<em>LINP1</em> lncRNA expression profile is modulated in response to DNA damage

Abstract

A: 2.4x10 5 cells were transfected with control or LINP1_1 oligos and processed after 48 h. Data are the mean of 3 independent experiments. SD, standard deviation. B: Representative phase contrast images of 46BR.1G1 cells 48 hours (t1) after transfection with CTRL and LINP1_1 LNA LINP1 lncRNA expression profile is modulated in response to DNA damage A low level of endogenous replicative DNA damage may impact gene expression programs and cell biology features relevant to cancer progression. This can be visualized by the comparison of DNA ligase I (LigI) defective 46BR.1G1 fibroblasts, deriving from a patient who died at 19 for lymphoma, and 7A3 cells, a 46BR.1G1 clone that stably expresses the ectopic wild-type LigI cDNA. LigI deficiency impairs maturation of newly synthesized DNA and increases the number of DSBs and gH2AX foci, two features associated with genome instability commonly found also in pre-neoplastic lesions.