<i>Flash microbiocide</i>: A Rapid and Economic Method for Determination of MBC and MFC

Abstract

Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that the establishment of innovative methodologies could be more practical and cheaper. The new methodology Flash microbiocide is an assay in which one aliquot from 96 well plate of Minimal Inhibitory Concentration test is transferred to another plate containing different culture medium. The correspondence with the reference methods described in the National Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was achieved, denoting the efficiency of this fast and simple method.

Antimicrobial Activities of Some Marine Macroalgae Species from Iskenderun Bay

“…The high activity of the MICs and MBCs of macroalgae extracts against bacteria and fungi which were identified in the present study, are consistent with the results of MICs and MBCs / MFCs that were previously reported in other studies (Lambert et al, 2001;Stirk et al, 2007;El-Baky et al, 2009;Ertürk and Taş, 2011;Salem et al, 2011;Hernandes et al, 2013;Venugopa et al, 2014;Al-Judaibi et al, 2014;Zahrani et al, 2014;Veeramohan et al, 2017;Srikong et al, 2017;Martins et al 2018;Erdogan Eliuz et al, 2019;El-Sheekh et al,2020). Pesando (1990) reported that all types of antimicrobial metabolites including (green, brown red algae) exist in temperate or tropical marine.…”

Effect of Gold Nanostars Plus Amikacin against Carbapenem-Resistant Klebsiella pneumoniae Biofilms

“…On completion of the incubation, columns with no color change were scored as above the MIC value and the fluorescence was measured using an excitation wavelength of 560 nm and emission wavelength of 590 nm. Minimum bactericidal concentrations (MBCs) were determined using the flash microbicide method, previously described [ 36 ]. The MBC is defined as the minimum concentration required to kill a certain bacterial concentration.…”

Sanguiin H-6 Fractionated from Cloudberry (Rubus chamaemorus) Seeds Can Prevent the Methicillin-Resistant Staphylococcus aureus Biofilm Development during Wound Infection

“…Minimum bactericidal concentration (MBC) was determined using previously described broth microdilution method with some modifications [ 44 ]. Briefly, 20 μL of each well after 24-h incubation were mixed with 180 μL of tryptic soy broth in a new 96-well plate and incubated statically at 37 °C and 5% CO 2 for 24 h. After incubation, 100 μL of each well were mixed with 100 μL of tryptic soy broth with 20% alamarBlue, added to a new 96-well flat-bottom plate and incubated at 37 °C and 90 rpm for 30 min.…”