<i>In Vitro</i> Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

Abstract

of indole-3-butyric acid (IBA). The highest shoot initiation rates were achieved with apple cultivar "Principe Grande" (55%) and "Barburiña" pear cultivar (50%); the lowest rate was obtained with apple cultivar "Camoesa" (11%). Initiation rates for shoots culture varied between 22% and 37% for the other cultivars. The cytokinins BA, zeatin, 2-isopentyladenine (2iP) and thidiazuron (

An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants

“…TDZ induced abnormalities such as hyperhydric shoots, dwarfing, and shoot fasciation [23,24]. However, the level of abnormality was dependent on the TDZ concentration as well as the apple genotype [25]. There is no direct relationship between shoot regeneration and callus size, there is a direct relationship between shoot regeneration and but callus features.…”

In vitro tissue culture of apple and other Malus species: recent advances and applications

“…Chitosan has been widely used in orchid tissue cultures and has a wide range of biological properties, including antimicrobial and promotion of plant growth (Uthairatanakij et al 2007). TDZ multiplied shoots more effectively than BA in eight Galician traditional apple cultivars, but its optimal concentration depended on the cultivar (Table 2; Lizárraga et al 2017). However, the percentage of hyperhydric shoots was highest in five out of eight cultivars while in two cultivars ('Ollo Mouro', 'José Antonio'), it exceeded 50% when TDZ was added to the shoot multiplication medium (Lizárraga et al 2017).…”

“…Method 2: shoot tips (2-3 cm) → soapy water → RW → 0.1% HgCl 2 5, 7 or 10 min → rinses NR Romadanova et al (2016a) Dormant cuttings → RTW 20 min → 8% (w/v) Capluq-50 fungicide solution time NR → drying and wrapping in plastic film and storage at 4 °C for 1 month → sprouting of cuttings in a phytotron (16-PP, 25/18 °C) → cutting new shoots → 10% NaOCl + 1 drop of Tween-20 15 min → SDW 3 × Lizárraga et al (2017) Axillary buds, terminal buds, shoot tips (spring and winter) → 1-2% NaOCl time NR → rinses NR Modgil and Thakur (2017) Cotyledons, stems, leaves → 2.6-5.3% NaOCl + Tween-20 5-10 min → DW 3 × (5 min each wash) In vitro shoots (size, age NR) ½ MS + 0.3 mg/l IBA or 0.1 mg/l NAA + 2 g/l AC (RIM). 2% sucrose.…”

“…TDZ multiplied shoots more effectively than BA in eight Galician traditional apple cultivars, but its optimal concentration depended on the cultivar (Table 2; Lizárraga et al 2017). However, the percentage of hyperhydric shoots was highest in five out of eight cultivars while in two cultivars ('Ollo Mouro', 'José Antonio'), it exceeded 50% when TDZ was added to the shoot multiplication medium (Lizárraga et al 2017). Geng et al (2015) noted the importance of GA 3 for shoot development in three rootstocks released from the Cornell-Geneva (Geneva ® series) breeding program, depending on the light source, while red light induced significantly more shoots, and longer shoots, than when white or blue light was used, but this depended on the cultivar.…”

Fast and Effective Thermotherapy Treatment for <i>In Vitro</i> Virus Eradication in Apple and Pear Trees

“…Then they were transferred to jars and they remained subcultured every four weeks until they multiplied. All cultures were transferred to a growth chamber with a 16 hours photoperiod under the following conditions: 24˚C ± 1˚C day, with a light irradiancy of 40 µmol•m for "José Antonio" and "Príncipe Grande", all of them apple cultivars, and mineral medium MS with BA 1 mg•L −1 for pears cultivars "Barburiña" and "Manteca Oscura" [20].…”