&amp;lt;i&amp;gt;Ex Vivo&amp;lt;/i&amp;gt; Characterization of the Action of Sideritis  Extract Using Electrical Activity in the Rat  Hippocampus Slice Preparation

Dimpfel, Wilfried; Schombert, Leonie; Feistel, B

doi:10.4236/pp.2016.710048

Cited by 7 publications

(6 citation statements)

References 15 publications

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“…The amplitude of the resulting population spike represents the number of recruited pyramidal cells. The response of the pyramidal cells to electric stimulation in the form of the amplitude of the population spike indicates activation as increase of the amplitude as reported earlier for Sideritis extract ( Dimpfel et al, 2016a ) or calming and sedating effects (attenuation of the amplitude). Of special interest is the response to theta burst stimulation (TBS) resulting in LTP, which relates to an increase of time- and space-dependent memory.…”

Section: Methodssupporting

confidence: 71%

Assessing the Quality and Potential Efficacy of Commercial Extracts of Rhodiola rosea L. by Analyzing the Salidroside and Rosavin Content and the Electrophysiological Activity in Hippocampal Long-Term Potentiation, a Synaptic Model of Memory

Dimpfel

Schombert²,

Panossian³

2018

Front. Pharmacol.

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Rhodiola rosea L. roots and rhizome extracts are active ingredients in adaptogenic herbal medicinal products (HMP) and dietary supplements for temporary relief of symptoms of stress, such as fatigue and weakness. R. rosea extract has a stimulating effect on the CNS, suggesting potential benefits on cognitive functions, memory, learning, and attention. The reproducible efficacy and quality of preparations of the underground parts of R. rosea depend on the highly variable content of the active markers, salidroside and rosavin, which affect the quality of HMP and dietary supplements. However, it is not clear which analytical markers are important for assessing the efficacy of R. rosea preparations intended for use in aging-induced mild cognitive disorders, such as attenuated memory, attention, and learning. Furthermore, the activity of various commercial R. rosea extracts has not been correlated with their content. Here, the biological activities of salidroside, rosavin, and seven commercial extracts of underground parts of R. rosea were assessed using a synaptic model of memory: long-term potentiation (LTP) of synaptic transmission in hippocampus slices. A high degree of variation in the content of all active markers was observed. One extract from China lacked rosavin, and there was even variation in the extracts from the Altai geographic region. In vitro, rosavin, salidroside and all tested R. rosea extracts potentiated electric stimulation of an intra-hippocampal electric circuit, which resulted in higher responses of the pyramidal cells in isolated hippocampus slices. Rosavin was more active at higher concentrations than salidroside; while, salidroside was more effective at lower concentrations. The highest content of both active markers was found in the extracts that were active at the lowest concentrations tested; while, some extracts contained some other compounds that presumably reduced the efficacy due to antagonistic interactions. Standardized content of active markers is necessary for the quality control of herbal preparations containing R. rosea extracts, but insufficient for assessment of their potential efficacy. Additional bioassays are needed to assure the reproducible pharmacological activity of R. rosea extracts; therefore, the LTP of synaptic transmission in hippocampus slices may serve as a validation tool for the quality control of R. rosea extracts.

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Section: Methodssupporting

confidence: 71%

Assessing the Quality and Potential Efficacy of Commercial Extracts of Rhodiola rosea L. by Analyzing the Salidroside and Rosavin Content and the Electrophysiological Activity in Hippocampal Long-Term Potentiation, a Synaptic Model of Memory

Dimpfel

Schombert²,

Panossian³

2018

Front. Pharmacol.

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“…Hydroalcoholic extracts were able to inhibit the reuptake of the monoamine neurotransmitters noradrenaline, dopamine and serotonin in vitro ( Feistel & Appel, 2013 ; Knörle, 2012 ). Furthermore, they showed antidepressant and psychostimulating effects, as well as a modulation of AMPA-dependent neurotransmission in rats ( Dimpfel, 2013 ; Dimpfel, Schombert & Feistel, 2016a ). In mice, cognitive enhancement and Aβ-counteracting effects were observed ( Hofrichter et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Sideritis scardicaextracts inhibit aggregation and toxicity of amyloid-βinCaenorhabditis elegansused as a model for Alzheimer’s disease

