&amp;lt;i&amp;gt;Bordetella holmesii&amp;lt;/i&amp;gt;: Comparison of Two Isolates from Blood and a Respiratory Sample

Bouchez, Valérie; Guiso, Nicole

doi:10.4236/aid.2013.32020

Cited by 8 publications

(10 citation statements)

References 42 publications

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“…Moreover, no traits specific to B . holmesii respiratory or blood isolates were identified previously [ 38 ]. Notably, while each of the respiratory isolates tested in the present study produced BipA, 3 of the 5 invasive isolates tested, including 2 ATCC strains, did not.…”

Section: Discussionmentioning

confidence: 99%

BipA Is Associated with Preventing Autoagglutination and Promoting Biofilm Formation in Bordetella holmesii

et al. 2016

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Bordetella holmesii causes both invasive and respiratory diseases in humans. Although the number of cases of pertussis-like respiratory illnesses due to B. holmesii infection has increased in the last decade worldwide, little is known about the virulence factors of the organism. Here, we analyzed a B. holmesii isolate that forms large aggregates and precipitates in suspension, and subsequently demonstrated that the autoagglutinating isolate is deficient in Bordetella intermediate protein A (BipA) and that this deletion is caused by a frame-shift mutation in the bipA gene. A BipA-deficient mutant generated by homologous recombination also exhibited the autoagglutination phenotype. Moreover, the BipA mutant adhered poorly to an abiotic surface and failed to form biofilms, as did two other B. holmesii autoagglutinating strains, ATCC 51541 and ATCC 700053, which exhibit transcriptional down-regulation of bipA gene expression, indicating that autoagglutination indirectly inhibits biofilm formation. In a mouse intranasal infection model, the BipA mutant showed significantly lower levels of initial lung colonization than did the parental strain (P < 0.01), suggesting that BipA might be a critical virulence factor in B. holmesii respiratory infection. Together, our findings suggest that BipA production plays an essential role in preventing autoagglutination and indirectly promoting biofilm formation by B. holmesii.

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Section: Discussionmentioning

confidence: 99%

BipA Is Associated with Preventing Autoagglutination and Promoting Biofilm Formation in Bordetella holmesii

et al. 2016

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“…The three human isolates were selected from the French National Reference Center for Whooping Cough and other Bordetelloses [19]. Bho1 was isolated in 1996 from the blood of a 20 years old man with sickle-cell anemia [48].…”

Section: Methodsmentioning

confidence: 99%

“…Partial genome sequences of Bho1, FR4020 and FR4101 isolates were available from Roche 454 sequencing [19]. Sequences of genes involved in lipid A biosynthesis were identified using the NCBI nucleotide and protein blast programs (blastn and blastp) ().…”

Section: Methodsmentioning

confidence: 99%

“…For the moment, it is not possible to differentiate B. holmesii isolates recovered from blood from isolates recovered from respiratory samples [ 17 , 18 , 19 ]. About 21 genomes of B. holmesii are available on The National Center for Biotechnology Information (NCBI) [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…First considered as close to B. pertussis on the basis of 16S DNA analysis, B. holmesii is now described in the same clade as B. hinzii , B. avium and B. trematum on the basis of whole genome single nucleotide polymorphism (SNP)-based analysis [ 21 ]. Most virulence factors usually produced by the classical Bordetella seem to be missing in B. holmesii except a Bordetella master virulence regulatory system ( bvg ), a filamentous hemagglutinin (FHA)-like protein, a bvg-intermediate phaseA protein ( bipA ) ortholog, and an alcaligin operon [ 19 , 22 , 23 , 24 , 25 ]. B. holmesii LPS have only been roughly studied by Van den Akker in 1998 who found them phenotypically and immunologically distinct from those of B. pertussis [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

Bouchez

Albitar-Nehme

Новиков

et al. 2017

IJMS

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Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography–mass spectrometry (GC–MS), and matrix-assisted laser desorption ionization–mass spectrometry (MALDI–MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2′ position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in B. pertussis and B. parapertussis strains, as well as in B. petrii isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data.

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Pertussis in Low and Medium Income Countries: A Pragmatic Approach

Guiso

Taieb

2019

Advances in Experimental Medicine and Biology

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<i>Bordetella holmesii</i>: Comparison of Two Isolates from Blood and a Respiratory Sample

Cited by 8 publications

References 42 publications

BipA Is Associated with Preventing Autoagglutination and Promoting Biofilm Formation in Bordetella holmesii

BipA Is Associated with Preventing Autoagglutination and Promoting Biofilm Formation in Bordetella holmesii

Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

Pertussis in Low and Medium Income Countries: A Pragmatic Approach

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