<i>In Vitro</i> Evaluation of Bacterial Adhesion to Dental and Stainless-Steel Surfaces

Abstract

This study aimed to describe the factors associated with biofilms formation in dental pathology by comparison of bacterial growth on dental and stainlesssteel surfaces. We studied in vitro the behavior of Staphylococcus aureus Métis in order to observe the capacity of adhesion, to evaluate quantitatively the potential of proliferation and to compare the behavior of this germ in contact with the two surfaces. The biomaterials used were cylinders in Stainless steel (AISI 316L), dental fragments and stainless-steel fragments, all were disinfected for 15 minutes and then sterilized in a wet autoclave at 120˚C for 30 min. Macroscopic observation with a binocular magnifier of bacterial proliferation was carried out regularly after 6 h and 24 h of incubation. Observation by optical microscope based on GRAM staining made it possible to visualize the presence or absence of bacteria and to differentiate them. The adhesion of Staphylococcus aureus Méti S on dental fragments was compared to the one obtained on stainless steel fragments. We also carried a Bacterial count by optical dosing. The results show that the ability of this germ to colonize and develop biofilms on surfaces depends mainly on the characteristics of the surface. Rough surfaces as dental surface are more likely to developing biofilms than smooth surfaces like stainless-steel surface.

Plasma treatment effects on destruction and recovery of Porphyromonas gingivalis biofilms

“…Stainless steel was also utilized for studying adhesion of S . aureus to its surface for dental application [ 39 ]. Additional biomaterials used for making dental implants are titanium (Ti) and its alloy, Ti6Al4V.…”

Manipulating Bacterial Biofilms Using Materiobiology and Synthetic Biology Approaches

“…However, in 2021, Aouame et al reported that the bacteria adhesion and biofilm formation mainly depend on surface characteristics. Bacteria are more likely to adhere to and develop biofilm on rough dental surfaces than on smooth stainless-steel surfaces ( Aouame et al, 2021 ). Different materials, strains, and test methods were applied in these studies, making it difficult to compare them.…”

“…It is worth noticing that, when choosing their behaviors, bacteria would seem to comprehensively consider all surface properties. These surface properties might only have one or two factors predominant bacterial behaviors or, sometimes, may all have a contribution ( Spriano et al, 2017 ; Wassmann et al, 2017 ; Aouame et al, 2021 ). Besides, the testing conditions, such as culture media used, either flow system or static system, will all affect the bacterial attachment ( Senevirathne et al, 2021 ).…”