductive-age adults is increasing worldwide. While the effects of either paternal or maternal obesity on gamete health and subsequent fertility and pregnancy have been reported independently, the combination of having both parents overweight/ obese on fecundity and offspring health has received minimal attention. Using a 2 ϫ 2 study design in rodents we established the relative contributions of paternal and maternal obesity on fetal and embryo development and whether combined paternal and maternal obesity had an additive effect. Here, we show that parental obesity reduces fetal and placental weights without altering pregnancy establishment and is not dependent on an in utero exposure to a high-fat diet. Interestingly combined parental obesity seemed to accumulate both the negative influences of paternal and maternal obesity had alone on embryo and fetal health rather than an amplification, manifested as reduced embryo developmental competency, reduced blastocyst cell numbers, impaired mitochondrial function, and alterations to active and repressive embryonic chromatin marks, resulting in aberrant placental gene expression and reduced fetal liver mtDNA copy numbers. Further understanding both the maternal cytoplasmic and paternal genetic interactions during this early developmental time frame will be vital for understanding how developmental programming is regulated and for the proposition of interventions to mitigate their effects. blastocyst; oocyte; sperm; embryo; fertility; infertility; obesity THE PREVALENCE OF OVERWEIGHT and obesity in reproductive-age adults is increasing worldwide (62). For example, in America it is estimated that 70.9% of males and 61.9% of women are classified as overweight/obese (62). The comorbidities associated with obesity have been well defined; however, until recently the impact on pregnancy and fetal development has been less recognized. Although the effects of either paternal or maternal obesity on gamete health and subsequent fertility and pregnancy have been reported independently, the combination of having both parents overweight/obese on fecundity and offspring health has received minimal attention. This information is essential, as the percentage of couples of reproductive age with both partners overweight/ obese is increasing and is the predominant situation in many countries (62).To date, there have only been three studies that have assessed the combined effects of maternal and paternal obesity on pregnancy and fetal health, two in human-assisted reproductive technology (ART) cohorts and one using a rodent high-fat diet model (41,67,76). These studies found no effect of maternal and paternal obesity on pregnancy establishment (41, 67, 76); however, when ...

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Maternal Obesity and Programming of the Early Embryo

Eckert

Velazquez

Fleming

2016

Parental Obesity: Intergenerational Programming and Consequences

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Amount of maternal body fat significantly affected the quality of isolated mouse preimplantation embryos and slowed down their development

Cited by 17 publications

References 52 publications

The effect of maternal body condition on in vivo production of zygotes and behavior of delivered offspring in mice

The effect of maternal body condition on in vivo production of zygotes and behavior of delivered offspring in mice

When two obese parents are worse than one! Impacts on embryo and fetal development

Maternal Obesity and Programming of the Early Embryo

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