Heiner

Feistel

2018

PeerJ

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BackgroundBeyond its traditional uses in the Balkan area, Sideritis scardica (known as Greek mountain tea, Lamiaceae) is currently extensively investigated for its pharmacological activity in the central nervous system. Antidepressant, psychostimulating, cognition-enhancing and neuroprotective properties have been described. In this study, we tested hydroalcoholic extracts of S. scardica for their potential to counteract amyloid-β toxicity and aggregation, which plays a crucial role in the pathogenesis of Alzheimer’s disease.MethodsFor this purpose, we have chosen the nematode Caenorhabditis elegans, which is used as a model organism for neurodegenerative diseases. The concentration of different polyphenols in extracts prepared from water, 20, 40, 50, and 70% ethanol was analysed by HPLC. Additionally, polar and unpolar fractions were prepared from the 40% ethanolic extract and phytochemically analysed.ResultsEssentially, the contents of all measured constituents increased with the lipophilicity of the extraction solvents. Treatment of transgenic C. elegans strains expressing amyloid-β with the extracts resulted in a reduced number of peptide aggregates in the head region of the worms and alleviated toxicity of amyloid-β, observable through the degree of paralysed animals. The mid-polar extracts (40 and 50% ethanol) turned out be the most active, decreasing the plaque number by 21% and delaying the amyloid-β-induced paralysis by up to 3.5 h. The more lipophilic extract fractions exhibited higher activity than the hydrophilic ones.DiscussionSideritis scardica extracts demonstrated pharmacological activity against characteristics of Alzheimer’s disease also in C. elegans, supporting current efforts to assess its potential for the treatment of cognitive decline. The active principle as well as the mode of action needs to be investigated in more detail.

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“…By far Sideritis scardica is the most studied species in this respect. It was shown to have a positive effect on Attention-Deficit Hyperactivity Disorder [112], in the treatment of cognitive impairment and improving the cognition [116][117][118], as well as for improvement of stress tolerance [119]. Latte [155] reviewed the use of S. scardica for the treatment of neurological disorders and neurodegenerative diseases.…”

Section: Pharmacologic Activities On the Central Nervous System Diseasesmentioning

confidence: 99%

Genus Sideritis, section Empedoclia in southeastern Europe and Turkey – studies in ethnopharmacology and recent progress of biological activities

et al. 2019

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Background Over the last two decades there has been a substantial increase of the number of studies on the species of genus Sideritis. Species of section Empedoclia, occurring in the Eastern Mediterranean region and in part of Western Asia possess some remarkable characteristics and are known as valuable medicinal plants used by local people in the traditional medicine and for herbal tea. The objective of the review is to make a survey on the recent studies on the ethnopharmacology and biological activity of the species in Southeastern Europe and in Turkey, which is the center of distribution and their main occurrence. Main body The review focuses on the ethnopharmacology and biological activities of the species of interest. The survey revealed that a total of 47 species belonging to section Empedoclia have been studied either in ethnopharmacological aspect, or in relation to their biological activities, or both. Most species have been used traditionally by the local people as herbal tea or for treatment of various health problems, most frequently flu, cold and respiratory diseases. Sideritis species demonstrate numerous biological activities and are promising for use in the therapy of many diseases and health disorders. Antioxidant activity was found in 40 species, antimicrobial and antibacterial activityin 27 species, anti-inflammatoryin 14 species, antifungalin 8 species, cytotoxicin 7 species. There were also some other, more specific biological activities, found in a few species, but considered promising for further studies and application. Short conclusion The species of genus Sideritis, section Empedoclia have been used by local people as herbal tea and in traditional medicine since long time ago. People are taking advantage of the high species diversity and are aware of their useful properties. Much more information is available on the biological activities of the target species than on their traditional uses. Most species demonstrate various biological activities and are of substantial interest for further studies on their pharmacological properties and their potential for pharmacy and medicine.

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<i>Ex Vivo</i> Characterization of the Action of Sideritis Extract Using Electrical Activity in the Rat Hippocampus Slice Preparation

Cited by 7 publications

References 15 publications

Assessing the Quality and Potential Efficacy of Commercial Extracts of Rhodiola rosea L. by Analyzing the Salidroside and Rosavin Content and the Electrophysiological Activity in Hippocampal Long-Term Potentiation, a Synaptic Model of Memory

Assessing the Quality and Potential Efficacy of Commercial Extracts of Rhodiola rosea L. by Analyzing the Salidroside and Rosavin Content and the Electrophysiological Activity in Hippocampal Long-Term Potentiation, a Synaptic Model of Memory

Sideritis scardicaextracts inhibit aggregation and toxicity of amyloid-βinCaenorhabditis elegansused as a model for Alzheimer’s disease

Genus Sideritis, section Empedoclia in southeastern Europe and Turkey – studies in ethnopharmacology and recent progress of biological activities

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